Lectins: Methods and Protocols

by Jun Hirabayashi

Overview

Lectins: Methods and Prools summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and hishemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lectins: Methods and Prools seeks to aid not only lectin specialists but also non-experts including both young scientists.

Product Details

ISBN-13:	9781493912919
Publisher:	Springer New York
Publication date:	08/13/2014
Series:	Methods in Molecular Biology , #1200
Edition description:	2014
Pages:	613
Product dimensions:	7.01(w) x 10.00(h) x 0.05(d)

Table of Contents

PART I: STANDARD TECHNIQUES

1.         History of Plant Lectin Research

Els J.M. Van Damme

2.         Fungal Lectins, A Growing Family

Yuka Kobayashi, and Hirokazu Kawagishi

3.         The “White Kidney Bean Incident” in Japan

Haruko Ogawa, and Kimie Date

4.         Hemagglutination (Inhibition) Assay

Kotone Sano, and Haruko Ogawa

5.         Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources

Kimie Date and Haruko Ogawa

6.         High-Performance Lectin Affinity Chromatography

Yuka Kobayashi

7.         Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography

Katsuko Yamashita, and Takashi Ohkura

8.         Lectin-Probed Western Blot Analysis

Takeshi Sato

9.         Solid-Phase Assay of Lectin Activity Using HRP-conjugated Glycoproteins

Kyoko Kojima-Aikawa

10. A Simple Viral Neuraminidase-based Detection for High-throughput Screening of Viral Hemagglutinin-host Receptor Specificity

Nongluk Sriwilaijaroen, and Yasuo Suzuki   

11.     Lectin Affinity Electrophoresis

Yuka Kobayashi

12.     Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis between Lectins and Glycans

Mitsuhiro Kinoshita, and Kazuaki Kakehi

13.     Basic Procedures for Lectin Flow Cytometry

Kenta Moriwaki, and Eiji Miyoshi

14.     Hishemical Staining Using Lectin Probes

Yoshihiro Akimoto, and Hayato Kawakami

15.     Equilibrium Dialysis using Chromophoric Sugar Derivatives

Tomomitsu Hatakeyama

16.     Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis between Lectins and Sugars

Kanji Hori and Makoto Hirayama

17.     Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions

Yasuro Shinohara, and Jun-ichi Furukawa

18.     Isothermal Calorimetric Analysis of Lectin–sugar Interaction

Yoichi Takeda and Ichiro Matsuo

19.     Carbohydrate–Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines

Mamoru Mizuno

PART II: EMERGING TECHNIQUES FOR LECTIN-BASED GLYCOMICS

20.     Lectin-Based Glycomics: How and When Was the Technology Born?

Jun Hirabayashi

21.     Frontal Affinity Chromatography (FAC): Theory and Basic Aspects

Ken-ichi Kasai

22.     Frontal Affinity Chromatography: Practice of Weak Interaction Analysis Between Lectins and Fluorescently Labeled Oligosaccharides

Chihiro Sato

23.     Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation. From Sample Pretreatment to Data Standardization

Atsushi Kuno, Atsushi Matsuda, Sachiko Unno, Binbin Tan, Jun Hirabayashi, and Hisashi Narimatsu

24.     Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces

Azusa Shibazaki, and Tohru Gonoi

25.      Application of Lectin Microarray to Bacteria Including Lactobacillus casei /paracasei Strains

Emi Yasuda, Tomoyuki Sako, Hiroaki Tateno, and Jun Hirabayashi

26.     Live Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN

Hiroaki Tateno, Yasuko Onuma, and Yuzuru Ito

27.     Carbohydrate-binding Specificity of Lectins Using Multiplexed Glyco-bead Array

Kazuo Yamamoto

28.     Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins

Yu-ki Matsuno, and Akihiko Kameyama

PAPRT III: TECHNIQUES FOR ELUCIDATING FUNCTIONS OF ENDOGENOUS ANIMAL LECTINS

29.     Overall Strategy for Functional Analysis of Animal Lectins

Norihito Kawasaki

30.     Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System

Hiroaki Tateno

31.     Potential Usage for in vivo Lectin Screening in Live Animals Utilized by Cell Surface Mimetic Glyco-nanoparticles, Phosphorylcholine-Coated Quantum Dots (PC-QDs)

Maho Amano, Hiroshi Hinou, Risho Miyoshi, and Shin-Ichiro Nishimura

32.     Remodeling Cell Surface Glycans using Glycosylation Inhibitors

Norihito Kawasaki

33.     Remodeling of Glycans Using Glycosyltransferase Genes

Yuko Naito-Matsui, and Hiromu Takematsu

34.     Functional Assay Using Lectin Gene Targeting Technologies (Over-Expression)

Motohiro Nonaka, and Toshisuke Kawasaki

35.     Analysis of L-selectin-mediated Cellular Interactions under Flow Conditions

Hiroto Kawashima

36.     Assessment of weak sugar-binding ability using lectin tetramer and membrane-based glycans

Kazuo Yamamoto

PART IV: STRUCTURAL BIOLOGY AND ENGINEERING OF LECTINS

37.     Perspectives in Glycomics and Lectin Engineering

Jan Tkac, Tomas Bertok, Jozef Nahalka, and Peter Gemeiner

38.     Molecular Basis of Pandemic of Avian-Type Influenza Virus

Nongluk Sriwilaijaroen, and Yasuo Suzuki

39.     Basic Procedure of X-ray Crystallography for Analysis of Lectin–sugar Interactions

Zui Fujimoto

40.     A New Structure Determination Method of Lectins Using a Selenium-containing Sugar Ligand

Hisayoshi Makyio, and Ryuichi Kato

41.     NMR Analysis of Carbohydrate-Binding Interactions in Solution: An Approach Using Analysis of Saturation Transfer Difference NMR Spectroscopy

Hikaru Hemmi

42.     Small Angle X-ray Scattering to Obtain Models of Multivalent Lectin-Glycan Complexes

Stephen D. Weeks, and Julie Bouckaert

43.     Directed Evolution of Lectins by an Improved Error-prone PCR and Ribosome Display Method

Dan Hu, Hiroaki Tateno, and Jun Hirabayashi

44.     Tracing Ancestral Specificity of Lectins: Ancestral Sequence Reconstruction Method as a New Approach in Protein Engineering

Tomohisa Ogawa, and Tsuyoshi Shirai

PART V: COMPREHENSIVE LISTS

45.       Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities

Yuka Kobayashi, Hiroaki Tateno, Haruko Okawa, Kazuo Yamamoto, and Jun Hirabayashi

46.     Lectin Structures: Classification Based on the 3-D Structures

Zui Fujimoto, Hiroaki Tateno, and Jun Hirabayashi