BN.com Gift Guide

Microscopy, Optical Spectroscopy, and Macroscopic Techniques / Edition 1

Hardcover (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $1.99
Usually ships in 1-2 business days
(Save 98%)
Other sellers (Hardcover)
  • All (9) from $1.99   
  • New (5) from $106.06   
  • Used (4) from $1.99   

Overview

This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec­ troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand­ ing for the biochemist or biologist who needs to collaborate with spe­ cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol­ vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc­ ture using circular dichroism and Fourier-transform infrared spec­ troscopy.

Read More Show Less

Editorial Reviews

From the Publisher
...a quite fascinating book. It describes a range of techniques with which I am familiar in the literature, but have never actually used and explains the practicalities behind the instrumentation and its use...very readable and informative.-Dave Roberts, Natural History Museum-Society for General Microbiology Quarterly
Booknews
A survey of the possible applications of physicochemical techniques to the analysis of biological macromolecules, for practicing biochemists and biologists who want to use the technique but lack the experience. The contributors emphasize practical aspects, such as constraints on sample quantity, purity, and presentation; the problems of time and expense involved; the problems a technique is best suited to solve; and how the results may be interpreted. Among the methods examined are optical and infrared spectroscopy, microscopy, ultracentrifugation, calorimetry, X-ray and neutron scattering, and light scattering. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Read More Show Less

Product Details

  • ISBN-13: 9780896032323
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/27/1993
  • Series: Methods in Molecular Biology Series , #22
  • Edition description: 1994
  • Edition number: 1
  • Pages: 251
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.61 (d)

Table of Contents

Part I. Microscopy. Electron Microscopy of Protein-Nucleic Acid Complexes: Enhanced High-Resolution Shadowing. Visualization of Unshadowed DNA by Electron Microscopy: Adsorption to a Bacitracin Film. Biological Applications of Scanning Tunneling Microscopy. Part II.Scattering and Sedimentation: High Flux X-Ray and Neutron Solution Scattering. Determination of Macromolecular Homogeneity, Shape, and Interactions Using Sedimentation Velocity Analytical Ultracentrifugation. Determination of Absolute Molecular Weights Using Sedimentation Equilibrium Analytical Ultracentrifugation. Classical Light Scattering for the Determination of Absolute Molecular Weights and Gross Conformation of Biological Macromolecules. Determination of Diffusion Coefficients of Biological Macromolecules by Dynamic Light Scattering. Part III. Calorimetric Methods. Introduction to Microcalorimetry and Biomolecular Energetics. Differential Scanning Calorimetry. Isothermal Titration Microcalorimetry. Part IV. Optical Spectroscopy. Optical Spectroscopy: Principles and Instrumentation. The Measurement of Electronic Absorption Spectra in the Ultraviolet and Visible. Analysis of Polypeptide and Protein Structures Using Fourier Transform Infrared Spectroscopy. Fluorescence Spectroscopy. Circular Dichroism. Index.

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)