Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition / Edition 2

Hardcover (Print)
Buy New
Buy New from
Used and New from Other Sellers
Used and New from Other Sellers
from $70.00
Usually ships in 1-2 business days
(Save 49%)
Other sellers (Hardcover)
  • All (13) from $70.00   
  • New (9) from $102.93   
  • Used (4) from $70.00   


When the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. As a result of these rapid developments that have led to expanded areas of research and innovative applications, an update to Mass Spectrometry of Proteins and Peptides is not only worthwhile, but arguably mandatory. Spanning fields from microbial forensics and clinical applications to protein structure, dynamics and function, the following chapters written by leading experts serve as a forum for presenting some of the latest revolutionizing methods for mass spectrometry-based characterization of proteins and peptides. Staying faithful to the spirit of the Methods in Molecular Biology™ series, all chapters are written in the same format, whereby each chapter opens with a basic description of the method, followed by a description of the prool and then practical step-by-step instructions to ensure experimental success.

Mass Spectrometry of Proteins and Peptides: Methods and Prools, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology.

Read More Show Less

Editorial Reviews

From the Publisher
From the reviews of the second edition:

"This book … covers a wide range of applications of modern mass spectrometry in peptide and protein analysis. … Very useful is the presence of a final ‘Notes’ section within each chapter. These notes and laboratory tips help both beginners and experienced researchers to make their first steps into new fields … . The well balanced mixture … makes it a helpful resource for scientists working in analytical chemistry, biochemistry, biotechnology, and bioinformatics, and a valuable resource for everyone working in biologically oriented mass spectrometry." (Andreas Tholey, Analytical and Bioanalytical Chemistry, September, 2009)

Read More Show Less

Product Details

  • ISBN-13: 9781934115480
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/12/2008
  • Series: Methods in Molecular Biology Series, #492
  • Edition description: 2nd ed. 2009
  • Edition number: 2
  • Pages: 470
  • Product dimensions: 7.70 (w) x 10.30 (h) x 1.10 (d)

Table of Contents



• Generation and Analysis of Multidimensional Protein Identification Technology Datasets

Selene K. Swanson, Laurence Florens and Michael P. Washburn
• Quantitative Peptide and Protein Profiling by Mass Spectrometry

Alexander Schmidt, Birgit Bisle and Thomas Kislinger
• Label Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach

Kim K. Hixson
• Classical Proteomics: Two-dimensional Electrophoresis/MALDI Mass Spectrometry

Ursula Zimny-Arndt, Monika Schmid, Renate Ackermann and Peter R. Jungblut
• The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression

Rajat Sapra
• Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins

Christian G. Huber, Anna-Maria Timperio, Hansjörg Toll and Lello Zolla
• High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation

Jesper V. Olsen and Boris Macek
• Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra

Amy Nichols and Forest White
• Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry

Yehia Mechref, Milan Madera and Milos V. Novotny
• Structure Analysis of N-glycoproteins

Stefanie Henning, Jasna Peter-Katalinic and Gottfried Pohlentz
• Capillary Zone Electrophoresis-mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins

Christian Neusüß and Matthias Pelzing
• Top-down Proteomics on a High-field Fourier Transform Ion Cyclotron Resonance Mass spectrometer

Séverine A. Ouvry-Patat, Matthew P. Torres, Craig Gelfand, Hung-Hiang Quek, Michael Easterling, J. Paul Speir and Christoph H. Borchers
• Capillary Isoelectric Focusing/Reversed Phase Liquid Chromatography/Mass Spectrometry

Cheng S. Lee and Brian M. Balgley
• Integrating Accelerated Tryptic Digestion into Proteomics Workflows

Gordon W. Slysz and David C. Schriemer
• Hydrogen/Deuterium Exchange Mass Spectrometry

Xuguang Yan and Claudia S. Maier
• Mass Spectrometry Detection and Characterization of Noncovalent Protein Complexes

Sheng Yin and Joseph A. Loo
• Chemical Cross-linking for Protein-protein Interaction Studies

Xiaoting Tang and James E. Bruce
• Tissue Analysis with High Resolution Imaging Mass Spectrometry

A.F. Maarten Altelaar and Ron M.A. Heeren
• Proteomic Global Profiling for Cancer Biomarker Discovery

Samir Hanash
• Analysis of Protein Glycosylation and Phosphorylation Using Liquid Phase Separation, Protein Microarray Technology and Mass Spectrometry

Jia Zhao, Tasneem H. Patwa, Manoj Pal, Weilian Qiu and David M. Lubman
• Transthyretin Mass Determination for Detection of Transthyretin Familial Amyloid

John F. O’Brien and H. Robert Bergen, III
• Characterization of Microorganisms by MALDI Mass Spectrometry

Catherine E. Petersen, Nancy B. Valentine and Karen L. Wahl
• Mass Spectrometric Characterization Of Neuropeptides

Stephanie S. DeKeyser, James A. Dowell and Lingjun Li
• Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods

Scott A. McLuckey
• Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics

Roman A. Zubarev
• Two-Dimensional Ion Mobility Analyses of Proteins and Peptides

Alexandre A. Shvartsburg, Keqi Tang and Richard D. Smith
• Proteomics for Validation of Automated Gene Model Predictions

Kemin Zhou, Ellen A. Panisko, Jon K. Magnuson, Scott E. Baker and Igor V. Grigoriev
• Support Vector Machines for Improved Peptide Identification from Tandem Mass Spectrometry Database Search

Bobbie-Jo M. Webb-Robertson

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)