Methods in Biological Oxidative Stress / Edition 1

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More About This Textbook


Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method.

Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry.

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Product Details

  • ISBN-13: 9781617372001
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/9/2010
  • Series: Methods in Pharmacology and Toxicology Series
  • Edition description: Softcover reprint of hardcover 1st ed. 2003
  • Edition number: 1
  • Pages: 216
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.53 (d)

Table of Contents

Part I. Lipids

Measurement of Fat-Soluble Vitamins and Antioxidants by HPLC With Electrochemical Array Detection
Paul H. Gamache, Paul A. Ullucci, Joe A. Archangelo, and Ian N. Acworth

Analysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC With Fluorescence Detection
Mark A. Lovell and William R. Markesbery

Measurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Joshua P. Fessel and L. Jackson Roberts, II

Analysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
L. Jackson Roberts, II and Jason D. Morrow

Measurement of F4-Neuroprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Nathalie Bernoud-Hubac and L. Jackson Roberts, II

Immunoassays for Lipid Peroxidation End Products: One-Hour ELISA for Protein-Bound Acrolein and HNE
Kimihiko Satoh and Koji Uchida

Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biological Matrices
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd

HPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitration Products in Human Plasma
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd

Part II. DNA, Protein, and Amino Acids

Electron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Membrane Protein Oxidation
D. Allan Butterfield

Gas Chromatography-Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids
Joseph P. Gaut, Jaeman Byun, and Jay W. Heinecke

Isotope Dilution Gas Chromatography-Mass Spectrometric Analysis of Tyrosine Oxidation Products in Proteins and Tissues
Jay W. Heinecke

Single-Cell Gel Electrophoresis or Comet Assayof Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis
Mark M. Huycke

Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe
Jun Nakamura and James A. Swenberg

Analysis of Neuroketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry
Nathalie Bernoud-Hubac, Sean S. Davies, Olivier Boutaud, and L. Jackson Roberts, II

Measurement of Isoketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry
Sean S. Davies, Cynthia J. Brame, Olivier Boutaud, Nathalie Bernoud-Hubac, and L. Jackson Roberts, II

Bioassay of 29-Deoxyguanosine/8-Hydroxy-29- Deoxyguanosine by HPLC With Electrochemical/Photodiode Array Detection
Kelly S. Williamson, Kenneth Hensley, Quentin N. Pye, Scott Ferrell, and Robert A. Floyd

HPLC With Electrochemical Detection Analysis of 3-Nitrotyrosine in Human Plasma
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd

Part III. Reactive Oxygen Species and Reactive Nitrogen Species

Protein Carbonyl Levels-An Assessment of Protein Oxidation
Alessandra Castegna, Jennifer Drake, Chava Pocernich, and D. Allan Butterfield

Fluorogenic Analysis of H2O2 in Biological Materials
Kenneth Hensley, Kelly S. Williamson, and Robert A. Floyd

Detection of Reactive Oxygen Species by Flow Cytometry
Alexander Christov, Ladan Hamdheydari, and Paula Grammas

Nitrite Determination by Colorimetric and Fluorometric Greiss Diazotization Assays: Simple, Reliable, High-Throughput Indices of Reactive Nitrogen Species in Cell Culture Systems
Kenneth Hensley, Shenyun Mou, and Quentin N. Pye

Protein Carbonyl Determination Using Biotin Hydrazide
Kenneth Hensley and Kelly S. Williamson

Real-Time, In Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic Analysis of Biliary Flow

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