Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci / Edition 1

by Paula M. Fives-Taylor, Donald J. LeBlanc

Overview

This book is devoted entirely to methods developed in and for studies of members of the bacterial family Strepoccaceae. Many of the studies that have been conducted on the Strepoccaceae were initiated because of the diseases they cause, or to enhance their utility from an industrial perspective. However, the results of many of these investigations have demonstrated a complexity among some members of the family that warrants an interest in them in their own right, apart from or in addition to any biomedical or industrial considerations.
It is therefore hoped and expected that the advanced methods contained in this book will be of interest to those who work with the strepocci and other Gram-positive organisms, to researchers interested in industrial and medical microbiology and to any researcher who seeks to obtain a better understanding of how microorganisms interact with each other, their environment and their hosts.

Product Details

ISBN-13:	9780792358152
Publisher:	Springer Netherlands
Publication date:	07/31/1999
Edition description:	1998
Pages:	256
Product dimensions:	8.27(w) x 11.69(h) x 0.39(d)

About the Author

Fives-Taylor, Paula M. (Univ of Vermont); LeBlanc, Donald J. (Lilly Research Laboratories)

Table of Contents

Tn917 transponson mutagenesis and marker rescue of interrupted genes of Strepoccus mutans.- Site-specific homologous recombination mutagenesis in group B strepocci.- Targeted mutagenesis of enterococcal genes.- A lacoccal pWV01-based integration toolbox for bacteria.- Vectors containing strepoccal bacteriophage integrases for site-specific gene insertion.- Strepoccal integration vectors for gene inactivation and cloning.- Induction of transformation in strepocci by synthetic competence stimulating peptides.- Characterization of the lacoccal conjugative element pRS01 using IS946-mediated mutagenesis.- Use of electroportation in genetic analysis of enterococcal virulence.- Genetic transfer methods for Strepoccus sobrinus and other oral strepocci.- Isolation of enterococcal antigen-encoding genes from genomic libraries.- A simple microtiter plate screening assay for bacterial invasion or adherence.- End-probing: A non-radioactive approach to mapping transponson insertions.- A method for mapping phage-inducible promoters for use in bacteriophage-triggered defense systems.- Secretion of heterologous proteins by genetically engineered Strepoccus gordonii.- Examination of strepoccal gene expression in the mammalian environment.- Analysis of adherence-associated gene expression in Strepoccus parasangusis: A method for RNA isolation.- Development of an integrative, lacZ transcriptional-fusion plasmid vector for Strepoccus mutans and its use to isolate expressed genes.- Use of proteomics and PCR to elucidate changes in protein expression in oral strepocci.- The use of continuous flow bioreactors to explore gene expression and physiology of suspended and adherent populations of oral strepocci.- In vitro systems for investigating group Bstrepoccal: host cell and extracellular matrix interactions.- The rat model of endocarditis.- Lipoproteins and other cell-surface associated proteins in strepocci.- Growth of Strepoccal mutons in an iron-limiting medium.- Identification of oral strepocci using PCR-based, reverse-capture, checkerboard hybridization.- Pulsed-field gel electrophoresis as an epidemiologic tool for enterococci and strepocci.- Cell-based panning as a means to isolate phage display Fabs specific for a bacterial surface protein.