Methods in Biological Oxidative Stress

Overview

A collection of readily reproducible experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established routine protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, to the electrochemical detection of DNA oxidation products. There are also mass spectrometry...

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Hardcover (2003)
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Overview

A collection of readily reproducible experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established routine protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods measuring lipid oxidation products.

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Product Details

  • ISBN-13: 9780896038158
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 2/15/2010
  • Series: Methods in Pharmacology and Toxicology Series
  • Edition description: 2003
  • Edition number: 1
  • Pages: 216
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.56 (d)

Table of Contents

Foreword v
Preface vii
Contributors xiii
Part I Lipids
1 Measurement of Fat-Soluble Vitamins and Antioxidants by HPLC With Electrochemical Array Detection 3
2 Analysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC With Fluorescence Detection 17
3 Measurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization 23
4 Analysis of F[subscript 2]-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization 33
5 Measurement of F[subscript 4]-Neuroprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization 41
6 Immunoassays for Lipid Peroxidation End Products: One-Hour ELISA for Protein-Bound Acrolein and HNE 49
7 Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biological Matrices 57
8 HPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitration Products in Human Plasma 67
Part II DNA, Protein, and Amino Acids
9 Electron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Mebrane Protein Oxidation 79
10 Gas Chromatography-Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids 87
11 Isotope Dilution Gas Chromatography-Mass Spectrometric Analysis of Tyrosine Oxidation Products in Proteins and Tissues 93
12 Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis 101
13 Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe 109
14 Analysis of Neuroketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry 117
15 Measurement of Isoketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry 127
16 Bioassay of 2'-Deoxyguanosine/8-Hydroxy-2'-Deoxyguanosine by HPLC With Electrochemical/Photodiode Array Detection 137
17 HPLC With Electrochemical Detection Analysis of 3-Nitrotyrosine in Human Plasma 151
Part III Reactive Oxygen Species and Reactive Nitrogen Species
18 Protein Carbonyl Levels--An Assessment of Protein Oxidation 161
19 Fluorogenic Analysis of H[subscript 2]O[subscript 2] in Biological Materials 169
20 Detection of Reactive Oxygen Species by Flow Cytometry 175
21 Nitrite Determination by Colorimetric and Fluorometric Greiss Diazotization Assays: Simple, Reliable, High-Throughput Indices of Reactive Nitrogen Species in Cell-Culture Systems 185
22 Protein Carbonyl Determination Using Biotin Hydrazide 195
23 Real-Time, In-Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic Analysis of Biliary Flow 201
Index 207
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