Metabolomics Tools for Natural Product Discovery: Methods and Protocols

Overview

Classical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation. Therefore, it is worthwhile to summarize recent developments with current analytical platforms and highlight how metabolomics is being integrated into this classical field to dereplicate and profile natural product extracts. Metabolomics Tools for Natural Product Discoveries: Methods and Prools aims to unite diverse and recently ...

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Overview

Classical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation. Therefore, it is worthwhile to summarize recent developments with current analytical platforms and highlight how metabolomics is being integrated into this classical field to dereplicate and profile natural product extracts. Metabolomics Tools for Natural Product Discoveries: Methods and Prools aims to unite diverse and recently developed methodologies and prools in order to identify bioactive secondary metabolites for the purpose of drug discovery. Some topics covered in this volume include applications for the extraction of selected natural products from less common sources such as bryophytes and hard corals, various biological assays, comprehensive applications and strategies for GC-MS, LC-MS, and NMR, as well as prools and strategies for the structure elucidation of isolated natural products. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible Metabolomics Tools for Natural Product Discoveries: Methods and Prools seeks to serve both professionals and research students with its well-honed methodologies for natural product isolation, biomarker discovery, dereplication, biological assays, and comprehensive metabolomic platforms available for high-throughput analyses.

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Product Details

  • ISBN-13: 9781627035767
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/6/2013
  • Series: Methods in Molecular Biology Series , #1055
  • Edition description: 2013
  • Edition number: 1
  • Pages: 311
  • Product dimensions: 6.90 (w) x 10.00 (h) x 0.90 (d)

Table of Contents

1. Bryophytes: Liverworts, Mosses and Hornworts – Extraction and Isolation Procedures

Yoshinori Asakawa and Agnieszka Ludwiczuk

2. Plant Tissue Extraction for Metabolomics

Ute Roessner and Daniel Anthony Dias

3. Detection of Polar Metabolites through the use of Gas Chromatography – Mass Spectrometry

David P. De Souza

4. A Robust GC-MS Method for the Quantitation of Fatty Acids in Biological Systems

Nirupama Samanmalie Jayasinghe and Daniel Anthony Dias

5. A Workflow from Untargeted LC-MS Profiling to Targeted Natural Product Isolation

Damien L. Callahan and Candace E. Elliot

6. Lipidomics –Extraction Prools for Biological Matrices

Thusitha Wasntha Thilanka Rupasinghe

7. Metabolite Analysis of Biological Fluids and Tissues by Proton Nuclear Magnetic Resonance Spectroscopy

John Robert Sheedy

8. NMR Spectroscopy: Structure Elucidation of Cycloelatenene A: A Natural Product Case Study

Sylvia Urban and Daniel Anthony Dias

9. NMR-Based Metabolomics: A Probe to Utilize Biodiversity

Lúcia P. Santos Pimenta, Hye Kyong Kim, Robert Verpoorte, and Young Hae Choi

10. Extraction Prool for Non-Targeted NMR and LC-MS Metabolomics-Based Analysis of Hard Coral and their Algal Symbionts

Benjamin R. Gordon, William Leggat, and Cherie Motti

11. Determination of Absolute Configuration Using Single Crystal X-ray Diffraction

Abigail L. Albright and Jonathan M. White

12. Natural Product Chemistry in Action: The Synthesis of Melatonin Metabolites K1 and K2

Helmut M. Hügel and Oliver A.H. Jones

13. Discovery, Biosynthesis, and Rational Engineering of Novel Enterocin and Wailupemycin Polyketide Analogues

John A. Kalaitziz

14. Bioassays for Anticancer Activities

Janice McCauley, Ana Zivanovic, and D. Skropeta

15. Screening for Anti-Diabetic Activities

Rima Caccetta and Hani Al Salami

16. Screening for Antibacterial, Antifungal and Anti-Quorum Sensing Activity

Elisa J. Hayhoe and Enzo A. Palombo

17. Metabolomics and Dereplication Strategies in Natural Products

Ahmed Fares Tawfike, Christina Viegelmann, and RuAngelie Edrada-Ebel

18. Bridging the Gap: Basic Metabolomics Methods for Natural Product Chemistry

Oliver A.H. Jones and Helmut M. Hügel

19. Strategies in Biomarker Discovery. Peak Annotation by MS and Targeted LC-MS Micro-Fractionation for de novo Structure Identification by Micro-NMR

Philippe J. Eugster, Gaëtan Glauser, and Jean-Luc Wolfender

20. Statistical Analysis of Metabolomics Data

Alysha M. De Livera, Moshe Olshansky, and Terence P. Speed

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