Mammalian Oocyte Regulation: Methods and Protocols

Overview

Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg) capable of fertilisation and embryonic development is of unrivalled complexity. The end-product of this remarkable developmental process is the "mature" egg, now replete with macromolecules and organelles, ...

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Overview

Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg) capable of fertilisation and embryonic development is of unrivalled complexity. The end-product of this remarkable developmental process is the "mature" egg, now replete with macromolecules and organelles, and poised to support embryonic development if fertilisation should occur. Given the importance of the oocyte for embryonic development, it is of no surprise that the oocyte holds centre stage in fertility clinics and greatly influences the outcome of assisted reproductive treatments (ARTs). Mammalian Oocyte Regulation: Methods and Protocols provides a highly diverse compendium of detailed methodologies - primarily focusing on the murine model but also including chapters on human oocytes - for enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by- step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Mammalian Oocyte Regulation: Methods and Protocols serves as an ideal guide to novice oocyte researchers, experts who wish to add another dimension to their established portfolio, as well as the wider research community.

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Product Details

  • ISBN-13: 9781627031905
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/30/2012
  • Series: Methods in Molecular Biology Series, #957
  • Edition description: 2013
  • Edition number: 1
  • Pages: 311
  • Product dimensions: 7.14 (w) x 10.34 (h) x 0.91 (d)

Table of Contents

1. Studying Recombination in Mouse Oocytes
Xianfei Sun and Paula E. Cohen

2. Analysis of Recombinants in Female Mouse Meiosis
Esther de Boer, Maria Jasin, and Scott Keeney

3. Studying Meiosis Specific Cohesins in Mouse Embryonic Oocytes
Ji-hye Kim, Kei-ichiro Ishiguro, Nobuaki Kudo, and Yoshinori Watanabe

4. Immunohishemical Approaches to the Study of Human Fetal Ovarian Development
Jing He, Andrew J. Childs, Jieqian Zhou, and Richard A. Anderson

5. Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos
Jacek Z. Kubiak

6. Time-Lapse Epi-Fluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes
Janet E. Holt, Simon I.R. Lane, and Keith T. Jones

7. Using FRET to Study RanGTP Gradients in Live Mouse Oocytes
Julien Dumont and Marie-Hélène Verlhac

8. Making cRNA for Microinjection and Expression of Fluorescently-Tagged Proteins for Live-Cell Imaging in Oocytes
Mark Levasseur

9. RNAi-Based Methods for Gene Silencing in Mouse Oocytes
Paula Stein, Petr Svoboda, and Richard M. Schultz

10. Micro-Injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes
Petros Marangos

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinehores in Human Oocytes
Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes
Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Lynne Anguish and Scott Coonrod

16. Measuring Ca2+ Oscillations in Mammalian Eggs
Karl Swann

17. Counting Chromosomes in Intact Eggs
Teresa Chiang and Michael A. Lampson

18. Free-Hand Bisection of Mouse Oocytes and Embryos
Zbigniew Polanski and Jacek Z. Kubiak

19. Microarray-CGH for the Assessment ofAneuploidy in Human Polar Bodies and Oocytes
Souraya Jaroudi and Dagan Wells

20. Nuclear Transfer in the Mouse Oocyte
Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama
Petros Marangos

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinehores in Human Oocytes
Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes
Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Lynne Anguish and Scott Coonrod

16. Measuring Ca2+ Oscillations in Mammalian Eggs
Karl Swann

17. Counting Chromosomes in Intact Eggs
Teresa Chiang and Michael A. Lampson

18. Free-Hand Bisection of Mouse Oocytes and Embryos
Zbigniew Polanski and Jacek Z. Kubiak

19. Microarray-CGH for the Assessment ofAneuploidy in Human Polar Bodies and Oocytes
Souraya Jaroudi and Dagan Wells

20. Nuclear Transfer in the Mouse Oocyte
Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama
Petros Marangos

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinehores in Human Oocytes
Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes
Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Lynne Anguish and Scott Coonrod

16. Measuring Ca2+ Oscillations in Mammalian Eggs
Karl Swann

17. Counting Chromosomes in Intact Eggs
Teresa Chiang and Michael A. Lampson

18. Free-Hand Bisection of Mouse Oocytes and Embryos
Zbigniew Polanski and Jacek Z. Kubiak

19. Microarray-CGH for the Assessment ofAneuploidy in Human Polar Bodies and Oocytes
Souraya Jaroudi and Dagan Wells

20. Nuclear Transfer in the Mouse Oocyte
Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama
Petros Marangos

11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
Jay M. Baltz, Hannah E. Corbett, and Samantha Richard

12. Immunofluorescence Staining of Spindles, Chromosomes and Kinehores in Human Oocytes
Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer

13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang

14. Chromosome Spreads with Centromere Staining in Mouse Oocytes
Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann

15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Lynne Anguish and Scott Coonrod

16. Measuring Ca2+ Oscillations in Mammalian Eggs
Karl Swann

17. Counting Chromosomes in Intact Eggs
Teresa Chiang and Michael A. Lampson

18. Free-Hand Bisection of Mouse Oocytes and Embryos
Zbigniew Polanski and Jacek Z. Kubiak

19. Microarray-CGH for the Assessment ofAneuploidy in Human Polar Bodies and Oocytes
Souraya Jaroudi and Dagan Wells

20. Nuclear Transfer in the Mouse Oocyte
Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama

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