Laboratory Guide for Identification of Plant Pathogenic Bacteria

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This guide is written to assist in the identification of plant pathogenic bacteria. Following the guide, most pathogens isolated from a diseased plant can be identified to the species or pathovar level by a few simple phenotypic tests. Most chapters also include material on molecular, serological, and automated commercial techniques for identification, and there are also appendices on these techniques as well. For this third edition, many nomenclatural changes have been made, and additional color photographs have...
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More About This Book

Overview

This guide is written to assist in the identification of plant pathogenic bacteria. Following the guide, most pathogens isolated from a diseased plant can be identified to the species or pathovar level by a few simple phenotypic tests. Most chapters also include material on molecular, serological, and automated commercial techniques for identification, and there are also appendices on these techniques as well. For this third edition, many nomenclatural changes have been made, and additional color photographs have been provided. The editors are affiliated with the Bacteriology Committee of The American Phytopathological Society. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780890542637
  • Publisher: American Phytopathological Society
  • Publication date: 12/1/2000
  • Format: Spiral Bound
  • Edition description: 3RD
  • Edition number: 3
  • Pages: 398

Table of Contents

Contributors III
Preface to the Third Edition VI
I. Initial Identification of Common Genera
A. Introduction 1
B. Isolation Techniques Using Differential and Semiselective Media 3
C. Differentiation of Commonly Isolated Genera 6
D. Diagnostic Media and Tests 7
E. Literature Cited 14
F. Chemicals List 15
II. Gram-Negative Bacteria
A. Agrobacterium
1. Introduction 17
2. Isolation Techniques Using Semiselective Media 18
3. Differentiation of Commonly Isolated Species 22
4. Diagnostic Media and Tests 24
5. Pathogenicity Tests 26
6. Molecular, Serological, and Commercial Automated Techniques 28
7. Culture Preservation 30
8. Literature Cited 30
9. Chemicals List 34
B. Erwinia and Pantoea
1. Introduction 36
2. Literature Cited 37
B-1. Erwinia amylovora Group
1. Introduction 40
2. Isolation Techniques Using Differential and Semiselective Media 40
3. Differentiation of Commonly Isolated Species 44
4. Diagnostic Media and Tests 44
5. Pathogenicity Tests 48
6. Molecular, Serological, and Commercial Automated Techniques 49
7. Culture Preservation 51
8. Literature Cited 51
9. Chemicals List 54
B-2. Erwinia Soft Rot Group
1. Introduction 56
2. Isolation Techniques Using Semiselective Media 56
3. Differentiation of Commonly Isolated Species and Subspecies 58
4. Diagnostic Media and Tests 59
5. Pathogenicity Tests 62
6. Molecular, Serological, and Commercial Automated Techniques 63
7. Culture Preservation 65
8. Literature Cited 66
9. Chemicals List 71
B-3. Pantoea
1. Introduction 73
2. Isolation Techniques Using Differential and Semiselective Media 73
3. Differentiation of Commonly Isolated Species 76
4. Diagnostic Media and Tests 76
5. Pathogenicity Tests 78
6. Molecular, Serological, and Commercial Automated Techniques 79
7. Culture Preservation 80
8. Literature Cited 81
9. Chemicals List 83
C. Pseudomonas
1. Introduction 84
2. Isolation Techniques Using Differential and Semiselective Media 85
3. Differentiation of Commonly Isolated Species and Pathovars 90
4. Diagnostic Media and Tests 93
5. Pathogenicity Tests 102
6. Molecular, Serological, and Commercial Automated Techniques 104
7. Culture Preservation 108
8. Literature Cited 111
9. Chemicals List 117
D. Acidovorax and Xylophilus
1. Introduction 121
2. Isolation Techniques Using Differential and Semiselective Media 122
3. Differentiation of Commonly Isolated Species and Subspecies 126
4. Diagnostic Media and Tests 128
5. Pathogenicity Tests 132
6. Molecular, Serological, and Commercial Automated Techniques 133
7. Culture Preservation 134
8. Literature Cited 135
9. Chemicals List 137
E. Burkholderia
1. Introduction 139
2. Isolation Techniques Using Differential and Semiselective Media 140
