Life Illuminated: Selected Papers from Cold Spring Harbor Volume 2, 1972-1994


This book is the second volume of an intellectual history of the science done at CSHL (the first volume, Illuminating Life, showed that genetics became the dominant theme of research at CSH by as early as 1904). The appointment of James Watson as Director of the Laboratory in 1968 set off the explosive research development at CSH, as he recruited widely and wisely teams of investigators with diverse scientific interests. From this collection of papers, presented in full on the accompanying CD, several themes ...
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This book is the second volume of an intellectual history of the science done at CSHL (the first volume, Illuminating Life, showed that genetics became the dominant theme of research at CSH by as early as 1904). The appointment of James Watson as Director of the Laboratory in 1968 set off the explosive research development at CSH, as he recruited widely and wisely teams of investigators with diverse scientific interests. From this collection of papers, presented in full on the accompanying CD, several themes emerge: the characterization and exploitation of mobile genetic elements; the mechanics of DNA replication and regulation of the cell cycle; the behavior and internal architecture of cells; how viruses induce tumors; the discovery of cancer genes; the characteristics of neurons; and the invention of techniques that make possible further progress. Each theme is introduced in the context of the science of the time, and each paper has a commentary by, in most cases, one of its authors. Life Illuminated is a story of scientific innovation and achievement, told in the words of the investigators themselves.
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Editorial Reviews

The Quarterly Review of Biology
Perhaps most importantly, this is a volume about life at Cold Spring Harbor. Very few pages pass without a mention of some word or deed by James Watson and the presence of Barbara McClintock is felt throughout the book. There are fascinating descriptions of Watson making his nightly rounds of the research laboratories, and of him wryly reminding a postdoctoral researcher caught building a model of DNA: '[t]hat's been done'. Tim Tully tells an interesting tale of Watson's willingness to support a long and often frustrating assault on the biology of memory.
In an early chapter, Bruce Alberts reminds us that 'cells are much smarter than scientists'. True enough, but the denizens of CSHL in the 1970s and 1980s were (and still are) rapidly closing the gap.
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Product Details

  • ISBN-13: 9780879698041
  • Publisher: Cold Spring Harbor Laboratory Press
  • Publication date: 5/1/2008
  • Pages: 208
  • Product dimensions: 6.70 (w) x 9.10 (h) x 1.10 (d)

