Microbial Enzymes and Biotransformations / Edition 1

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Overview

Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic prools that show clearly how to go from laboratory results to successful industrial applications.

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Editorial Reviews

From the Publisher
The first two chapters alone are worth reading to appreciate the range of microbes and their applications in industry. Thereafter, each chapter deals with a particular topic and presents an introduction followed by an accurate set of methods, which are well set out and easy for the specialist to follow. However, this book would be a good addition to a library, although I suspect its comprehensive coverage of the subject will make it too general for personal purchase. -Robert D. Possee, CEH-Oxford

"Comprehensive and practical, this book offers laboratory and industrial scientists a wealth of proven enzymatic prools that show clearly how to go from laboratory results to successful industrial applications." - BioTech International

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Product Details

  • ISBN-13: 9781617374548
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Biotechnology Series, #17
  • Edition description: Softcover reprint of hardcover 1st ed. 2005
  • Edition number: 1
  • Pages: 320
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.74 (d)

Table of Contents

Microbial Cells and Enzymes: A Century of Progress
José L. Adrio and Arnold L. Demain

Enzyme Biosensors
Steven J. Setford and Jeffrey D. Newman

Directed Evolution of Enzymes: How to Improve Nucleic Acid Polymerases for Biocatalysis
Susanne Brakmann and Sascha Nico Stumpp

L-Glutaminase as a Therapeutic Enzyme of Microbial Origin
Abdulhameed Sabu, K. Madhavan Nampoothiri, and Ashok Pandey

Enzymatic Production of D-Amino Acids
Hemraj S. Nandanwar, Gurinder S. Hoondal, and Rakesh M. Vohra

Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides
Oskar Bañuelos, Maria del Carmen Ronchel, José Luis Adrio, and Javier Velasco

Food-Grade Corynebacteria for Enzyme Production
José A. Gil, Angelina Ramos, Sirin A. I. Adham, Noelia Valbuena, Michal Letek, and Luis M. Mateos

Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase
Yasuhisa Asano

Enzymes in Modern Detergents
Susumu Ito, Tohru Kobayashi, Yuji Hatada, and Koki Horikoshi

Microbial Proteases
Chandran Sandhya, K. Madhavan Nampoothiri, and Ashok Pandey

Proteases Produced by Halophilic Bacteria and Archaea
Encarnación Mellado, Cristina Sánchez-Porro, and Antonio Ventosa

Microbial Pectinases
Rupinder Tewari, Ram P. Tewari, and Gurinder S. Hoondal

Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes
Xin Gen Lei and Taewan Kim

Glucose Dehydrogenase for the Regeneration of NADPH and NADH
Andrea Weckbecker and Werner Hummel

Acetate Kinase from Methanosarcina thermophila, a Key Enzyme for Methanogenesis
Prabha Iyer and James G. Ferry

Immobilization of Enzymes by Covalent Attachment
Scott J. Novick and J. David Rozzell

Preparation of an IndustrialBiocatalyst of Penicillin G Acylase on Sepabeads: Improving the Design of Penicillin G Hydrolysis
Cesar Mateo, Valeria Grazu, Olga Abian, Manuel Fuentes, Gloria Fernández-Lorente, José M. Palomo, Roberto Fernández-Lafuente, and José M. Guisán

Methods for Bioencapsulation of Enzymes and Cells
Thomas M. S. Chang

Index

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