Microinjection and Transgenesis: Strategies and Protocols

Microinjection and Transgenesis: Strategies and Protocols

by Angel Cid-Arregui
     
 

The technique of microinjection is used to tranfer biological materials - RNA, proteins, oligonucleotides and especially cloned genes - into cultured somatic cells or embryos. Transgenic cells or animals - mice, rabbits or insects - allow the study of a wide range of cellular processes, such as gene expression, signal transduction or the cytoskeleton. More than 30

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Overview

The technique of microinjection is used to tranfer biological materials - RNA, proteins, oligonucleotides and especially cloned genes - into cultured somatic cells or embryos. Transgenic cells or animals - mice, rabbits or insects - allow the study of a wide range of cellular processes, such as gene expression, signal transduction or the cytoskeleton. More than 30 prools on microinjection experiments and transgenesis studies developed in renowned laboratories are described in this manual. Special topics are the generation of transgenic mice using YAC, ligand-dependent site-specific recombination in embryonic stem cells, morula aggregation to generate germline chimeras as well as the analysis of injected cells.

Product Details

ISBN-13:
9783540618959
Publisher:
Springer Berlin Heidelberg
Publication date:
12/28/1997
Series:
Springer Lab Manuals Series
Edition description:
Softcover reprint of the original 1st ed. 1998
Pages:
619

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