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Methods for General and Molecular Microbiology / Edition 3

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A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods.

  • Provides a comprehensive compendium of methods used in general and molecular microbiology.
  • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis.
  • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions.
  • Presents methods for bacteria, archaea, and for the first time a section on mycology.
  • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

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Editorial Reviews

Doody's Review Service
Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This new edition of a classic reference in microbiology is put together well. In over 1,000 pages, this book covers the multiple topics and techniques used to investigate microorganisms. With the previous edition of this book over 10 years old, this update was necessary to include the many new investigational tools developed since then.
Purpose: The purpose of this book has remained constant over the years. The previous versions have become the standard source of information for microbiologists in all fields. This book is the "first source for traditional methods of microbiology as well as modern molecular biology." The new version includes new methods and theories as well as new areas of microbiology.
Audience: All scientists and students of microbiology will find this book a valuable resource. It is written at a level appropriate for professional scientists as well as training scientists. All of the chapters are well organized and are written by well established scientists familiar with both the science and technical details.
Features: This book covers both the theory and practical considerations of the various techniques used to study microorganisms. The different sections cover microscopy, analysis of enzymatic reactions, microbial metabolism, and molecular analysis. The authors emphasize the need for multiple types of analyses in order to answer the multiple questions in microbiology. Each chapter contains the basic background and historical information needed to understand the new methods and each method is presented in detail that makes it easy to follow. There is also discussion of potential problems and how to avoid them. In some cases the authors have listed the equipment and reagents needed to successfully use the technique.
Assessment: It is hard to improve on success, but this latest edition of a treasured classic has exceeded expectations. The original edition was my first "real" microbiology textbook and it will be good to have an up-to-date version. This latest edition can be relied upon to teach the new generation of scientists and will remain a treasure in my collection of classic references for many years.
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Product Details

  • ISBN-13: 9781555812232
  • Publisher: ASM Press
  • Publication date: 9/28/2007
  • Edition description: REV
  • Edition number: 3
  • Pages: 1090
  • Product dimensions: 8.30 (w) x 11.00 (h) x 2.20 (d)

Table of Contents

Table of Contents

I. Morphology and Ultrastructure

Introduction to Morphology and Ultrastructure, T. J. Beveridge, Editor

1. Light Microscopy, R. G. E. Murray and Carl F. Robinow
2. Sampling and Staining for Light Microscopy, Terry J. Beveridge, J. R. Lawrence, and Robert G. E. Murray
3. Laser Scanning Microscopy, John R. Lawrence and T. R. Neu
4. Electron Microscopy, Terry J. Beveridge, Dianne Moyles, and Bob Harris
5. Computational Image Analysis and Reconstruction from Transmission Electron Micrographs, George Harauz
6. Atomic Force Microscopy, Yves F. Dufrêne
7. Cell Fractionation, Susan F. Koval and G. Dennis Sprott
8. Antigen-Antibody Reactions, Lucy M. Mutharia and Joseph S. Lam

II. Growth

Introduction to Growth, John A. Breznak, Editor

9. Growth Measurement, Arthur L. Koch
10. Nutrition and Media, David Emerson, Jane Tang, Rosali J. Cote, and Robert L. Gherna
11. Enrichment and Isolation, Andreas Teske, Herbert Cypionka, John G. Holt, and Noel R. Krieg
12. Culture Techniques, Syed A. Hashsham
13. Energetics, Stoichiometry, and Kinetics of Microbial Growth, Syed A. Hashsham and Sam W. Bauschke
14. Physicochemical Factors in Growth, John A. Breznak and Ralph N. Costilow
15. Phenotypic Characterization and the Principles of Comparative Systematics, Brian J. Tindall, Johannes Sikorski, Robert A. Smibert, and Noel R. Krieg
16. General Methods to Investigate Microbial Symbioses, Todd A. Ciche and Shana K. Goffredi

III. Metabolism

Introduction to Metabolism, C. A. Reddy, Editor

17. Physical Analysis and Purification Methods, Scott B. Mulrooney, Willis A. Wood, and J. Robert Paterek
18. Chemical Analysis, Lacy Daniels, Richard S. Hanson, and Jane A. Phillips
19. Enzymatic Activity, Robert P. Hausinger and Allen T. Phillips
20. Permeability and Transport, Robert E. Marquis
21. Bacterial Respiration, Robert P. Gunsalus, Gary Cecchini, and Imke Schröder
22. Carbohydrate Fermentations, R. Meganathan, Yamini Ranganathan, and C. A. Reddy
23. Metabolism of Aromatic Compounds, Jerome J. Kukor, Boris Wawrik, and Gerben J. Zylstra
24. Cellulases, Hemicellulases, and Pectinases, Michael E. Himmel, John O. Baker, William S. Adney, and Stephen R. Decker
25. Lignin and Lignin-Modifying Enzymes, Carlos G. Dosoretz and C. A. Reddy

IV. Molecular Genetics

Introduction to Molecular Genetics, C. A. Reddy, Editor
L. R. Snyder, Co-Editor
26. Similarity Analysis of DNAs, John L. Johnson and William B. Whitman
27. Nucleic Acid Analysis, William Hendrickson and Donald Walthers
28. Measuring Spontaneous Mutation, Patricia L. Foster
29. Transposon Mutagenesis, Silvia Rossbach and Frans J. de Bruijn
30. Plasmids, Marcelo E. Tolmasky, Luis A. Actis, Timothy J. Welch, and Jorge H. Crosa
31. Gene Transfer in Gram-Negative Bacteria, Joseph E. Peters
32. Gene Exchange in Gram-Positive Bacteria, Christopher J. Kristich, Christine E. Salomon, and Gary M. Dunny
33. Genetics of Archaea, Kevin R. Sowers, Paul H. Blum, and Shiladitya DasSarma
34. Genetic Manipulations Using Phages, Graham F. Hatfull, Debbie Jacobs-Sera, Michelle H. Larsen, and William R. Jacobs, Jr.

V. Community and Genomic Analysis

Introduction to Community and Genomic Analysis, Thomas M. Schmidt, Editor

35. Characterization of Bacterial Genome Sequences by Similarity Searching, William R. Pearson
36. Reconstructing and Interpreting Evolutionary Relationships, Christophe J. Douady and Camilla L. Nesbø
37. Microbial Nucleotide Fingerprints in Nature, David M. Karl
38. Construction of BAC and Fosmid Libraries from Naturally Occurring Microbial Populations, Edward F. DeLong
39. Single Cell Identification by Fluorescence In Situ Hybridization, Bernhard M. Fuchs, Jakob Pernthaler, and Rudolf Amann
40. Measurement of rRNA Abundance by Hybridization with Oligodeoxynucleotide Probes, Daniel H. Buckley and Thomas M. Schmidt
41. Analysis of Microbial Communities with Denaturing Gradient Gel Electrophoresis and Terminal Restriction Fragment Length Polymorphism, Terence L. Marsh and Cindy H. Nakatsu

VI. Mycology

Introduction to Mycology, George A. Marzluf, Editor

42. Methods for Studying Terrestrial Fungal Ecology and Diversity, R. G. Thorn, J. Scott, and M. A. LaChance
43. Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi, George A. Marzluf
44. Microbiological and Genetic Methods for Filamentous Fungi, Rowland H. Davis and A. John Clutterbuck
45. Principles and Practice of DNA Microarray Technology, Krishnamurthy Natarajan, Matthew J. Marton, and Alan G. Hinnebusch

46. Laboratory Safety, W. Emmett Barkley and C. A. Mickelson
47. Culture Preservation, Robert L. Gherna and C. A. Reddy

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