Table of Contents

Preface   Jan A. Witkowski   Alexander Gann   Joseph F. Sambrook     xi
Acknowledgments     xv
Introduction: Genes Are Interesting to Geneticists   Gerald R. Fink     1
Ljungquist E. and Bukhari A.I. 1977. State of prophage Mu DNA upon induction. Proc. Natl. Acad. Sci. 74: 3143-3147   Rasika Harshey     7
Taylor A.L. and Szybalski W. 1984. In memoriam: Ahmad I. Bukhari, 1943-1983. Gene 27: iii-iv   Bukhari Memoriam     12
Strathern J.N, Klar A.J.S., Hicks J.B., Abraham J.A., Ivy J.M., Nasmyth K.A., and McGill C. 1982. Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus. Cell 31: 183-192   James B. Hicks     13
Sundaresan V., Springer P., Volpe T., Haward S., Jones J.D.G., Dean C., Ma H., and Martienssen R. 1995. Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes Dev. 9: 1797-1810   Robert Martienssen     19
Draetta G., Luca F., Westendorf J., Brizuela L., Ruderman J., and Beach D. 1989. cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF. Cell 56: 829-838   David Beach     25
Introduction: Why the Revolution That Jim Started Continues   Bruce Alberts     31
Watson J.D. 1972. Origin of concatemeric T7 DNA.Nat. New Biol. 239: 197-201   Joseph F. Sambrook     37
Prelich G., Kostura M., Marshak D.R., Mathews M.B., and Stillman B. 1987. The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro. Nature 326: 471-475   Bruce Stillman     41
Bell S.P. and Stillman B. 1992. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature 357: 128-134   Bruce Stillman     47
Klimasauskas S., Kumar S., Roberts R.J., and Cheng X. 1994. Hhal methyltransferase flips its target base out of the DNA helix. Cell 76: 357-369   Richard J. Roberts     51
Cell Biology
Introduction: From Architecture to Functional Analysis   David L. Spector     55
Lazarides E. and Weber K. 1974. Actin antibody: The specific visualization of actin filaments in non-muscle cells. Proc. Natl. Acad. Sci. 71: 2268-2272   Klaus Weber     61
Albrecht-Buehler G. 1977. Daughter 3T3 cells. Are they mirror images of each other? J. Cell Biol. 72: 595-603   Guenter Albrecht-Buehler     65
Bar-Sagi D. and Feramisco J.R. 1985. Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. Cell 42: 841-848   Dafna Bar-Sagi   James R. Feramisco     69
Harley C.B., Futcher A.B., and Greider C.W. 1990. Telomeres shorten during ageing of human fibroblasts. Nature 345: 458-460    Carol W. Greider     75
Spector D.L. 1990. Higher order nuclear organiation: Three-dimensional distribution of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. 87: 147-151   David L. Spector     83
Tumor Viruses
Introduction: The Early Days of Tumor Virus Research at Cold Spring Harbor   Joseph F. Sambrook     89
Sambrook J., Sharp P.A., and Keller W. 1972. Transcription of simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J. Mol. Biol. 70: 57-71   Joseph F. Sambrook     95
Grodzicker T., Williams J., Sharp P., and Sambrook J. 1974. Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harbor Symp. Quant. Biol. 39(Pt 1): 439-446   Terri Grodzicker     99
Chow L.T., Gelinas R.E., Broker T.R., and Roberts R.J. 1977. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA. Cell 12: 1-8   Louise T. Chow     105
Botchan M., Topp W., and Sambrook J. 1976. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell 9: 269-287   Michael Botchan     113
Tjian R. 1978. The binding site on SV40 DNA for a T antigen-related protein. Cell 13: 165-179   Robert Tjian     119
Gluzman Y. 1981. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182   Joseph F. Sambrook     125
Thummel C., Tjian R., and Grodzicker T. 1981. Expression of SV40 T antigen under control of adenovirus promoters. Cell 23: 825-836   Carl S. Thummel     129
Herr W. and Clarke J. 1986. The SV40 enhancer is composed of multiple functional elements that can compensate for one another. Cell 45: 461-470   Winship Herr     133
Introduction: Neuroscience at CSH Laboratory: Meetings, Courses, and Research   Eric Kandel     139
Introduction: Getting the Point at Cold Spring Harbor   Ron McKay     145
Zipser B. and McKay R. 1981. Monoclonal antibodies distinguish identifiable neurones in the leech. Nature 289: 549-554   Birgit Zipser     151
Hockfield S. and McKay R.D.G. 1985. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5: 3310-3328   Ron McKay     157
Yin J.C.P., Wallach J.S., Del Vecchio M., Wilder E.L., Zhou H., Quinn W.G., and tully T. 1994. Induction of a dominant negative CREB transgene specifically blocks long-term memory in Drosophila. Cell 79: 49-58   Tim Tully     163
Introduction: The Road to Understanding the Origins of Cancer in Humans   Arnold J. Levine     169
Goldfarb M., Shimizu K., Perucho M., and Wigler M. 1982. Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells. Nature 296: 404-409   Mitchell Goldfarb     175
Ruley H.E. 1983. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature 304: 602-606   Earl Ruley     181
Kataoka T., Powers S., Cameron S., Fasano O., Goldfarb M., Broach J., and Wigler M. 1985. Functional homology of mammalian and yeast RAS genes. Cell 40: 19-26   Scott Powers     187
Hanahan D. 1985. Heritable formation of pancreatic [beta]-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes. Nature 315: 115-122   Douglas Hanahan     193
Franza B.R. Jr., Rauscher F.J. 3rd, Josephs S.F., and Curran T. 1988. The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites. Science 239: 1150-1153   B. Robert Franza, Jr.     199
Whyte P., Buchkovich K.J., Horowitz J.M., Friend S.H., Raybuck M., Weinberg R.A., and Harlow E. 1988. Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. Nature 334: 124-129   Ed Harlow     203
Introduction: Early Technological Breakthroughs at Cold Spring Harbor   Angela N.H. Creager     209
Roberts R.J. 1978. Restriction and modification enzymes and their recognition sequences. Gene 4: 183-194   Richard J. Roberts     215
Garrels J.I. 1989. The QUEST system for quantitative analysis of two-dimensional gels. J. Biol. Chem. 264: 5269-5282   James I. Garrels     219
Sharp P.A., Sugden B., and Sambrook J. 1973. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12: 3055-3063   Joseph F. Sambrook     223
Maniatis T., Kee S.G., Efstratiadis A., and Kafatos F.C. 1976. Amplification and characterization of a [beta]-globin gene synthesized in vitro. Cell 8: 163-182   Tom Maniatis     227
Author Index     235
Index     237
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