Microbiology: Laboratory Theory and Application, Brief / Edition 2


This brief version of the best-selling laboratory manual Microbiology: Laboratory Theory and Application, is intended for majors or non-majors in introductory microbiology laboratory courses. This full-color manual is appropriate for courses populated primarily by allied health students and courses with a preference for an abbreviated number of experiments.

Features of Microbiology: Laboratory Theory and Application, Brief, 2e:

  • NEW TO THIS EDITION - A new art program has been introduced that replaces all of the existing artwork from the previous edition
  • NEW TO THIS EDITION - Where possible, Biosafety level (BSL) 2 organisms have been replaced with equivalent BSL-1 organisms.
  • This version features more than 300 full-color, high quality, color-accurate photographs, micrographs, and illustrations.
  • Data sheets are incorporated into the experiments for ease of use.
  • Exercises are appropriate for both majors and non-majors courses and have been selected to provide exposure to lab experiences from all areas of microbiology.
  • The lab manual emphasizes the content and inclusion of skills as advised by the American Society for Microbiology in their Laboratory Core Curriculum.

Detailed list of changes to the new edition of Microbiology: Laboratory Theory and Application, Brief:

  • Section 1: A new, simple lab comparing the effectiveness of several hand-cleansing agents was added (Exercise 1-1). Exercise 1-4 (Common Aseptic Transfers) was rewritten. Procedural diagrams were also added as was extra emphasis on handling microbes in such a way as to reduce aerosols. Several new and replacement photos were added.
  • Section 2: Several new photos were added to improve this section. Exercise 2-13 (Chemical Germicides) was moved to the end of the section and was rewritten to include a more comprehensive procedure using BSL-1 organisms.
  • Section 3: Exercise 3-1 now includes two activities to supplement the theory and rules of microscope usage. Exercise 3-3 was totally reorganized to reflect current (but still provisional) classification of these organisms by "Eukaryotic Supergroups." Added to Exercise 3-6 (Gram Stain) is artwork illustrating the differences between Gram-positive and Gram-negative walls. The Acid-Fast Stain (Exercise 3-7) now includes the Ziehl-Neelsen protocol in addition to the Kinyoun method. Throughout, some older photomicrographs were replaced with new ones.
  • Section 4 - These exercises were reordered slightly to reflect a more reasonable approach to the material. The photo in Exercise 4-1 (Phenylethyl Alcohol Agar) was replaced with a better one and the Bile Esculin Test was moved to Section 5.
  • Section 5 - Several exercises, previously in Section 4 and Section 9, were moved to Section 5. Now included in this section are Bile Esculin Test (Exercise 5-10), PYR Test (Exercise 5-17), Bacitracin, Novobiocin, Optochin Tests (Exercise 5-20), and CAMP Test (Exercise 5-22).
  • Section 6 - New artwork and several new photos and/or photomicrographs improve the look of this section. The dilution schemes in this section have been rewritten for digital pipettes.
  • Section 7 - New antibiotics were added to the Kirby-Bauer Test (Exercise 7-2) as were a couple of new photos. The Morbidity and Mortality Weekly Report (Exercise 7-3) was updated and includes a photo of the CDC. The Epidemic Simulation (Exercise 7-4) was rewritten to place even greater emphasis on the techniques of safely executing the transfers without aerosol or droplet production.
  • Section 8 - Two exercises were removed: precipitin ring test and the dot blot. The latter was replaced with the Bio-Rad ELISA kit (Exercise 8-6).
  • Section 9 - Exercises 9-1, 9-2, and 9-3 (Identification of Unknown) were rewritten with new or revised identification flowcharts, necessitated by changing organism inventories at standard biological supply houses. An attempt was made to reduce the number of media required for each identification to provide more options for tests, and to reduce the number of BSL-2 organisms used.
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Product Details

  • ISBN-13: 9781617310287
  • Publisher: Morton Publishing Company
  • Publication date: 1/1/2012
  • Sold by: Barnes & Noble
  • Format: eTextbook
  • Edition number: 2
  • Pages: 576
  • File size: 31 MB
  • Note: This product may take a few minutes to download.

