Lipid Signaling Protocols

Overview

Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing...
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Overview

Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes, and the recognition proteins. Following the highly successful Methods in Molecular Biology series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Product Details

  • ISBN-13: 9781603271158
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 1/1/2009
  • Pages: 429

Table of Contents

Part I Lipid Systems and Techniques

1 Eicosanoids: Generation and Detection in Mammalian Cells Valerie B. O'Donnell Ben Maskrey Graham W. Taylor 5

2 Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry Trevor R. Pettitt 25

3 Measurement of Polyphosphoinositides in Cultured Mammalian Cells Frank T. Cooke 43

4 Inositol Lipid-Dependent Functions in Saccharomyces cerevisiae: Analysis of Phosphatidylinositol Phosphates Stephen K. Dove Robert H. Michell 59

5 Methods for the Determination of the Mass of Nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P[subscript 2] David R. Jones Yvette Bultsma Willem Jan Keune Nullin Divecha 75

6 Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin Marie Garnier-Lhomme Erick J. Dufourc Banafshe Larijani Dominic Poccia 89

7 Solution and Solid-State NMR of Lipids Axelle Grelard Anthony Couvreux Cecile Loudet Erick J. Dufourc 111

8 Spatial Localization of PtdInsP[subscript 2] in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLC-delta 1 PH Domain Xavier Mulet Erika Rosivatz Ka Kei Ho Beatrice L.L.E. Gauthe Oscar Ces Richard H. Templer Rudiger Woscholski 135

9 Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes Sebastian J. Fleire Facundo D. Batista 145

10 Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes F.-Xabier Contreras Ana-Victoria Villar Alicia Alonso Felix M. Goni 155

11 Effects of Sterols on the Development and Aging of Caenorhabditis elegans Eun-Young Lee Pan-Young Jeong Sun-Young Kim Yhong-Hee Shim David J. Chitwood Young-Ki Paik 167

12Proteomic Analysis of the Sterol-Mediated Signaling Pathway in Caenorhabditis elegans Byung-Kwon Choi Yun-Kyung Shin Eun-Young Lee Pan-Young Jeong Yhong-Hee Shim David J. Chitwood Young-Ki Paik 181

Part II Lipid Metabolism and Recognition

13 Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstream Effectors of PI3K/Akt Abdelkader Essafi Ana R. Gomes Karen M. Pomeranz Aleksandra K. Zwolinska Rana Varshochi Ursula B. McGovern Eric W.-F. Lam 201

14 Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt Erika Rosivatz Rudiger Woscholski 213

15 Analysis of Phosphatidylinositol 3,4,5 Trisphosphate 5-Phosphatase Activity by in vitro and in vivo Assays Lisa M. Ooms Jennifer M. Dyson Anne M. Kong Christina A. Mitchell 223

16 Measuring Phospholipase D Activity in Insulin-Secreting Pancreatic [beta]-Cells and Insulin-Responsive Muscle Cells and Adipocytes Rosanna Cazzolli Ping Huang Shuzhi Teng William E. Hughes 241

17 Protein Kinase C as an Effector of Lipid-Derived Second Messengers Marie-Helene Paclet Jan K. Davidson-Moncada Guillermo Lopez-Lluch Dongmin Shao Lodewijk V. Dekker 253

18 Detection of Myotubularin Phosphatases Activity on Phosphoinositides in vitro and ex vivo Holger Maria Rohde Helene Tronchere Bernard Payrastre Jocelyn Laporte 265

19 Quantification of Multiple Phosphatidylinositol 4-Kinase Isozyme Activities in Cell Extracts Mark G. Waugh Shane Minogue J. Justin Hsuan 279

20 Phospholipid-Interacting Proteins by Solution-State NMR Spectroscopy Keiichiro Kami Sundaresan Rajesh Michael Overduin 291

21 Revealing Signaling in Single Cells by Single- and Two-Photon Fluorescence Lifetime Imaging Microscopy Damien Alcor Veronique Calleja Banafshe Larijani 307

22 Phosphoinositide (PI) 3-Kinase Assays Michael J. Fry 345

23 Measurement of Phosphatidylinositol and Phosphatidylcholine Binding and Transfer Activity of the Lipid Transport Protein PITP Shamshad Cockcroft 363

24 In vitro Reconstitution of Activation of PLC[epsion] by Ras and Rho GTPases Natalia Lamuno Gandarillas Tom D. Bunney Michelle B. Josephs Peter Gierschik Matilda Katan 379

25 Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities Jonathon R. Halstead Mireille H. Snel Sarah Meeuws David R. Jones Nullin Divecha 391

26 Preparation of Membrane Rafts Mark G. Waugh J. Justin Hsuan 403

Index 415

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