Methods in Cell Biology: Drosophila Melanogaster: Practical Uses in Cell and Molecular Biology

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More About This Book

Product Details

  • ISBN-13: 9780125641456
  • Publisher: Elsevier Science & Technology Books
  • Publication date: 12/6/1994
  • Series: Methods in Cell Biology, #44
  • Pages: 755

Table of Contents

Preface: Why the Fly?
1 Sources of Information about the Fly: Where to Look It Up
2 Care and Feeding of Drosophila melanogaster
3 Harnessing the Power of Drosophila Genetics
4 From Clone to Mutant Gene
5 Raising Large Quantities of Drosophila for Biochemical Experiments
6 Isolation and Organ Culture of Imaginal Tissues
7 Mass Isolation of Fly Tissues
8 Preparation and Analysis of Pure Cell Populations from Drosophila
9 Transformation Techniques for Drosophila Cell Lines
10 Preparation of Drosophila Nuclei
11 Isolation and Characterization of RNA-Binding Proteins from Drosophila melanogaster
12 Chromatin Assembly Extracts from Drosophila Embryos
13 The Soluble Nuclear Fraction, a Highly Efficient Transcription Extract from Drosophila Embryos
14 Basic Methods for Drosophila Muscle Biology
15 Isolation of Cytoskeletal Proteins from Drosophila
16 Isolation and Analysis of Microtubule Motor Proteins
17 Preparation and Analysis of Membranes and Membrane Proteins from Drosophila
18 Preparation of Extracellular Matrix
19 Looking at Polytene Chromosomes
20 Immunological Methods for Mapping Protein Distributions on Polytene Chromosomes
21 Looking at Drosophila Mitotic Chromosomes
22 Looking at Diploid Interphase Chromosomes
23 Electron Microscopy and EM Immunocytochemistry
24 Imaging Neuronal Subsets and Other Cell Types in Whole-Mount Drosophila Embryos and Larvae Using Antibody Probes
25 Immunofluorescence Analysis of the Cytoskeleton during Oogenesis and Early Embryogenesis
26 High-Resolution Microscopic Methods for the Analysis of Cellular Movements in Drosophila Embryos
27 The Use of Photoactivatable Reagents for the Study of Cell Lineage in Drosophila Embryogenesis
28 Looking at Oogenesis
29 Methods for Quantitative Analysis of Transcription in Larvae and Prepupae
30 In Situ Hybridization to RNA
31 Looking at mRNA Splicing and Transport in Situ
32 EM Methods for Visualization of Genetic Activity from Disrupted Nuclei
33 Ectopic Expression in Drosophila
34 Mosaic Analysis Using FLP Recombinase
35 Analysis of Cellular Adhesion in Cultured Cells
36 Using Inhibitors to Study Embryogenesis
37 Chromophore-Assisted Laser Inactivation of Cellular Proteins
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