Microchip Capillary Electrophoresis: Methods and Protocols / Edition 1

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The text of this book is broken into four sections. The first section deals fabrication methods for the production of microchips as this is fundamental to the ability to use this technology. The next section discusses methods to control the surface chemistry and measure the resulting alterations in microfluidic devices. The third section of this book details different detection modes for microchip capillary electrophoresis with detail provided for mass spectrometry, electrochemistry, and finally conductivity. The final section of this book outlines applications of microchip capillary electrophoresis for biological analysis. Microchip Capillary Electrophoresis is intended to be both a practical guide for those interested in using this exciting new technology in their own research as well as providing important fundamental information detailing how the technique works at the molecular scale.

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Editorial Reviews

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"From this book the reader can acquire a basic command of the technique relatively fast and, if required, can move on to in-depth fundamental, or specific information in the literature of this explosively growing area of science." -Molecular Biotechnology
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Product Details

  • ISBN-13: 9781588292933
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/1/2006
  • Series: Methods in Molecular Biology Series, #339
  • Edition description: 2006
  • Edition number: 1
  • Pages: 238
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.63 (d)

Table of Contents

Part I. Microchip Fabrication Methods

Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes Mark M. Crain, Robert S. Keynton, Kevin M. Walsh, Thomas J. Roussel, Jr., Richard P. Baldwin, John F. Naber, and Douglas J. Jackson

Micro-Molding for Poly(dimethylsiloxane) Microchips Carlos D. García and Charles S. Henry

Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation Laurie E. Locascio, David J. Ross, Peter B. Howell, and Michael Gaitan

Part II. Surface Modification Methods

Surface Modification Methods for Enhanced Device Efficacy and Function Barbara J. Jones and Mark A. Hayes

Polyelectrolyte Coatings for Microchip Capillary Electrophoresis Yan Liu and Charles S. Henry

Part III. Detection Methods for Microchip Capillary Electrophoresis

Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry Trust Razunguzwa and Aaron T. Timperman

Interfacing Amperometric Detection With Microchip Capillary Electrophoresis R. Scott Martin

Conductivity Detection on Microchips Roland Hergenröder and Benedikt Graß

Part IV. Applications of Microchip Capillary Electrophoresis

DNA Separations Andrea W. Chow

Protein Separations Andrea W. Chow

Microchip Capillary Electrophoresis: Application to Peptide Analysis Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte

Measuring Electroosmotic Flow in Microchips and Capillaries S. Douglass Gilman and Peter J. Chapman

Single Cell Analysis on Microfluidic Devices Christopher T. Culbertson

Rapid DNA Amplification in Glass Microdevices Christopher J. Easley, Lindsay A. Legendre, James P. Landers, and Jerome P. Ferrance


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