Methods in Nonradioactive Detection in Biological Systems / Edition 1

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The book summarizes principles and applications of the new radiotracers, focusing on both recently and approved new radiopharmaceuticals and those likely to be approved soon. A summary of clinical results is included.

This book contains predominantly black-and-white illustrations, with some color illustrations.

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Product Details

  • ISBN-13: 9780838569467
  • Publisher: McGraw-Hill Professional Publishing
  • Publication date: 1/1/1993
  • Edition number: 1
  • Pages: 366
  • Product dimensions: 7.62 (w) x 10.03 (h) x 0.82 (d)

Table of Contents

Sect. 1 The Basics of Nonradioactive Detection Systems 3
Sect. 2 General Considerations for Use of Manual 5
Sect. 3 Biotin Labeling Methods for Proteins and Carbohydrates 13
Sect. 4 Direct Conjugation of Enzymes to Proteins 29
Sect. 5 Labeling Proteins with Fluorochromes 39
Sect. 6 Comparing Fluorescent Organic Dyes for Biomolecular Labeling 47
Sect. 7 Chemiluminescent Detection Using Alkaline Phosphatase - The Chemistry 69
Sect. 8 Detection of Proteins on Filters by Enzymatic Methods 73
Sect. 9 Enzyme-Linked Immunosorbent Assays 89
Sect. 10 Automation of Protein and Immunochemistry Methods 111
Sect. 11 Biotin Labeling Methods for DNA 137
Sect. 12 Direct Attachment of Enzymes to DNA Probes 153
Sect. 13 Detection of Nucleic Acids on Filters by Enzymatic Methods 179
Sect. 14 Polymerase Chain Reaction and Its Impact on DNA Detection Limits 193
Sect. 15 Automated Procedures for DNA Methods 197
Sect. 16 Enzyme-Based Methods in Tissue Sections 211
Sect. 17 In Situ Hybridization 247
Sect. 18 Chromosome Mapping by Fluorescent in Situ Hybridization 263
Sect. 19 Colloidal Gold Localization of in Situ Hybrids 269
Sect. 20 Immunofluorescence Methods for Microscopic Analysis 293
Sect. 21 Flow Cytometry Methods 311
Index 337
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