The Logic of Biochemical Sequencing / Edition 1

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Overview

The Logic of Biochemical Sequencing examines how to determine the primary structures of proteins and DNA and use them to stimulate the process of logical problem-solving. It concentrates on sequencing work and stresses the thought processes needed to make sense of what might otherwise be indecipherable data. The book also introduces "biocryptography," which serves as a basis for four short stories that use the results of sequence determinations to provide clues to higher order problems. Problems in the book range from elementary to difficult, and solutions to all problems are provided, many of them completely worked out. The book is an excellent supplementary text for students in a full-year biochemistry course, as well as for biochemists and molecular biologists.

This book contains black-and-white illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Gene A. Homandberg, PhD (Rush Medical College of Rush University)
Description: This monograph presents sequencing data and sequencing problems and discusses the logic of sequencing. Both protein and DNA sequencing data are presented and discussed, with problems presented at the end of each chapter and in a separate chapter. This book is unique because of a chapter on the adventures in sequencing, a highly imaginative story about a CIA cryptanalyst who discovers that the Russians are using peptide sequence and restriction data to encode messages. The sequencing logic usedto decode the messages is presented in a dialog between two characters, in a highly imaginative and effective fashion.
Purpose: The purpose is to present the logic of protein and DNA sequencing and also to teach that the approach used in sequencing involves common logic.
Audience: The audience is meant to be structural biochemists with interests in sequencing. Molecular biologists will also find this book interesting. The audience should include students in biomedical sciences.
Features: The book's tables and line drawings are of sufficient quality; however, it should be noted that there are no illustrations for those with no background in sequencing. This book is aimed at individuals with some basic knowledge of the chemistry and data acquisition methods. Unfortunately, there are no literature references for these basics or for additional learning. However, the objectives are still very worthy, and the book is very successful in its stated purpose.
Assessment: This book has the unique objective of focusing on the logic of analyzing sequencing data, and it instills fun in this learning objective by including a chapter on adventures in sequencing. The combination of protein and DNA sequencing approaches is important, useful, and very effective. The problems are well thought out. This book is highly recommended for the library, bookstore, biochemist, and especially the graduate student.

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780849344978
  • Publisher: CRC Press
  • Publication date: 10/25/1993
  • Edition number: 1
  • Pages: 192
  • Product dimensions: 5.99 (w) x 9.27 (h) x 0.41 (d)

Table of Contents

1 End Group Analysis of Proteins
Reactions of the Amino Group 2
Reactions of the Carboxyl Group 5
2 Limited Hydrolysis of Proteins
Cyanogen Bromide 11
Trypsin 16
Chymotrypsin 22
Elastase 24
Pepsin 24
3 Base Sequencing of DNA
The Maxam-Gilbert Method 34
The Sanger Method 37
4 Analysis of DNA by Restriction Mapping
Single Enzyme Digests 43
Multiple Enzyme Digests 49
5 Additional Problems 61
6 Adventures in Sequencing
Biocryptography - A New Science 73
A Night at the Opera (House) 82
From Baltimore with Love 90
Summertime, and Is the Living Easy? 117
Appendix A Solutions to End of Chapter Problems 129
Appendix B Answers to Additional Problems 155
Index 159
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