Gift Guide

Luminal calcium regulates membrane trafficking in the early secretory pathway.

More About This Textbook


Calcium is an important regulatory ion which acts as a trigger or is required at a basal level for many membrane fusion events. The role of calcium, and in particular luminal calcium, in regulating fusion in the early secretory pathway has not been thoroughly investigated. Here, I show that calcium has an inhibitory effect on homotypic coat protein complex (COPII) vesicle fusion. In particular, chelation of luminal calcium as well as fast chelation of extra luminal calcium, result in a significant increase of COPII vesicle fusion. Specific inhibition of Sarco/endoplasmic reticulum calcium ATPases using cyclopiazonic acid in the presence of calcium free medium results in low luminal calcium while maintaining normal calcium concentration in the cytosol. This luminal calcium depletion results in a dramatic expansion of the endoplasmic reticulum to Golgi intermediate compartment (ERGIC) in the periphery of the cell visualized using the markers rbet1 and p24. Depletion of luminal calcium also results in major kinetic defects of VSV-G transport to the Golgi, as well as reconstitution of the Golgi after it has been dispersed using brefeldin A. These results suggest that luminal calcium regulates fusogenicity, structure, and function of COPII derived membranes in transport. However, bulk soluble cargo is not affected by luminal calcium depletion. This suggests that luminal calcium may act at the level of membrane protein sorting in the ERGIC. Possible mechanisms by which luminal calcium exerts these novel roles are discussed herein and include prolonged retention of the outer COPII shell Sec13/Sec31 with Apoptosis Linked Gene-2 (ALG-2) being a likely candidate as the calcium sensor on the outside of the vesicle.
Read More Show Less

Product Details

  • BN ID: 2940043544865
  • Publisher: ProQuest LLC
  • Sold by: Barnes & Noble
  • Format: eTextbook
  • Pages: 110

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)