Making and Using Antibodies: A Practical Handbook

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2006 Plastic Comb (Softcover) Good Plastic-comb binding. Creasing and wear to covers. Some crease/bent page edges. 0849335280.

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Overview

Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.

Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-

  • Commercially available adjuvants designed for the production of antisera in the research setting
  • Methods for the production, purification, and characterization of antibodies
  • Practical guidance to researchers needing to modify antibodies
  • Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry
  • Methods for applying immunolabeling to the realm of electron microscopy (EM)
  • New antibody sources and new ways to use antobodies

Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.

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Product Details

  • ISBN-13: 9780849335280
  • Publisher: CRC Press
  • Publication date: 8/15/2006
  • Pages: 408
  • Product dimensions: 6.60 (w) x 9.00 (h) x 1.00 (d)

Table of Contents

Antibodies; Matthew R. Kaser and Gary C. Howard

Antigens; Paul Algate, Jory Baldridge, and Sally Mossman

Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman

Production of Polyclonal Antibodies; Lon V. Kendall

Production of Monoclonal Antibodies; Kathleen C. F. Sheehan

Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith

Purification and Characterization of Antibodies; Joseph P.
Chandler

Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu

Chemical and Proteolytic Modification of Antibodies; George P. Smith

Applications; Lee Bendickson and Marit Nilsen-Hamilton

Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele

Immunoelectron Microscopy; Sara E. Miller and David N. Howell

Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze

ELISAs; John Chen and Gary C. Howard

Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard

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