Microchip Capillary Electrophoresis: Methods and Protocols / Edition 1

Microchip Capillary Electrophoresis: Methods and Protocols / Edition 1

by Charles Henry
     
 

Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Prools, leading chemists and engineers concisely explain the principles behind

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Overview

Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Prools, leading chemists and engineers concisely explain the principles behind this revolutionary technique and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. The authors describe in detail the detection methods used, including mass spectrometry, electrochemistry, and conductivity. Since surface chemistry must be carefully considered for optimal operation at this scale, they also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. The prools follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Instructive and well-detailed, Microchip Capillary Electrophoresis: Methods and Prools provides a practical starting point for both initiating research in the field of microchip capillary electrophoresis and understanding the full range of how these new systems can be used successfully in biological research.

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Product Details

ISBN-13:
9781617374760
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Biology Series, #339
Edition description:
Softcover reprint of hardcover 1st ed. 2006
Pages:
238
Product dimensions:
9.00(w) x 6.00(h) x 0.56(d)

Table of Contents

Part I. Microchip Fabrication Methods

Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes
Mark M. Crain, Robert S. Keynton, Kevin M. Walsh, Thomas J. Roussel, Jr., Richard P. Baldwin, John F. Naber, and Douglas J. Jackson

Micro-Molding for Poly(dimethylsiloxane) Microchips
Carlos D. García and Charles S. Henry

Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation
Laurie E. Locascio, David J. Ross, Peter B. Howell, and Michael Gaitan

Part II. Surface Modification Methods

Surface Modification Methods for Enhanced Device Efficacy and Function
Barbara J. Jones and Mark A. Hayes

Polyelectrolyte Coatings for Microchip Capillary Electrophoresis
Yan Liu and Charles S. Henry

Part III. Detection Methods for Microchip Capillary Electrophoresis

Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry
Trust Razunguzwa and Aaron T. Timperman

Interfacing Amperometric Detection With Microchip Capillary Electrophoresis
R. Scott Martin

Conductivity Detection on Microchips
Roland Hergenröder and Benedikt Graß

Part IV. Applications of Microchip Capillary Electrophoresis

DNA Separations
Andrea W. Chow

Protein Separations
Andrea W. Chow

Microchip Capillary Electrophoresis: Application to Peptide Analysis
Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte

Measuring Electroosmotic Flow in Microchips and Capillaries
S. Douglass Gilman and Peter J. Chapman

Single Cell Analysis on Microfluidic Devices
Christopher T. Culbertson

Rapid DNA Amplification in Glass Microdevices
Christopher J. Easley, Lindsay A. Legendre, James P. Landers, and Jerome P. Ferrance

Index

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