Membrane Biogenesis: Methods and Protocols

Overview

Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the ...

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Overview

Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.

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Product Details

  • ISBN-13: 9781627034869
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/11/2013
  • Series: Methods in Molecular Biology , #1033
  • Edition description: 2013
  • Edition number: 1
  • Pages: 404
  • Product dimensions: 7.20 (w) x 10.10 (h) x 1.10 (d)

Table of Contents

Part I: Investigation of Membrane Lipids

1. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry

Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger

2. Thin-Layer Chromatography of Phospholipids

Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg

3. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics

Martina Gsell and Günther Daum

Part II: Analysis of Structure and Topology of Membrane Proteins

4. Using 2D Crystals to Analyze the Structure of Membrane Proteins

Ian Collinson, Janet Vonck, and Dilem Hizlan

5. Crystallization of Membrane Proteins

Florian G. Müller and C. Roy D. Lancaster

6. Molecular Dynamics Simulations of Membrane Proteins

Kristyna Pluhackova, Tsjerk Wassenaar, and Rainer A. Böckmann

7. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins

Christine T. Schwall and Nathan N. Alder

8. Topology Determination of Untagged Membrane Proteins

Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner

9. selfassembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses

Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, and Maik S. Sommer

Part III: Studying Protein-Protein and Protein-Lipids Interactions within Membranes

10. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane

Milit Marom and Abdussalam Azem

11. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance

Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, and Arnold J.M Driessen

12. Peptide Interaction with and Insertion into Membranes

Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai

13. Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems

Joseph D. Unsay and Ana J. García-Sáez

14. Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast

Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo

15. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins

Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, and Christine Ebel

16. Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry

Carolyn Vargas, Johannes Klingler, and Sandro Keller

Part IV: Membrane Biogenesis and Dynamics

17. Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria

Dirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, and Benedikt Westermann

18. Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells

Johanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, and Richard Zimmermann

19. An Assay to Monitor the Membrane Integration of Single-Span Proteins

Katrin Krumpe and Doron Rapaport

20. Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria

Daniel Weckbecker and Johannes M. Herrmann

Part V: Functional Analysis of Membrane Proteins

21. Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes

Martin van der Laan, Ralf M. Zerbes, and Chris van der Does

22. Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes

Philipp Bartsch, Anke Harsman, and Richard Wagner

23. Quantification of Protein Complexes by Blue Native Electrophoresis

Juliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, and Ilka Wittig

24. Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions

Anna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew, and Jan-Willem de Gier

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