Table of Contents

1 Fundamental Skills for the Microbiology Laboratory
Exercise 1-1 A Comparison of Hand-Cleansing Agents
Exercise 1-2 Glo Germ Hand Wash Education System
Exercise 1-3 Nutrient Broth and Nutrient Agar Preparation
Exercise 1-4 Common Aseptic Transfers and Inoculation Methods
Exercise 1-5 Streak Plate Methods of Isolation
Exercise 1-6 Spread Plate Method of Isolation
2 Microbial Growth
Exercise 2-1 Ubiquity of Microorganisms
Exercise 2-2 Colony Morphology
Exercise 2-3 Growth Patterns on Slants
Exercise 2-4 Growth Patterns in Broth
Exercise 2-5 Evaluation of Media
Exercise 2-6 Fluid Thioglycollate Medium
Exercise 2-7 Anaerobic Jar
Exercise 2-8 The Effect of Temperature on Microbial Growth
Exercise 2-9 The Effect of pH on Microbial Growth
Exercise 2-10 The Effect of Osmotic Pressure on Microbial Growth
Exercise 2-11 Steam Sterilization
Exercise 2-12 The Lethal Effects of Ultraviolet Radiation on Microbial Growth
Exercise 2-13 Chemical Germicides: Disinfectants and Antiseptics
3 Microscopy and Staining
Exercise 3-1 Introduction to the Light Microscope
Exercise 3-2 Calibration of the Ocular Micrometer
Exercise 3-3 Microscopic Examination of Eukaryotic Microbes
Exercise 3-4 Simple Stains
Exercise 3-5 Negative Stains
Exercise 3-6 Gram Stain
Exercise 3-7 Acid-Fast Stain
Exercise 3-8 Capsule Stain
Exercise 3-9 Endospore Stain
Exercise 3-10 Bacterial Motility: Wet Mount and Hanging Drop Preparations
Exercise 3-11 Bacterial Motility: Flagella
Exercise 3-12 Morphological Unknown
4 Selective Media
Exercise 4-1 Phenylethyl Alcohol Agar
Exercise 4-2 Columbia CNA With 5% Sheep Blood Agar
Exercise 4-3 Mannitol Salt Agar
Exercise 4-4 MacConkey Agar
Exercise 4-5 Eosin Methylene Blue Agar
Exercise 4-6 Hektoen Enteric Agar
5 Differential Tests
Exercise 5-1 Oxidation-Fermentation Test
Exercise 5-2 Phenol Red Broth
Exercise 5-3 Methyl Red and Voges-Proskauer Tests
Exercise 5-4 Catalase Test
Exercise 5-5 Oxidase Test
Exercise 5-6 Nitrate Reduction Test
Exercise 5-7 Citrate Test
Exercise 5-8 Decarboxylation Test
Exercise 5-9 Phenylalanine Deaminase Test
Exercise 5-10 Bile Esculin Test
Exercise 5-11 Starch Hydrolysis
Exercise 5-12 Urea Hydrolysis
Exercise 5-13 Casein Hydrolysis Test
Exercise 5-14 Gelatin Hydrolysis Test
Exercise 5-15 DNA Hydrolysis Test
Exercise 5-16 Lipid Hydrolysis Test
Exercise 5-17 PYR Test
Exercise 5-18 SIM Medium
Exercise 5-19 Triple Sugar Iron Agar / Kligler Iron Agar
Exercise 5-20 Bacitracin, Novobiocin, and Optochin Susceptibility Tests
Exercise 5-21 Blood Agar
Exercise 5-22 CAMP Test
Exercise 5-23 Coagulase Tests
Exercise 5-24 Motility Test
6 Quantitative Techniques
Exercise 6-1 Standard Plate Count (Viable Count)
Exercise 6-2 Urine Culture
Exercise 6-3 Plaque Assay of Phage Titer
Exercise 6-4 Differential Blood Cell Count
Exercise 6-5 Environmental Sampling: The RODAC Plate
7 Medical Microbiology Introduction
Exercise 7-1 Snyder Test
Exercise 7-2 Antimicrobial Susceptibility Test (Kirby-Bauer Method)
Exercise 7-3 MMWR Assignment
Exercise 7-4 Epidemic Simulation
Exercise 7-5 Membrane Filter Technique
Exercise 7-6 Multiple Tube Fermentation Method for Total Coliform Determination
Exercise 7-7 Methylene Blue Reductase Test
Exercise 7-8 Making Yogurt
8 Microbial Genetics and Serology
Exercise 8-1 Extraction of DNA from Bacterial Cells
Exercise 8-2 Ultraviolet Radiation Damage and Repair
Exercise 8-3 Bacterial Transformation: The pGLO System
Exercise 8-4 Slide Agglutination
Exercise 8-5 Blood Typing
Exercise 8-6 ELISA For Detecting Antibodies in a Patient's Sample (The Antibody Capture Method)
9 Identification of Unknowns
Exercise 9-1 Identification of Enterobacteriaceae
Exercise 9-2 Identification of Gram-positive Cocci
Exercise 9-3 Identification of Gram-positive Rods
Exercise 9-4 API 20 E Identification System for Enterobacteriaceae and Other Gram-negative Rods
Exercise 9-5 Enterotube II
Exercise 9-6 Identification of Streptococcus and Streptococcus-like Organisms by Latex Agglutination: The Streptex Rapid Test

Appendix A: Biochemical Pathways
Appendix B: Miscellaneous Transfer Methods
Appendix C: Transfer from a Broth Culture Using a Glass Pipette
Appendix D: Transfers from a Broth Culture Using a Digital Pipette
Appendix E: Media, Reagent, and Stain Recipes
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