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Doody's Review ServiceReviewer: Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description: This is a compilation of LC-MS/MS technical procedures for drug analysis.
Purpose: It is intended to "provide detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance."
Audience: It is written for practicing toxicologists or clinical chemists whose daily practice includes analysis for these clinically important substances. It also would be of interest to anyone curious about clinical laboratory testing for any of these compounds or interested in clinical applications of LC-MS/MS.
Features: This deceptively thin book is a real treasure trove. Aside from the first two introductory chapters, the remaining 17 are detailed protocols for LC-MS/MS analysis for various compounds. This book is not for the faint of heart — you must be a dedicated chemist/toxicologist to see the true value. The protocols in each chapter define high complexity testing. For those accustomed to load and walk away automated testing instruments, these protocols will take you back to laboratory science training or undergraduate chemistry lab. You'll need to be able to prepare all solutions from scratch — i.e., be competent in preparing analytical grade stock solutions, reagent or specimen dilutions, adjusting pHs, preparing solvents for the various phases, etc. A significant portion is devoted to substance abuse compounds (benzodiazepines, opiates, cocaine, cannabinoids, amphetamines, etc.). There are also protocols for infrequent but clinically important situations, such as hypoglycemic agents, rodenticide anticoagulants, immunosuppressive drugs, antipsychotics, etc. The major strengths of this book are the incredibly detailed protocols and helpful notes. The only minor weaknesses are relatively small chromatograph examples in certain chapters (e.g., figure 1, chapter 9) and the plethora of acronyms.
Assessment: You will need this book if you are involved in LC-MS/MS analysis for compounds of clinical interest. Having these varied and detailed protocols in a single book is priceless.