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Methods of Protein Microcharacterization: A Practical Handbook / Edition 1

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Product Details

  • ISBN-13: 9780896030909
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 10/11/2007
  • Series: Biological Methods Series
  • Edition description: 1986
  • Edition number: 1
  • Pages: 456
  • Product dimensions: 9.00 (w) x 6.00 (h) x 1.19 (d)

Table of Contents

Microisolation Techniques.- Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography.- Reverse-Phase HPLC Isolation and Microsequence Analysis.- Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis.- Microamino Acid Analysis.- Amino Acid Analysis With Postcolumn Fluorescent Derivatization.- Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC.- Amino Terminal Analysis.- Manual Edman Sequencing System.- Comparison of the Spinning Cup and Gas-Phase Instruments.- Gas-Phase Protein/Peptide Sequencer.- Design of a Multipurpose Sequencer.- Solid-Phase Methods in Protein Microsequence Analysis.- Analysis of Phenylthiohydantoin Amino Acids by HPLC.- Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation.- Carboxyl-Terminal Analysis.- Microsequence Analysis by Enzymatic Methods.- COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods.- Mass Spectrometric Analysis of Polypeptides.- Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry.- Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.

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