Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy / Edition 1

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Overview

Hishemistry deals with the activities of chemical components in cells, and immunohishemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs.

This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.

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Editorial Reviews

From The Critics
Hayat (Kean U.) provides laboratory protocols for the molecular investigation of diseases. The chemical and physical principles of using microwave heating for processing tissues for microscopy is explored. The retrieval and localization of antigens (more correctly, epitopes) in tissue is discussed for both normal and pathological situations. The highly visual histochemical approach is examined as a method for generating data not obtainable by the other methods. Annotation c. Book News, Inc., Portland, OR
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Product Details

  • ISBN-13: 9780306467707
  • Publisher: Springer US
  • Publication date: 9/11/2008
  • Edition description: 2002
  • Edition number: 1
  • Pages: 355
  • Product dimensions: 7.20 (w) x 10.20 (h) x 1.00 (d)

Table of Contents

Preface. I. Introduction. II. Antigens and Antibodies. III. Fixation and Embedding. IV. Factors Affecting Antigen Retrieval. V. Problems in Antigen Retrieval. VI. Antigen Retrieval. VII. Antigen Retrieval on Resin Sections. VIII. General Methods for Antigen Retrieval. IX. Other Applications of Microwave Heating. X. Cell Proliferating Antigens. XI. Estrogens. XII. HER-2 (c-erbB-2) Oncoprotein. References. Subject Index.
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Sort by: Showing 1 Customer Reviews
  • Anonymous

    Posted July 16, 2002

    Review by Prof. dr. E Marani

    'This handbook is the most authorative publication in the disciplines of microscopy, immunohistochemistry and antigen retrieval methods. It is expected to become a classic in teaching and research. This book should not be absent in your personal collection, as well as the laboratory.' -Prof. dr. E Marani, Afdeling Fysiologie: Leiden, The Netherlands

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