Laboratory Applications in Microbiology: A Case Study Approach / Edition 2

Other Format (Print)
Rent
Rent from BN.com
$17.60
(Save 86%)
Est. Return Date: 09/28/2014
Used and New from Other Sellers
Used and New from Other Sellers
from $24.30
Usually ships in 1-2 business days
(Save 81%)
Other sellers (Other Format)
  • All (14) from $24.30   
  • New (3) from $77.39   
  • Used (11) from $24.30   

More About This Textbook

Overview

Laboratory Applications in Microbiology: A Case Study Approach now includes a photo atlas with more than 250 full-color images! This lab uses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.

Read More Show Less

Product Details

  • ISBN-13: 9780073402376
  • Publisher: McGraw-Hill Higher Education
  • Publication date: 1/21/2011
  • Format: Spiral Bound
  • Edition description: Older Edition
  • Edition number: 2
  • Pages: 688
  • Sales rank: 738,104
  • Product dimensions: 8.70 (w) x 10.70 (h) x 1.00 (d)

Table of Contents

ESSENTIAL LABORATORY SKILLS

Exercise 1 Safety Considerations in the Microbiology Laboratory

CASE STUDIES

Laboratory-Acquired Infection with Escherichia coli O157:H7—New York, 2004

Laboratory-Acquired Brucellosis—New York, 2001Ocular Vaccinia Infection of a Laboratory Worker—Philadelphia, 2004

Exercise 2 Microscopy and Measurement of Microscopic Specimens

CASE STUDY

Excerpt of Letters from Anton van Leeuwenhoek to the Royal Society of London for the Improvement of Natural Knowledge
SURVEY OF MICROORGANISMS

Exercise 3 Identification and Classification of Algae

CASE STUDY

Oregon Harmful Bloom Monitoring Project

Exercise 4 Survey of Medically Important Protozoa

CASE STUDY

Acanthamoeba Keratitis—Multiple States

Exercise 5 Ubiquity of Bacteria

CASE STUDY

Engineering Infection Control through Facility Design

Exercise 6 Identification and Classification of Fungi

CASE STUDY

Outbreak of Acute Febrile Respiratory Illness among College Students—Acapulco, Mexico, March 2001
MANIPULATION, STAINING, AND OBSERVATION OF MICROORGANISMS

Exercise 7 Aseptic Techniques

CASE STUDY

Multiple Misdiagnoses of Tuberculosis Resulting from Laboratory Error—Wisconsin, 1996

Exercise 8 Pure Culture Techniques

CASE STUDY

Neonatal Tetanus—Montana, 1998

Exercise 9 Simple Staining, Negative Staining, and Gram Staining

CASE STUDY

Identification of Bacteria Responsible for the Outbreak of Gastrointestinal Disease

Exercise 10 Capsular Staining

CASE STUDY

Pneumococcal Sepsis after Autosplenectomy in a Girl with Systemic Lupus Erythematosus—2005

Exercise 11 Acid-Fast and Endospore Staining

CASE STUDIES

Mycobacterium tuberculosis Transmission in a Newborn Nursery and Maternity Ward—New York City, 2003Inhalation Anthrax Associated with Dried Animal Hides—Pennsylvania and New York City, 2006
ENVIRONMENTAL INFLUENCES ON THE GROWTH OF MICROORGANISMS

Exercise 12 Viable Plate Count

CASE STUDY

Multidrug-Resistant Salmonella Typhimurium Infection from Milk Contaminated after Pasteurization

Exercise 13 Cultivation of Anaerobes

CASE STUDY

Botulism from Home-Canned Bamboo Shoots—Nan Province, Thailand, March 2006

Exercise 14 Temperature Effects on Bacterial Growth and Survival

CASE STUDY

Outbreak of Gastroenteritis Associated with Consumption of Alaskan Oysters

Exercise 15 pH and Microbial Growth

CASE STUDY

Garlic-in-Oil-Associated Botulism: Episode Leads to Product Modification

Exercise 16 Effects of Osmotic Pressure on Bacterial Growth

CASE STUDY

Outbreak of Staphylococcal Food Poisoning Associated with Precooked Ham—Florida, 1997
CONTROL OF MICROBIAL GROWTH

Exercise 17 Lethal Effects of Ultraviolet Light

CASE STUDY

Gastrointestinal Outbreak Traced to Interactive Fountain—New York, March 2006

Exercise 18 Evaluation of Disinfectants: Use-Dilution Method

CASE STUDY

Nine Episodes of Anaphylaxis Following Cystoscopy Caused by a High-Level Disinfectant—2004

Exercise 19 Effectiveness of Hand Scrubbing

CASE STUDY

Puerperal Fever—Vienna, Austria, 1847

Exercise 20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and E-Test Methods

CASE STUDY

Methicillin-Resistant Staphylococcus aureus Infections among Tattoo Recipients—Ohio, Kentucky, and Vermont, 2004–2005
EPIDEMIOLOGY

Exercise 21 Phage Typing of Bacteria

CASE STUDY

Salmonella Serotype Enteritidis Infections among Workers Producing Poultry Vaccine—Maine, November–December, 2006

Exercise 22 Simulated Epidemic

CASE STUDY

Import-Associated Measles Outbreak—Indiana, May–June 2005

Exercise 23 Morbidity and Mortality Weekly Report

CASE STUDY

Multistate Outbreak of Mumps—United States, January 1–October 7, 2006
MICROBIAL GENETICS

