Meningococcal Vaccines: Methods and Protocols / Edition 1

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Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. Timely and comprehensive, Meningococcal Vaccines: Methods and Prools provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus, and to develop, evaluate, and implement successful new meningococcal vaccines.

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Editorial Reviews

From the Publisher
"This volume is an impressive collection of the latest techniques in the development, evaluation and application of meningococcal vaccines. The easy-to-follow prools each preceded by a brief introduction into the topic were provided by leading scientists in the field. Andrew Pollard and Martin Maiden did a great job in assembling these prools into one volume - a work we all in the meningococcal field have waited for." - International Journal of Medical Microbiology.

"The strength of this text is that it provides a key reference source, enabling researchers in the field to avoid reinventing the wheel. The text also provides the basis for the development of new ideas. Each chapter takes a step-by-step approach with clearly set out notes highlighting the problem areas. All the chapters have comprehensive bibliographies, and are well structured. The text is easily accessible and is recommended to those who are seeking to critically appraise the field of meningococcal vaccines." - Epidemiology and Infection

"This book and its accompanying volume are well produced, easy to read and well edited. Andrew Pollard and Martin Maiden are to be congratulated in bringing together in these two volumes an enormous amount of information about this very important topical infection." -Molecular Biotechnology

Reports on the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines against Neisseria meningitidis, the causative agent of meningococcal disease. Scientists, clinicians, and public health physicians describe approaches to vaccine design and detail the assessment of immune response to vaccine candidates and novel vaccine formulations. A test case is presented on the implementation of a new vaccine in the UK. Some specific topics include recombinant proteins in vaccine development, experiences with nasal vaccines, and T-cell responses against meningococcal antigens. Pollard is affiliated with the BC Research Institute for Children's and Women's Health, Canada. Maiden is affiliated with the Wellcome Trust Center for the Epidemiology of Infectious Disease, UK. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9780896038011
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 6/28/2001
  • Series: Methods in Molecular Medicine Series , #66
  • Edition description: 2001
  • Edition number: 1
  • Pages: 421
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.00 (d)

Table of Contents

Meningococcal Vaccines and Vaccine Developments
Ian M. Feavers

Immune Response and Host-Pathogen Interactions
Andrew J. Pollard and David Goldblatt

Purification of Capsular Polysaccharide
Qingling Yang and Harold Jennings

Protein-Polysaccharide Conjugation
Zhongwu Guo and Harold Jennings

Zhongwu Guo and Harold Jennings

Outer Membrane Protein Purification,
armen Arigita, Wim Jiskoot, Matthijs R. Graaf, and Gideon F. A. Kersten

Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease
Carl E. Frasch, Loek van Alphen, Johan Holst, Jan T. Poolman, and Einar Rosenqvist

Methods for Manipulation of Transferrin-Binding Proteins
Leanne M. DeWinter and Anthony B. Schryvers

Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins
Richard F. Collins and Jeremy P. Derrick

Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics
Janet Suker and Bambos M. Charalambous

Construction of porA Mutants
Peter van der Ley and Loek van Alphen

Construction of LPS Mutants
Peter van der Ley and Liana Steeghs

Recombinant Proteins in Vaccine Development
Myron Christodoulides, Keith A. Jolley, and John E. Heckels

Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries
Paul J. Brett, Ian M. Feavers, and Bambos M. Charalambous

Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity
Alexei A. Delvig, John H. Robinson, and Lee Wetzler

Early Experiences with Nasal Vaccines against Meningococcal Disease
Johan Holst and Bjørn Haneberg

Animal Models for Meningococcal Disease
Andrew R. Gorringe, Karen M.Reddin, Pierre Voet, and Jan T. Poolman

Determination of Antibody Responses to Meningococcal Antigens by ELISA
Einar Rosenqvist, Helena Käyhty, and Andrew J. Pollard

Immunoblot Analysis of Sera from Patients and Vaccinees
Elisabeth Wedege

Serogroup B and C Serum Bactericidal Assays
Ray Borrow and George M. Carlone

A Modified ELISA for Measurement of High-Avidity IgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers
Dan M. Granoff and John J. Donnelly

Whole-Blood Model
Catherine A. Ison

Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry
Terje E. Michaelsen and Audun Aase

T-Cell Responses Against Meningococcal Antigens
Lisbeth Meyer Næss, Fredrik Oftung, Audun Aase, Terje E. Michaelsen and Andrew J. Pollard

CD4 T-Cell Epitope Mapping
Alexei A. Delvig and John H. Robinson

Epitope Mapping
John E. Heckels and Myron Christodoulides

Meningococcal Vaccine Trials
Paddy Farrington and Elizabeth Miller

The Introduction of Group C Conjugate Meningococcal Vaccine into the UK
David M. Salisbury

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