Methods in Neurosciences: Peptidases and Neuropeptide Processing

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Molecular Approaches for the Study of Intracellular Processing Enzymes:
N.G. Seidah, Molecular Strategies for Identifying Processing Enzymes.
M.K.-H. Schefer and R. Day, In Situ Hybridization Techniques to Map Processing Enzymes.
M. Zheng and J.E. Pintar, Analysis of Ontogeny of Processing Enzyme Gene ...
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More About This Book

Overview

Molecular Approaches for the Study of Intracellular Processing Enzymes:
N.G. Seidah, Molecular Strategies for Identifying Processing Enzymes.
M.K.-H. Schefer and R. Day, In Situ Hybridization Techniques to Map Processing Enzymes.
M. Zheng and J.E. Pintar, Analysis of Ontogeny of Processing Enzyme Gene Expression and Regulation.
J.K. VanSlyke, L. Thomas, and G. Thomas, Use of Vaccinia Virus Vectors to Study Neuropeptide Processing.
I. Lindberg and Y. Zhou, Overexpression of Neuropeptide Precursors and Processing Enzymes.
R.E. Mains, Use of Antisense RNA to Block Peptide-Processing Enzyme Expression.
Immunological and Biochemical Approaches to the Study of Peptide-Processing Pathways:
A.I. Smith and R.A. Lew, Combination of High-Performance Liquid Chromatography and Radioimmunoassay for Characterization of Peptide-Processing Pathways.
S.R. Crosby, Development and Use of Two-Site Immunometric Assays for Examining Peptide-Processing Pathways.
P. Cohen, M. Rholam, and H. Boussetta, Methods for Identification of Neuropeptide-Processing Pathways.
H. Gainer, M.O. Lively, and M. Morris, Immunological and Related Techniques for Studying Neurohypophyseal Peptide-Processing Pathways.
R.G. Allen and J. Stack, Approaches to Assessing Ontogeny of Processing Enzymes.
R.A. Lew and A.I. Smith, Measurement, Distribution, and Subcellular Localization of Peptide-Amidating Activity.
L.D. Fricker, Methods for Studying Carboxypeptidase E.
T.J. Opgenorth, S. Kimura, and J.R. Wu-Wong, Characterization of Endothelin-Converting Enzymes.
A. Shulkes, In VivoApproaches for Studying Peptide Processing.
Identification and Characterization of Extracellular Processing Enzymes in the Central Nervous System:
J.R. McDermont and A.M. Gibson, Identification and Characterization of Central Nervous System Peptidase Activities.
M.J. Glucksman and J.L. Roberts, Strategies for Characterizing, Cloning, and Expressing Soluble Endopeptidases.
D.H. Small, G. Reed, S.J. Fuller, A. Weidemann, K. Beyreuther, and C.L. Masters, Proteolytic Processing and Amyloid Protein Precursor of Alzheimers Disease.
D.J. Campbell, A.C. Lawrence, A. Kladis, and A.-M.Duncan, Strategies for Measurement of Angiotensin and Bradykinin Peptides and Their Metabolites in Central Nervous System and Other Tissues.
A.J. Turner and K. Barnes, Distribution and Roles of Endopeptidase 24.11.
F. Checler, P. Dauch, H. Barelli, V. Dive, Y. Masuo, B. Vincent, and J.P. Vincent, Identification and Distribution of Endopeptidase 24.16 in the Central Nervous System.
S.Y. Chai and F.O. Mendelsohn, Autoradiographic Techniques to Map Angiotensin-Converting Enzyme in Brain and Other Tissues.
Index.

The book contains predominantly black-and-white illustrations, with some color illustrations.

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Editorial Reviews

From The Critics
Reviewer: Konstantin V. Slavin, MD(University of Illinois at Chicago College of Medicine)
Description: This book describes the current state-of-the-art techniques of laboratory processing of peptidases and neuropeptides. It systematically presents various approaches to both intracellular and extracellular processing enzymes and provides step-by-step protocols and procedures for enzyme studies.
Purpose: The goals are to summarize the latest techniques currently used in the authors' laboratories and to provide researchers with a starting point in developing strategies for the study of peptidases and neuropeptide processing. Because the area of applied neuroscience dealing with neuropeptides and peptidases is constantly expanding, this kind of practical summary of techniques is very valuable.
Audience: The book is written for both students and experimental researchers. It will be useful for neuroscientists who work with peptidases and neuropeptides as well as for those who plan or develop new projects in this field. The authors of the book are neuroscientists from seven countries actively involved in peptidase and neuropeptide processing research.
Features: The book has a sufficient amount of illustrations, mostly black-and-white. It is well referenced with separate reference lists after each chapter. The 22 chapters of the book are grouped into three sections, dealing with molecular aspects of intracellular processing enzymes, immunological and biochemical aspects of peptide-processing pathways, and techniques for extracellular processing enzymes in CNS. The book ends with a complete and helpful index.
Assessment: This is another volume in the recognized series Methods in Neurosciences, which is devoted to techniques of identification and characterization of peptidases and neuropeptide processing pathways. Written by an international cohort of leading experts in the field, it will be useful for researchers dealing with biochemical and molecular aspects of the nervous system. The book is recommended for biological and neuroscience libraries.
Konstantin V. Slavin
This book describes the current state-of-the-art techniques of laboratory processing of peptidases and neuropeptides. It systematically presents various approaches to both intracellular and extracellular processing enzymes and provides step-by-step protocols and procedures for enzyme studies. The goals are to summarize the latest techniques currently used in the authors' laboratories and to provide researchers with a starting point in developing strategies for the study of peptidases and neuropeptide processing. Because the area of applied neuroscience dealing with neuropeptides and peptidases is constantly expanding, this kind of practical summary of techniques is very valuable. The book is written for both students and experimental researchers. It will be useful for neuroscientists who work with peptidases and neuropeptides as well as for those who plan or develop new projects in this field. The authors of the book are neuroscientists from seven countries actively involved in peptidase and neuropeptide processing research. The book has a sufficient amount of illustrations, mostly black-and-white. It is well referenced with separate reference lists after each chapter. The 22 chapters of the book are grouped into three sections, dealing with molecular aspects of intracellular processing enzymes, immunological and biochemical aspects of peptide-processing pathways, and techniques for extracellular processing enzymes in CNS. The book ends with a complete and helpful index. This is another volume in the recognized series Methods in Neurosciences, which is devoted to techniques of identification and characterization of peptidases and neuropeptide processing pathways. Written by aninternational cohort of leading experts in the field, it will be useful for researchers dealing with biochemical and molecular aspects of the nervous system. The book is recommended for biological and neuroscience libraries.

3 Stars from Doody
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Product Details

  • ISBN-13: 9780121852931
  • Publisher: Elsevier Science & Technology Books
  • Publication date: 1/1/1995
  • Edition number: 1
  • Pages: 407

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