Medical Mycology: Cellular and Molecular Techniques / Edition 1

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Overview

Medical Mycology: Cellular and Molecular techniques is a clear and concise overview of the subject that details the techniques essential for ongoing research in the area. Drawing together contributions from both scientists and clinicians working in the field, the text will provide a valuable perspective on the applicability of specific techniques to patient care.

A wide range of molecular, immunological and cytological techniques are discussed throughout, with the inclusion of protocol section in each chapter designed to provide both a background a up-to-date account of the applications of each procedure. Every technique is fully referenced and illustrations are provided where required to enhance student understanding.

  • comprehensive introduction to the key techniques critical to the study of medical mycology
  • clear explanation of how each technique is applied in the lab
  • contributions from internationally recognised experts in the field
  • outlines the background to many techniques required for the successful completion of a research project

An invaluable reference for students of microbiology, biochemistry and molecular biology as well as postgraduates and researchers in the field of medical mycology looking for an up-to-date overview of the latest laboratory techniques.

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Editorial Reviews

Doody's Review Service
Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This comprehensive techniques book specifically addresses the techniques used to study fungi. Each chapter gives detailed protocols and advice for experimental work with fungal agents.
Purpose: The goal was to assemble detailed procedures on a variety of experimental techniques currently available for the study of fungal virulence. Use of these techniques will eventually produce an enhanced understanding of fungal pathogenesis and assist in the development of new antifungal agents. These chapters are written by expert scientists working with fungal pathogens and they bring a wealth of experience with each technique presented.
Audience: This book is written for scientists and students working in the area of fungal pathogenicity. The authors are all recognized experts in the area of fungal research.
Features: The 14 chapters provide detailed information on a variety of techniques used to study fungal biology. These techniques range from the visualization of fungal elements in tissue to the evaluation of antifungal activity and to the use of animal models. Several chapters give thorough protocols for the proteomic analysis of fungal cells and share advice regarding various methods used to study protein expression. Several chapters describe techniques used to study the genetic organization and gene expression of fungal cells. These even describe the production of knock-out organisms to study the function of the specific gene.
Assessment: This very useful book expansively covers laboratory techniques used to study fungal agents. There is no other current book that gives the details on experimental procedures that this one does. This is also a very affordable book — for less than $100 a laboratory can acquire a wealth of information and advice on a wide range of procedures. This would be a very worthwhile acquisition for investigators working with fungi.
From the Publisher
"There is no other current book that gives the details on experimental procedures that this one does…a very worthwhile acquisition for investigators working with fungi." (Doody's Health Services)

"Will be very useful to many students and bench scientists wishing to expand their repertoire of practical skills in medical mycology." (Microbiology Today, March 2008)

Medical Mycology is a useful guide for molecular, immunological, and cytological techniques that will provide useful to researchers and students alike. (The Yale Journal of Biology and Medicine, June 2007)

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Product Details

  • ISBN-13: 9780470019238
  • Publisher: Wiley
  • Publication date: 1/2/2007
  • Edition number: 1
  • Pages: 348
  • Sales rank: 1,458,427
  • Product dimensions: 6.83 (w) x 9.92 (h) x 1.06 (d)

Meet the Author

Editor: Dr Kevin Kavanagh, Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland

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Table of Contents

Preface.

List of Contributors.

1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry (Malcolm D. Richardson, Riina Rautemaa and Jarkko Hietanen).

2 Transmission Electron Microscopy of Pathogenic Fungi (Guy Tronchin and Jean-Philippe Bouchara).

3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi (Maria Simitsopoulou and Emmanuel Roilides).

4 Determination of the Virulence Factors of Candida Albicans and Related Yeast Species (Khaled H. Abu-Elteen and Mawieh Hamad).

5 Analysis of Drug Resistance in Pathogenic Fungi (Gary P. Moran, Emmanuelle Pinjon, David C. Coleman and Derek J. Sullivan).

6 Animal Models for Evaluation of Antifungal Efficacy Against Filamentous Fungi (Eric Dannaoui).

7 Proteomic Analysis of Pathogenic Fungi (Alan Murphy).

8 Extraction and Detection of DNA and RNA from Yeast (Patrick Geraghty and Kevin Kavanagh).

9 Microarrays for Studying Pathogenicity in Candida Albicans (André Nantel, Tracey Rigby, Hervé´ Hogues and Malcolm Whiteway).

10 Molecular Techniques for Application with Aspergillus Fumigatus (Nir Osherov and Jacob Romano).

11 Promoter Analysis and Generation of Knock-out Mutants in Aspergillus Fumigatus (Matthias Brock, Alexander Gehrke, Venelina Sugareva and Axel A. Brakhage).

12 Microarray Technology for Studying the Virulence of Aspergillus Fumigatus (Darius Armstrong-James and Thomas Rogers).

13 Techniques and Strategies for Studying Virulence Factors in Cryptococcus Neoformans (Nancy Lee and Guilhem Janbon).

14 Genetic Manipulation of Zygomycetes (Ashraf S. Ibrahim and Christopher D. Skory).

Index.

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