Laboratory Experiments in Microbiology / Edition 9

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Newly revised to accompany Microbiology: An Introduction, Seventh Edition by Tortora, Funke, and Case, this lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology. Intended as a manual of basic microbiologic techniques, this popular lab manual features applications in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. Experiments have been refined in this new edition to encourage readers to develop critical thinking skills as well as learn basic facts and technical skills. Material with direct application to clinical and commercial labs is included whenever possible, and increased emphasis is placed on lab safety. For college instructors and students.
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Editorial Reviews

A laboratory companion to , Sixth Edition by Tortora, Funke, and Case. The manual contains 56 class- tested exercises, including all those recommended by the Committee on Undergraduate and Graduate Education of the American Society for Microbiology. Sections cover microscopy, staining methods, cultivation of bacteria, microbial metabolism, microbial growth, control of microbial growth, microbial genetics, the microbial world, viruses, interaction of microbe and host, immunology, microorganisms and disease, and microbiology and the environment. A color photo quiz requires students to identify an unknown. Spiral wire binding. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

Meet the Author

Ted R. Johnson is a professor of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he teaches courses in microbiology and immunology. He has taught at St. Olaf for 34 years and previously taught at Mankato State University. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several semester abroad and interim programs. His research focuses on the immune response to virally induced cancer in various animal models.

Christine L. Case is a registered microbiologist and a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 44 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.

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Table of Contents



1. Use and Care of the Microscope.
2. Examination of Living Microorganisms.


3. Preparation of Smears and Simple Staining.
4. Negative Staining.
5. Gram Staining.
6. Acid-fast Staining.
7. Structural Stains (Endospore, Capsule, and Flagella).
8. Morphologic Unknown.


9. Microbes in the Environment.
10. Transfer of Bacteria: Aseptic Technique.
11. Isolation of Bacteria by Dilution Techniques.
12. Special Media for Isolating Bacteria.


13. Carbohydrate Catabolism.
14. Fermentation of Carbohydrates.
15. Protein Catabolism, Part 1.
16. Protein Catabolism, Part 2.
17. Respiration.
18. Rapid Identification Methods.


19. Oxygen and the Growth of Bacteria.
20. Determination of a Bacterial Growth Curve: The Role of Temperature.
21. Other Influences on Microbial Growth: Osmotic Pressure and pH.


22. Physical Methods of Control: Heat.
23.Physical Methods of Control: Ultraviolet Radiation.
24. Chemical Methods of Control: Disinfectants and Antiseptics.
25. Chemical Methods of Control: Antimicrobial Drugs.
26. Effectiveness of Hand Scrubbing.


27. Regulation of Gene Expression.
28. Isolation of Bacterial Mutants.
29. Transformation of Bacteria.
30. DNA Fingerprinting.
31. Genetic Engineering.
32. Ames Test for Detecting Possible Chemical Carcinogens.


33. Unknown Identification and Bergey's Manual.
34. Fungi: Yeasts.
35. Fungi: Molds.
36. Phototrophs: Algae and Cyanobacteria.
37. Protozoa.


38. Isolation and Titration of Bacteriophages.
39. Plant Viruses.


40. Epidemiology.
41. Koch's Postulates.


42. Nonspecific Resistance.
43. Blood Group Determination: Slide Agglutination.
44. Agglutination Reactions: Microtiter Agglutination.
45. ELISA Technique.


46. Bacteria of the Skin.
47. Bacteria of the Respiratory Tract.
48. Bacteria of the Mouth.
49. Bacteria of the Gastrointestinal Tract.
50. Bacteria of the Urogenital Tract.
51. Identification of an Unknown from a Clinical Sample.


52. Microbes in Water: Multiple-Tube Technique.
53. Microbes in Water: Membrane Filter Technique.
54. Microbes in Food: Contamination.
55. Microbes Used in the Production of Foods.
56. Microbes in the Soil: The Nitrogen and Sulfur Cycles.
57. Microbes in Soil: Bioremediation.


A. Pipetting.
B. Dilution Techniques and Calculations.
C. Use of the Spectrophotometer.
D. Graphing.
E. Use of the Dissecting Microscope.
F. Use of the Membrane Filter.
G. Electrophoresis.
H. Keys to Bacteria.
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