Methods in Protein Structure Analysis / Edition 1

Methods in Protein Structure Analysis / Edition 1

by M. Zouhair Atassi

ISBN-10: 0306451247

ISBN-13: 9780306451249

Pub. Date: 01/31/1996

Publisher: Springer US

The MPSA international conference is held in a different country every two years. It is devoted to methods of determining protein structure with emphasis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of…  See more details below


The MPSA international conference is held in a different country every two years. It is devoted to methods of determining protein structure with emphasis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of the lOth MPSA decided to change the name to Methods in Protein Structure Analysis; however, the emphasis remains on "methods" and on "chemistry. " In fact, this is the only major conference that is devoted to methods. The MPSA conference is truly international, a fact clearly reflected by the composi­ tion of its Scientific Advisory Committee. The Scientific Advisory Committee oversees the scientific direction of the MPSA and elects the chairman of the conference. Members of the committee are elected by active members, based on scientific standing and activity. The chairman, subject to approval of the Scientific Advisory Committee, appoints the Organizing Committee. It is this latter committee that puts the conference together. The lectures of the MPSA have traditionally been published in a special proceedings issue. This is different from, and more detailed than, the special MPSA issue of the Journal of Protein Chemistry in which only a brief description of the talks is given in short papers and abstracts. In the I Oth MPSA, about half the talks are by invited speakers and the remainder were selected from submitted short papers and abstracts.

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Springer US
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Table of Contents

1Characterization of Proteins Separated by Gel Electrophoresis at the Primary Structure Level
2New Strategies in High Sensitivity Characterization of Proteins Separated From 1-D or 2-D Gels15
3High-Speed Chromatographic Separation of Proteins and Peptides for High Sensitivity Microsequence Analysis27
4Pre-Electrophoretic Labelling of Proteins with a Coloured Water Soluble Edman Reagent39
5High Sensitivity Analysis of PTH Amino Acids49
6Synthesis, Evaluation and Application of a Panel of Novel Reagents for Stepwise Degradation of Polypeptides57
7Identification and Characterization of Glycosylated Phenylthiohydantoin Amino Acids69
8Deacetylation and Internal Cleavage of Polypeptides for N-Terminal Sequences Analysis81
9A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vapor89
10Sequencing of Proteins from the C-Terminus109
11C-Terminal Protein Sequence Analysis Using the Hewlett-Packard G1009A C-Terminal Protein Sequencing System119
12Automated C-Terminal Sequencing of Polypeptides Containing C-Terminal Proline131
13Tandem Mass Spectrometric Characterization of Modified Peptides and Proteins141
14Direct Mass Spectrometric Analyses for Protein Chemistry Studies151
15Peptide-Mass Fingerprinting as a Tool for the Rapid Identification and Mapping of Cellular Proteins161
16The Use of Malditof Mass Spectrometry and Amino Acid Sequence Analysis in Characterising Small Amounts of N-Blocked Protein175
17Tracing Cell Signalling Pathways Using a Combination of 2D Gel Electrophoresis and Mass Spectrometry187
18Novel Flourescent Methods for Quantitative Determination of Monosaccharides and Sialic Acids in Glycoproteins by Reversed Phase High Performance Liquid Chromatography195
19Identification of the Disulfide Bonds of Human Complement Component C9: Comparison with the Other Components of the Membrane Attack Complex207
20Localization of in Vivo Phosphorylation Sites in Multiphosphorylated Proteins: Application of S-Ethylcysteine Derivation and Mass Spectrometric Analysis217
21Identification and Characterization of Transducin Functional Cysteines, Lysines, and Acidic Residues by Group-Specific Labeling and Chemical Cross-Linking227
22X-ray Photoelectron Spectroscopy of Amino Acids, Polypeptides and Simple Carbohydrates251
23Measurement of Asp/Asn Damage in Aging Proteins, Chemical Interconversion of Aspartyl Isomers, [superscript 18]O Tagging of Enzymatically Repaired Aspartyl Sites, and Enzyme Automethylation at Sites of Asp/Asn Damage261
24Purification Scheme for Isolation and Identification of Peptides Cross-Linked to the rRNA in Ribosomes275
25Investigation of Protein-Protein Interaction in Mitochondrial Steroid Hydroxylase Systems Using Site-Directed Mutagenesis283
26Modification of Chromatin Structure following Exposure of MOLT4 Cells to the Carcinogenic Chromium(VI)295
27Regions of Interaction between Nicotinic Acetylcholine Receptor and [alpha]-Neurotoxins and Development of a Synthtic Vaccine against Toxin Poisoning311
28Immunological Approach to Study the Structure of Oxidized Low Density Lipoproteins327
29Synthetic Combinatorial Libraries: A New Tool for Drug Design: Methods for Identifying the Composition of Compounds from Peptide and/or Nonpeptide Libraries
30DNA-Protein Interactions and Protein-Protein Interactions in Filamentous Bacteriophage Assembly: Implications for Epitope Display343
31Post-Translational Modification by Covalent Phosphorylation of Human Apolipoprotein B-100: Protein Kinase C-Mediated Regulation of Secreted apo B-100 in Hep G-2 Cells355
32Mixed Lineage Kinases: A New Family of Protein Kinases Containing a Double Leucine Zipper Domain, a Basic Motif and a SH3 Domain369
33Agonist-Induced Internalization and Degradation of [gamma]-Aminobutyric Acid[subscript A] (GABA[subscript A]) Receptor Polypeptides from the Neuronal Surface381
34Function and Structure of Human Leucocyte Collagenase389
35The Human DNA-Activated Protein Kinase, DNA-PK: Substrate Specificity395
36The P53 Tumor Suppressor Protein: Biophysical Characterization of the Carboxy-Terminal Oligomerization Domain407
37Distinctive Class Relationships within Vertebrate Alcohol Dehydrogenases419
38Primary Structure of a Novel Stylar RNase Unassociated with Self-Incompatibility in a Tobacco Plant, Nicotiana alata429
39Human Fibrinogen Occurs as over 1 Million Non-Identical Molecules435
40Microsequencing of Proteins of the Rough Endoplasmic Reticulum (RER) Membrane445
41Theoretical Studies of Protein Folding457
42Protein Sequence Analysis, Storage and Retrieval465
43Superfamily and Domain: Organization of Data for Molecular Evolution Studies473
44Mutational Analysis of the BPTI Folding Pathway483
45Three- and Four-Dimensional Heteronuclear NMR493
46Protein Docking in the Absence of Detailed Molecular Structures505
47A Metric Measure for Comparing Sequence Alignments515

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