Manipulating the Mouse Embryo: A Laboratory Manual / Edition 3

Paperback (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $66.60
Usually ships in 1-2 business days
(Save 60%)
Other sellers (Paperback)
  • All (5) from $66.60   
  • New (1) from $146.00   
  • Used (4) from $66.60   


Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cuttingedge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.
Read More Show Less

Product Details

  • ISBN-13: 9780879695910
  • Publisher: Cold Spring Harbor Laboratory Press
  • Publication date: 12/13/2002
  • Edition description: Quark
  • Edition number: 3
  • Pages: 776
  • Product dimensions: 8.42 (w) x 11.08 (h) x 1.24 (d)

Table of Contents

Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future
Chapter 2. Summary of Mouse Development
Chapter 3. Production of Transgenic and Chimeric Mice: General Issues
Chapter 4. Recovery and In Vitro Culture of Pre-implantation-Stage Embryos
Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos
Chapter 6. Surgical Procedures
Chapter 7. Production of Transgenic Mice
Chapter 8. Isolation, Culture, and Manipulation of Blastocyst-derived Stem Cells
Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations
Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells
Chapter 11. Production of Chimeras
Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences
Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning
Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection
Chapter 15. Cryopreservation, Rederivation, and Transport of Mice
Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems
Chapter 17. Setting up and Equipping a Micromanipulation Lab
Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)