3. Differentiation of Commonly Isolated Species 142
4. Diagnostic Media and Tests 142
5. Pathogenicity Tests 145
6. Molecular, Serological, and Commercial Automated Techniques 145
7. Culture Preservation 147
8. Literature Cited 147
9. Chemicals List 150
F. Ralstonia
1. Introduction 151
2. Isolation Techniques Using Differential and Semiselective Media 153
3. Differentiation of Commonly Isolated Species and Subspecies 155
4. Diagnostic Media and Tests 157
5. Pathogenicity Tests 162
6. Molecular, Serological, and Commercial Automated Techniques 163
7. Culture Preservation 166
8. Literature Cited 167
9. Chemicals List 173
G. Xanthomonas
1. Introduction 175
2. Identification of Xanthomonadin Pigment 177
3. Isolation Techniques Using Differential and Semiselective Media 178
4. Differentiation of Commonly Isolated Species and Pathovars 188
5. Diagnostic Media and Tests 189
6. Pathogenicity Tests 190
7. Molecular, Serological, and Commercial Automated Techniques 191
8. Culture Preservation 193
9. Literature Cited 194
10. Chemicals List 199
H. Xylella fastidiosa
1. Introduction 201
2. Isolation Techniques Using Differential Media 201
3. Differentiation of Commonly Isolated Pathotypes 203
4. Diagnostic Media and Tests 204
5. Pathogenicity Tests 207
6. Molecular, Serological, and Commercial Automated Techniques 208
7. Culture Preservation 210
8. Literature Cited 210
9. Chemicals List 213
I. Rhizomonas suberifaciens
1. Introduction 214
2. Isolation Technique Using Semiselective Medium 214
3. Differentiation of Commonly Isolated Species 215
4. Diagnostic Media and Tests 215
5. Pathogenicity Tests 215
6. Culture Preservation 216
7. Literature Cited 216
8. Chemicals List 217
III. Gram-Positive Bacteria
A. Coryneform Plant Pathogens
1. Introduction 218
2. Isolation Techniques Using Semiselective Media 220
3. Differentiation of Commonly Isolated Species and Subspecies 223
4. Diagnostic Media and Tests 225
5. Pathogenicity Tests 226
6. Molecular, Serological, and Commercial Automated Techniques 227
7. Culture Preservation 229
8. Literature Cited 230
9. Chemicals List 234
B. Streptomyces
1. Introduction 236
2. Isolation Techniques Using Differential and Semiselective Media 236
3. Differentiation of Commonly Isolated Species 238
4. Diagnostic Media and Tests 240
5. Pathogenicity Tests 243
6. Molecular Techniques 245
7. Culture Preservation 245
8. Literature Cited 246
9. Chemicals List 249
C. Bacillus
1. Introduction 250
2. Isolation Techniques Using Differential Media 251
3. Differentiation of Commonly Isolated Species 252
4. Diagnostic Media and Tests 253
5. Pathogenicity Tests 256
6. Commercial Automated Techniques 256
7. Culture Preservation 256
8. Literature Cited 257
9. Chemicals List 259
D. Clostridium
1. Introduction 261
2. Isolation Techniques Using Differential and Semiselective Media 262
3. Differentiation of Commonly Isolated Species 265
4. Diagnostic Media and Tests 268
5. Inoculation Tests for Maceration of Tissue 270
6. Literature Cited 270
7. Chemicals List 274
IV. Fastidious Phloem-Limited Bacteria
A. Introduction 275
B. Isolation Techniques Using Selective Agar Media 276
C. Differentiation of Commonly Isolated Species 276
D. Diagnostic Media and Tests 277
E. Pathogenicity Tests 277
F. Molecular, Serological, and Commercial Automated Techniques 278
G. Culture Preservation 278
H. Literature Cited 279
V. Cell-Wall Free Bacteria
A. Introduction 283
B. Isolation Techniques 285
C. Differentiation of Species or Groups 293
D. Diagnostic Tests 296
E. Pathogenicity Tests 299
F. Molecular, Serological, and Commercial Automated Techniques 303
G. Literature Cited 311
H. Chemicals List 320
VI. Appendix
A. Molecular Techniques
1. Introduction 321
2. DNA Extraction 322
3. PCR-Based Methods 323
4. PER-PCR Genomic Fingerprint Analysis to Characterize and Identify Phytobacteria 325
5. Literature Cited 328
6. Diagnostic Companies 333
A-1. DNA Isolation Procedure
1. Introduction 334
B. Serological Techniques
1. Immunodiagnostic Methods 338
2. Literature Cited 339
3. Diagnostic Companies 341
C. Automated Techniques
1. Physiological and Biochemical Methods 343
2. Molecular Methods 347
3. Commercial Laboratories 350
4. Literature Cited 350
5. Diagnostic Companies 356
VII. Index 359
Color plates 260
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