Laboratory Experiments in Microbiology / Edition 8

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Overview

Newly revised to correspond to all current undergraduate one-semester microbiology textbooks. This lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology and is an ideal companion to Microbiology: An Introduction, Ninth Edition by Tortora, Funke, and Case.

Microscopy: Use and Care of the Microscope, Examination of Living Microorganisms; Staining Methods, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining, Structural Stains (Endospore, Capsule, Flagella), Morphologic Unknown; Cultivation of Bacteria: Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria; Microbial Metabolism: Carbohydrate Catabolism, Fermentation, Protein Catabolism, Respiration, Rapid Identification Methods; Microbial Growth: Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms; Control of Microbial Growth: Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing; Microbial Genetics: Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting, Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens; The Microbial World: Unknown Identification and Bergey's Manual, Fungi: Yeasts, Fungi: Molds, Phototrophs: Algae and Cyanobacteria, Protozoa, VIRUSES, Isolation and Titration ofBacteriophages, Plant Viruses; Interaction of Microbe and Host: Epidemiology, Koch's Postulate, IMMUNOLOGY, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique; Microorganisms and Disease: Bacteria of the Skin, Bacteria of the Respiratory Tract, Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Urogenital Tract, Identification of an Unknown from a Clinical Sample; Microbiology and the Environment: Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique, Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles, Microbes in Soil: Bioremediaton; Appendices: Pipetting, Dilution Techniques and Calculations, Use of the Spectrophotometer, Graphing, Use of the Dissecting Membrane, Use of the Membrane Filter, Electrophoresis, Keys to Bacteria.

For all readers interested in microbiology.

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Editorial Reviews

Booknews
A laboratory companion to , Sixth Edition by Tortora, Funke, and Case. The manual contains 56 class- tested exercises, including all those recommended by the Committee on Undergraduate and Graduate Education of the American Society for Microbiology. Sections cover microscopy, staining methods, cultivation of bacteria, microbial metabolism, microbial growth, control of microbial growth, microbial genetics, the microbial world, viruses, interaction of microbe and host, immunology, microorganisms and disease, and microbiology and the environment. A color photo quiz requires students to identify an unknown. Spiral wire binding. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780805382921
  • Publisher: Benjamin Cummings
  • Publication date: 3/3/2006
  • Edition description: REV
  • Edition number: 8
  • Pages: 448
  • Product dimensions: 9.36 (w) x 10.76 (h) x 1.20 (d)

Table of Contents

Introduction.

I. MICROSCOPY.

 1. Use and Care of the Microscope.

 2. Examination of Living Microorganisms.

II. STAINING METHODS.

 3. Preparation of Smears and Simple Staining.

 4. Negative Staining.

 5. Gram Staining.

 6. Acid-fast Staining.

 7. Structural Stains (Endospore, Capsule, Flagella).

 8. Morphologic Unknown.

III. CULTIVATION OF BACTERIA.

 9. Microbes in the Environment.

10. Transfer of Bacteria: Aseptic Techniques.

11. Isolation of Bacteria by Dilution Technique.

12. Special Media for Isolating Bacteria.

IV. MICROBIAL METABOLISM.

13. Carbohydrate Catabolism.

14. Fermentation.

15. Protein Catabolism, Part 1.

16. Protein Catabolism, Part 2.

17. Respiration.

18. Rapid Identification Methods.

V. MICROBIAL GROWTH.

19. Oxygen and the Growth of Bacteria.

20. Determination of a Bacterial Growth Curve: The Role of Temperature.

21. Biofilms.

VI. CONTROL OF MICROBIAL GROWTH.

22. Physical Methods of Control: Heat.

23. Physical Methods of Control: Ultraviolet Radiation.

24. Chemical Methods of Control: Disinfectants and Antiseptics.

25. Chemical Methods of Control: Antimicrobial Drugs.

26. Effectiveness of Hand Scrubbing.

VII. MICROBIAL GENETICS.

27. Regulation of Gene Expression.

28. Isolation of Bacterial Mutants.

29. Transformation of Bacteria.

30. DNA Fingerprinting.

31. Genetic Engineering.

32. Ames Test for Detecting Possible Chemical Carcinogens.

VIII. THE MICROBIAL WORLD.

33. Unknown Identification and Bergey's Manual.

34. Fungi: Yeasts.

35. Fungi: Molds.

36.Phototrophs: Algae and Cyanobacteria.

37. Protozoa.

IX. VIRUSES.

38. Isolation and Titration of Bacteriophages.

39. Plant Viruses.

X. INTERACTION OF MICROBE AND HOST.

40. Epidemiology.

41. Koch's Postulate.

XI. IMMUNOLOGY.

42. Nonspecific Resistance.

43. Blood Group Determination: Slide Agglutination.

44. Agglutination Reactions: Microtiter Agglutination.

45. ELISA Technique.

XII. MICROORGANISMS AND DISEASE.

46. Bacteria of the Skin.

47. Bacteria of the Respiratory Tract.

48. Bacteria of the Mouth.

49. Bacteria of the Gastrointestinal Tract.

50. Bacteria of the Urogenital Tract.

51. Identification of an Unknown from a Clinical Sample.

XIII. MICROBIOLOGY AND THE ENVIRONMENT.

52. Microbes in Water: Multiple-Tube Technique.

53. Microbes in Water: Membrane Filter Technique.

54. Microbes in Food: Contamination.

55. Microbes Used in the Production of Foods.

56. Microbes in Soil: The Nitrogen and Sulfur Cycles.

57. Microbes in Soil: Bioremediaton.

APPENDICES.

A. Pipetting.

B. Dilution Techniques and Calculations.

C. Use of the Spectrophotometer.

D. Graphing.

E. Use of the Dissecting Membrane.

F. Use of the Membrane Filter.

G. Electrophoresis.

H. Keys to Bacteria.

Index.

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