Laboratory Experiments in Microbiology / Edition 10

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Containing 57 thoroughly class-tested and easily customizable exercises,Laboratory Experiements in Microbiology: Tenth Edition provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The Tenth Edition features an updated art program and a full-color design, integrating valuable micrographs throughout each exercise. Additionally, many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style to better visually engage students. Laboratory Reports for each exercise have been enhanced with new Clinical Applications questions, as well as question relating to Hypotheses or Expected Results. Experiments have been refined throughout the manual and the Tenth Edition includes an extensively revised exercise on transformation in bacteria using pGLO to introduce students to this important technique.

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Editorial Reviews

A laboratory companion to , Sixth Edition by Tortora, Funke, and Case. The manual contains 56 class- tested exercises, including all those recommended by the Committee on Undergraduate and Graduate Education of the American Society for Microbiology. Sections cover microscopy, staining methods, cultivation of bacteria, microbial metabolism, microbial growth, control of microbial growth, microbial genetics, the microbial world, viruses, interaction of microbe and host, immunology, microorganisms and disease, and microbiology and the environment. A color photo quiz requires students to identify an unknown. Spiral wire binding. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780321794383
  • Publisher: Benjamin Cummings
  • Publication date: 1/14/2012
  • Format: Spiral Bound
  • Edition description: 10
  • Edition number: 10
  • Pages: 496
  • Sales rank: 113,270
  • Product dimensions: 8.20 (w) x 10.70 (h) x 0.80 (d)

Meet the Author

Ted R. Johnson is a professor of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he teaches courses in microbiology and immunology. He has taught at St. Olaf for 34 years and previously taught at Mankato State University. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several abroad semester and interim programs. His research focuses on the immune response to virally induced cancer in various animal models.

Christine L. Case is a registered microbiologist and a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 41 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.

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Table of Contents



1. Use and Care of the Microscope

2. Examination of Living Microorganisms

II. Handling Bacteria

3. Microbes in the Environment

4. Transfer of Bacteria: Aseptic Techniques


5. Preparation of Smears and Simple Staining

6. Negative Staining

7. Gram Staining

8. Acid-Fast Staining

9. Structural Stains (Endospore, Capsule, Flagella)

10. Morphologic Unknown


11. Isolation of Bacteria by Dilution Techniques

12. Special Media for Isolating Bacteria


13. Carbohydrate Catabolism

14. Fermentation

15. Protein Catabolism, Part 1

16. Protein Catabolism, Part 2

17. Respiration

18. Unknown Identification and Bergey’s Manual


19. Oxygen and the Growth of Bacteria

20. Determination of a Bacterial Growth Curve: The Role of Temperature

21. Biofilms


22. Physical Methods of Control: Heat

23. Physical Methods of Control: Ultraviolet Radiation

24. Chemical Methods of Control: Disinfectants and Antiseptics

25. Chemical Methods of Control: Antimicrobial Drugs

26. Effectiveness of Hand Scrubbing


27. Regulation of Gene Expression

28. Isolation of Bacterial Mutants

29. Transformation of Bacteria

30. DNA Fingerprinting

31. Genetic Engineering

32. Ames Test for Detecting Possible Chemical Carcinogens


33. Fungi: Yeasts and Molds

34. Phototrophs: Algae and Cyanobacteria

35. Protozoa

36. Parasitic Helminths


37. Isolation and Titration of Bacteriophages

38. Plant Viruses


39. Epidemiology

40. Koch’s Postulates


41. Innate Immunology

42. Agglutination Reactions: Slide Agglutination

43. Agglutination Reactions: Microtiter Agglutination

44. ELISA Technique


45. Bacteria of the Skin

46. Bacteria of the Respiratory Tract

47. Bacteria of the Mouth

48. Bacteria of the Gastrointestinal Tract

49. Bacteria of the Genitourinary Tract

50. Identification of an Unknown from a Clinical Sample

51. Rapid Identification Methods


52. Microbes in Water: Multiple-Tube Technique

53. Microbes in Water: Membrane Filter Technique

54. Microbes in Food: Contamination

55. Microbes Used in the Production of Foods

56. Microbes in Soil: The Nitrogen and Sulfur Cycles

57. Microbes in Soil: Bioremediaton


A. Pipetting

B. Dilution Techniques and Calculations

C. Use of the Spectrophotometer

D. Graphing

E. Use of the Dissecting Membrane

F. Use of the Membrane Filter

G. Electrophoresis

H. Keys to Bacteria


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