Methods in Neuroendocrinology

by Louis D. Van de Kar

Overview

This book describes some of the many different disciplines that are involved in investigations of neuroendocrine control mechanisms. It is intended for students, pre- and post-doctoral fellows, medical residents and others who are interested in neuroendocrine research.

Product Details

ISBN-13:	9781000674347
Publisher:	CRC Press
Publication date:	04/28/2020
Sold by:	Barnes & Noble
Format:	eBook
Pages:	252
File size:	3 MB

About the Author

Van de Kar, Louis D.

Table of Contents

Chapter 1 Functional Morphology of the Immortalized Hypothalamic LHRH Neurons/William C. Wetsel, Istvdn Merchenthaler, and Zsolt Liposits -- Chapter 2 Assessment of Neuropeptide Receptor Gene Expression Using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)/Janice H. Urban -- Chapter 3 Identification of Biotinylated Ligands on Specific Target Cells in the Pituitary Studies of Regulation of Binding/Gwendolyn V Childs -- Chapter 4 Steroid Hormone Receptors and the Assessment of Feedback Sensitivity/Robert J. Handaf Richard H. Price Jr.t and Melinda E. Wilson -- Chapter 5 In Vitro Approaches to Neuroendocrinology/Celia D. Sladek -- Chapter 6 Intracellular Recording from Hypothalamic Cells that Regulate Neuroendocrine Function/Bret N. Smith and F. Edward Dudek -- Chapter 7 Application of In Vivo Electrophysiological Approaches to the Study of Neuroendocrine Systems/Joan M. Lakoski and Jane E. Smith -- Chapter 8 Antisense Oligonucleotides as Tools in Neuroendocrinology/Mariana Morris and PingLi -- Chapter 9 The Role of Biogenic Amines in Neuroendocrine Regulation in ConsciousRats/Gydrgy Bagdy -- Chapter 10 Neuroendocrinology of Stress: Behavioral and Neurobiological Considerations/Bela G.J. Bohus -- Chapter 11 Issues in Quantifying Pulsatile Neurohormone Release -- Johannes D. Veldhuis -- Chapter 12 Neuroendocrine Challenge Tests: What Can We Learn From Them ?/Philip J. Cowen -- Index.

Editorial Reviews

This is the third book in a series entitled Cellular and Molecular Neuropharmacology . Each chapter in this volume focuses on a technique used in neuroendocrine research, an area that bridges neuroscience and endocrinology. In most cases, the chapters include some discussion of shortcomings and advantages of a particular methodology, as well as future prospects. The goals are twofold: to encourage those not currently engaged in neuroendocrine research to realize the interesting potential of this field, and to offer new techniques for the consideration of those already in the field. This book is intended for students, residents, fellows, and others interested in neuroendocrine research. In most cases however, it is not a beginner's manual. With some exceptions, the chapters appear to assume an essential familiarity with the actual techniques. Among the clearest descriptions of methodology are the chapters on the use of reverse transcriptase-polymerase chain reaction to assess neuropeptide receptors, on the utility of biotinylated ligands to study binding in pituitary cells, and the description of in vivo electrophysiological approaches. An excellent analysis of the advantages and drawbacks of alternative methods for intracellular recording from hypothalamic cells is provided, and there is a thorough discussion of the issues related to quantifying pulsatility in neuroendocrine systems. Also discussed are the utility of primary neuronal cultures. Wetsel, Merchenthaler, and Liposits review their work with immortalized cells. For the reader interested in an overview of many of the current techniques used in neuroendocrine studies, this book provides someexcellent appraisals of their respective virtues and drawbacks. In contrast to some other methods books however, this compact volume seldom provides the extensive details that may be sought by someone wishing to put a new technique into practice.

Marie J. Gibson

"…Louis Van de Kar and a broad spectrum of researchers in neuroendocrinology have produced a useful handbook for those new to the field as well as neuroendocrinologists searching for novel approaches to old questions."
-Judith L. Turgeon, The Physiologist, October 1999

From the Publisher