Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition / Edition 2

Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition / Edition 2

by Mary S. Lipton
     
 

ISBN-10: 1934115487

ISBN-13: 9781934115480

Pub. Date: 12/12/2008

Publisher: Springer-Verlag New York, LLC

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. As a result of these rapid developments that have led

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Overview

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. As a result of these rapid developments that have led to expanded areas of research and innovative applications, an update to Mass Spectrometry of Proteins and Peptides is not only worthwhile, but arguably mandatory. Spanning fields from microbial forensics and clinical applications to protein structure, dynamics and function, the following chapters written by leading experts serve as a forum for presenting some of the latest revolutionizing methods for mass spectrometry-based characterization of proteins and peptides. Staying faithful to the spirit of the Methods in Molecular Biology™ series, all chapters are written in the same format, whereby each chapter opens with a basic description of the method, followed by a description of the prool and then practical step-by-step instructions to ensure experimental success.

Mass Spectrometry of Proteins and Peptides: Methods and Prools, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology.

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Product Details

ISBN-13:
9781934115480
Publisher:
Springer-Verlag New York, LLC
Publication date:
12/12/2008
Series:
Methods in Molecular Biology Series, #492
Edition description:
2nd ed. 2009
Pages:
470
Product dimensions:
7.70(w) x 10.30(h) x 1.10(d)

Table of Contents

Preface

Contributors



• Generation and Analysis of Multidimensional Protein Identification Technology Datasets

Selene K. Swanson, Laurence Florens and Michael P. Washburn
• Quantitative Peptide and Protein Profiling by Mass Spectrometry

Alexander Schmidt, Birgit Bisle and Thomas Kislinger
• Label Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach

Kim K. Hixson
• Classical Proteomics: Two-dimensional Electrophoresis/MALDI Mass Spectrometry

Ursula Zimny-Arndt, Monika Schmid, Renate Ackermann and Peter R. Jungblut
• The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression

Rajat Sapra
• Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins

Christian G. Huber, Anna-Maria Timperio, Hansjörg Toll and Lello Zolla
• High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation

Jesper V. Olsen and Boris Macek
• Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra

Amy Nichols and Forest White
• Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry

Yehia Mechref, Milan Madera and Milos V. Novotny
• Structure Analysis of N-glycoproteins

Stefanie Henning, Jasna Peter-Katalinic and Gottfried Pohlentz
• Capillary Zone Electrophoresis-mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins

Christian Neusüß and Matthias Pelzing
• Top-down Proteomics on a High-field Fourier Transform Ion Cyclotron Resonance Mass spectrometer

Séverine A. Ouvry-Patat, Matthew P. Torres, Craig Gelfand, Hung-Hiang Quek, Michael Easterling, J. Paul Speir and Christoph H. Borchers
• Capillary Isoelectric Focusing/Reversed Phase Liquid Chromatography/Mass Spectrometry

Cheng S. Lee and Brian M. Balgley
• Integrating Accelerated Tryptic Digestion into Proteomics Workflows

Gordon W. Slysz and David C. Schriemer
• Hydrogen/Deuterium Exchange Mass Spectrometry

Xuguang Yan and Claudia S. Maier
• Mass Spectrometry Detection and Characterization of Noncovalent Protein Complexes

Sheng Yin and Joseph A. Loo
• Chemical Cross-linking for Protein-protein Interaction Studies

Xiaoting Tang and James E. Bruce
• Tissue Analysis with High Resolution Imaging Mass Spectrometry

A.F. Maarten Altelaar and Ron M.A. Heeren
• Proteomic Global Profiling for Cancer Biomarker Discovery

Samir Hanash
• Analysis of Protein Glycosylation and Phosphorylation Using Liquid Phase Separation, Protein Microarray Technology and Mass Spectrometry

Jia Zhao, Tasneem H. Patwa, Manoj Pal, Weilian Qiu and David M. Lubman
• Transthyretin Mass Determination for Detection of Transthyretin Familial Amyloid

John F. O’Brien and H. Robert Bergen, III
• Characterization of Microorganisms by MALDI Mass Spectrometry

Catherine E. Petersen, Nancy B. Valentine and Karen L. Wahl
• Mass Spectrometric Characterization Of Neuropeptides

Stephanie S. DeKeyser, James A. Dowell and Lingjun Li
• Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods

Scott A. McLuckey
• Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics

Roman A. Zubarev
• Two-Dimensional Ion Mobility Analyses of Proteins and Peptides

Alexandre A. Shvartsburg, Keqi Tang and Richard D. Smith
• Proteomics for Validation of Automated Gene Model Predictions

Kemin Zhou, Ellen A. Panisko, Jon K. Magnuson, Scott E. Baker and Igor V. Grigoriev
• Support Vector Machines for Improved Peptide Identification from Tandem Mass Spectrometry Database Search


Bobbie-Jo M. Webb-Robertson

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