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From The CriticsReviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This comprehensive techniques book specifically addresses the techniques used to study fungi. Each chapter gives detailed protocols and advice for experimental work with fungal agents.
Purpose: The goal was to assemble detailed procedures on a variety of experimental techniques currently available for the study of fungal virulence. Use of these techniques will eventually produce an enhanced understanding of fungal pathogenesis and assist in the development of new antifungal agents. These chapters are written by expert scientists working with fungal pathogens and they bring a wealth of experience with each technique presented.
Audience: This book is written for scientists and students working in the area of fungal pathogenicity. The authors are all recognized experts in the area of fungal research.
Features: The 14 chapters provide detailed information on a variety of techniques used to study fungal biology. These techniques range from the visualization of fungal elements in tissue to the evaluation of antifungal activity and to the use of animal models. Several chapters give thorough protocols for the proteomic analysis of fungal cells and share advice regarding various methods used to study protein expression. Several chapters describe techniques used to study the genetic organization and gene expression of fungal cells. These even describe the production of knock-out organisms to study the function of the specific gene.
Assessment: This very useful book expansively covers laboratory techniques used to study fungal agents. There is no other current book that gives the details on experimental procedures that this one does. This is also a very affordable book — for less than $100 a laboratory can acquire a wealth of information and advice on a wide range of procedures. This would be a very worthwhile acquisition for investigators working with fungi.