Exercise 24 Bacterial Transformation

CASE STUDY

Staphylococcus aureus Resistant to Vancomycin—United States, 2002

Exercise 25 The Ames Test

CASE STUDY

West Nile Virus Update—United States, January 1–November 13, 2007
APPLIED MICROBIOLOGY

Exercise 26 DNA Extraction from Bacterial Cells

CASE STUDY

Multistate Outbreaks of Salmonella Infections Associated with Raw Tomatoes Eaten in Restaurants—United States, 2005–2006

Exercise 27 Measures of Water Quality: Most Probable Number Procedure

CASE STUDY

E. coli Contamination of Water Supply—Frazier Park, California, 2007

Exercise 28 Measures of Water Quality: Membrane Filtration Method

CASE STUDY

Fecal Contamination of Airline Drinking Water—2005

Exercise 29 Measures of Milk Quality: Methylene Blue Reductase Test

CASE STUDY

Salmonella Typhimurium Infection Associated with Raw Milk and Cheeses Consumption—Pensylvania, 2007

Exercise 30 Bacterial Counts of Food

CASE STUDY

Multiple Outbreaks of Food Poisoning—1989–2006
MEDICAL MICROBIOLOGY

Exercise 31 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae

CASE STUDY

Outbreaks of Escherichia coli O157:H7 Associated with Petting Zoos—North Carolina, Florida, and Arizona, 2004 and 2005

Exercise 32 Isolation and Identification of Staphylococci

CASE STUDY

Community-Associated Methicillin-Resistant Staphylococcus aureus Infection among Healthy Newborns—Chicago and Los Angeles County, 2004

Exercise 33 Isolation and Identification of Streptococci

CASE STUDY

Invasive Streptococcus pyogenes after Allograft Implantation—Colorado, 2003
IDENTIFICATION OF UNKNOWN BACTERIA

Exercise 34 Identification of Bacterial Unknowns

CASE STUDY

Respiratory Disease Strikes Legionnaires Convention—Philadelphia, 1976
LABORATORY TECHNIQUES, REAGENTS, AND ASSAYSAnalysis of Bacterial Cultures Based on Morphological Characteristics

Exercise 35 Colony Morphology

Exercise 36 Growth in Solid and Liquid Media
Staining Techniques Used for the Microscopic Examination of Bacteria

Exercise 37 Simple Stain

Exercise 38 Negative Stain

Exercise 39 Capsule Stain

Exercise 40 Gram Stain

Exercise 41 Endospore Stain

Exercise 42 Acid-Fast Stain

Exercise 43 Motility Methods: Wet Mount and Hanging Drop

Exercise 44 Flagella Stain
Techniques for Inoculation of Media

Exercise 45 Streak Plate Isolation

Exercise 46 Loop Dilution

Exercise 47 Spread Plate
Commonly Used Differential and Selective Media

Exercise 48 Fluid Thioglycollate Media

Exercise 49 Mannitol Salt Agar

Exercise 50 MacConkey Agar

Exercise 51 Desoxycholate Agar

Exercise 52 Endo Agar

Exercise 53 Eosin Methylene Blue Agar

Exercise 54 Hektoen Enteric Agar

Exercise 55 Xylose Lysine Desoxycholate Agar

Exercise 56 Blood Agar

Exercise 57 Motility Media

Exercise 58 SIM Medium

Exercise 59 Kligler’s Iron Agar

Exercise 60 Triple Sugar Iron Agar

Exercise 61 Lysine Iron Agar

Exercise 62 Litmus Milk
Commonly Used Biochemical Tests

Exercise 63 Oxidation-Fermentation Test

Exercise 64 Phenol Red Broth

Exercise 65 Purple Broth

Exercise 66 Methyl Red and Voges-Proskauer Tests

Exercise 67 Catalase Test

Exercise 68 Oxidase Test

Exercise 69 Nitrate Reduction Test

Exercise 70 Coagulase Test

Exercise 71 Citrate Test

Exercise 72 Malonate Test

Exercise 73 Decarboxylation Test

Exercise 74 Phenylalanine Deaminase Test

Exercise 75 Bile Esculin Test

Exercise 76 Starch Hydrolysis

Exercise 77 ONPG Test

Exercise 78 Urease Test

Exercise 79 Casease Test

Exercise 80 Gelatinase Test

Exercise 81 DNase Test

Exercise 82 Lipase Test

Exercise 83 CAMP Test

Exercise 84 PYR Test
Commercial Identification Systems

Exercise 85 API 20E System

Exercise 86 Enterotube II System
Antimicrobial Susceptibility Tests

Exercise 87 Antibiotic Disk Sensitivity Tests for Bacterial Identification

Exercise 88 β-Lactamase Test
Quantitative Techniques

Exercise 89 Viable Plate Count

Exercise 90 Direct Cell CountAppendix A Spectrophotometric Determination of Bacterial Growth: Use of the Spectrophotometer
Appendix B Use of Pipettes in the Laboratory
Appendix C Preparation of Culture Media
Appendix D Media, Reagents, and Stain Formulas
Appendix E Data Sheet for Unknown Identification
Glossary
Index
Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)