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Meningococcal Vaccines: Methods and Protocols / Edition 1
     

Meningococcal Vaccines: Methods and Protocols / Edition 1

by Andrew J. Pollard
 

ISBN-10: 0896038017

ISBN-13: 9780896038011

Pub. Date: 06/28/2001

Publisher: Springer-Verlag New York, LLC

Andrew Pollard and Martin Maiden have assembled an impressive collecti on of the latest molecular and cellular techniques for the development , evaluation, and implementation of vaccines to be used against this d readed disease. The contributorsyleading scientists, clinicians, and p ublic health physiciansydescribe in detail the many approaches to vacc ine design,

Overview

Andrew Pollard and Martin Maiden have assembled an impressive collecti on of the latest molecular and cellular techniques for the development , evaluation, and implementation of vaccines to be used against this d readed disease. The contributorsyleading scientists, clinicians, and p ublic health physiciansydescribe in detail the many approaches to vacc ine design, as well as the assessment of immune response to vaccine ca ndidates and novel vaccine formulations. Timely and comprehensive, Men ingococcal Vaccines: Methods and Protocols provides the scientist, pub lic health physician, epidemiologist, clinical microbiologist, and cli nician with the essential tools to lay bare the secrets of the meningo coccus, and to develop, evaluate, and implement successful new meningo coccal vaccines.

Product Details

ISBN-13:
9780896038011
Publisher:
Springer-Verlag New York, LLC
Publication date:
06/28/2001
Series:
Methods in Molecular Medicine Series , #66
Edition description:
2001
Pages:
421
Product dimensions:
6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Meningococcal Vaccines and Vaccine Developments
Ian M. Feavers

Immune Response and Host-Pathogen Interactions
Andrew J. Pollard and David Goldblatt

Purification of Capsular Polysaccharide
Qingling Yang and Harold Jennings

Protein-Polysaccharide Conjugation
Zhongwu Guo and Harold Jennings

N-Propionylation
Zhongwu Guo and Harold Jennings

Outer Membrane Protein Purification,
armen Arigita, Wim Jiskoot, Matthijs R. Graaf, and Gideon F. A. Kersten

Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease
Carl E. Frasch, Loek van Alphen, Johan Holst, Jan T. Poolman, and Einar Rosenqvist

Methods for Manipulation of Transferrin-Binding Proteins
Leanne M. DeWinter and Anthony B. Schryvers

Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins
Richard F. Collins and Jeremy P. Derrick

Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics
Janet Suker and Bambos M. Charalambous

Construction of porA Mutants
Peter van der Ley and Loek van Alphen

Construction of LPS Mutants
Peter van der Ley and Liana Steeghs

Recombinant Proteins in Vaccine Development
Myron Christodoulides, Keith A. Jolley, and John E. Heckels

Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries
Paul J. Brett, Ian M. Feavers, and Bambos M. Charalambous

Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity
Alexei A. Delvig, John H. Robinson, and Lee Wetzler

Early Experiences with Nasal Vaccines against Meningococcal Disease
Johan Holst and Bjørn Haneberg

Animal Models for Meningococcal Disease
Andrew R. Gorringe, Karen M.Reddin, Pierre Voet, and Jan T. Poolman

Determination of Antibody Responses to Meningococcal Antigens by ELISA
Einar Rosenqvist, Helena Käyhty, and Andrew J. Pollard

Immunoblot Analysis of Sera from Patients and Vaccinees
Elisabeth Wedege

Serogroup B and C Serum Bactericidal Assays
Ray Borrow and George M. Carlone

A Modified ELISA for Measurement of High-Avidity IgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers
Dan M. Granoff and John J. Donnelly

Whole-Blood Model
Catherine A. Ison

Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry
Terje E. Michaelsen and Audun Aase

T-Cell Responses Against Meningococcal Antigens
Lisbeth Meyer Næss, Fredrik Oftung, Audun Aase, Terje E. Michaelsen and Andrew J. Pollard

CD4 T-Cell Epitope Mapping
Alexei A. Delvig and John H. Robinson

Epitope Mapping
John E. Heckels and Myron Christodoulides

Meningococcal Vaccine Trials
Paddy Farrington and Elizabeth Miller

The Introduction of Group C Conjugate Meningococcal Vaccine into the UK
David M. Salisbury

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