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Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.
Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-
Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.
Antibodies; Matthew R. Kaser and Gary C. Howard
Antigens; Paul Algate, Jory Baldridge, and Sally Mossman
Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman
Production of Polyclonal Antibodies; Lon V. Kendall
Production of Monoclonal Antibodies; Kathleen C. F. Sheehan
Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith
Purification and Characterization of Antibodies; Joseph P.
Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu
Chemical and Proteolytic Modification of Antibodies; George P. Smith
Applications; Lee Bendickson and Marit Nilsen-Hamilton
Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele
Immunoelectron Microscopy; Sara E. Miller and David N. Howell
Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze
ELISAs; John Chen and Gary C. Howard
Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard