Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy / Edition 1

by M.A. Hayat

Overview

Hishemistry deals with the activities of chemical components in cells, and immunohishemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs.

This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.

Product Details

ISBN-13:	9780306467707
Publisher:	Springer US
Publication date:	06/30/2002
Edition description:	2002
Pages:	355
Product dimensions:	7.20(w) x 10.20(h) x 1.00(d)

Table of Contents

Preface. I. Introduction. II. Antigens and Antibodies. III. Fixation and Embedding. IV. Factors Affecting Antigen Retrieval. V. Problems in Antigen Retrieval. VI. Antigen Retrieval. VII. Antigen Retrieval on Resin Sections. VIII. General Methods for Antigen Retrieval. IX. Other Applications of Microwave Heating. X. Cell Proliferating Antigens. XI. Estrogens. XII. HER-2 (c-erbB-2) Oncoprotein. References. Subject Index.

Editorial Reviews

Hayat (Kean U.) provides laboratory protocols for the molecular investigation of diseases. The chemical and physical principles of using microwave heating for processing tissues for microscopy is explored. The retrieval and localization of antigens (more correctly, epitopes) in tissue is discussed for both normal and pathological situations. The highly visual histochemical approach is examined as a method for generating data not obtainable by the other methods. Annotation c. Book News, Inc., Portland, OR