Gift Guide

Microtubules, in vitro

Hardcover (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $99.95
Usually ships in 1-2 business days
(Save 44%)
Other sellers (Hardcover)
  • All (3) from $99.95   
  • Used (3) from $99.95   
Sort by
Page 1 of 1
Showing All
Note: Marketplace items are not eligible for any coupons and promotions
Seller since 2005

Feedback rating:



New — never opened or used in original packaging.

Like New — packaging may have been opened. A "Like New" item is suitable to give as a gift.

Very Good — may have minor signs of wear on packaging but item works perfectly and has no damage.

Good — item is in good condition but packaging may have signs of shelf wear/aging or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Acceptable — item is in working order but may show signs of wear such as scratches or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Used — An item that has been opened and may show signs of wear. All specific defects should be noted in the Comments section associated with each item.

Refurbished — A used item that has been renewed or updated and verified to be in proper working condition. Not necessarily completed by the original manufacturer.

Very Good
2010 Hardcover Very Good 0123748151. This book is in very good condition; with remainder marks on edges. It does have some shelfwear. Inside pages are clean.; Methods in Cell ... Biology; 1.3 x 10.2 x 7.2 Inches; 450 pages. Read more Show Less

Ships from: Pflugerville, TX

Usually ships in 1-2 business days

  • Canadian
  • Standard, 48 States
  • Standard (AK, HI)
  • Express, 48 States
  • Express (AK, HI)
Seller since 2009

Feedback rating:


Condition: Very Good
Ex-Library hardcover in very nice condition with all the usual markings and attachments.

Ships from: Spring Branch, TX

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
  • Express, 48 States
  • Express (AK, HI)
Seller since 2005

Feedback rating:


Condition: Very Good
2010-06-15 Hardcover Very Good Ex-Library hardcover in very nice condition with all the usual markings and attachments.

Ships from: Spring Branch, TX

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
  • Express, 48 States
  • Express (AK, HI)
Page 1 of 1
Showing All
Sort by

More About This Textbook


There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.

* Chapters reflect bothexperimental procedures and new developments in the field of microtubule in vitro research.
* Combines classical approaches and modern technologies
* Presents easy-to-use protocols and thorough background information, compiled by leadersin the field

Read More Show Less

Product Details

  • ISBN-13: 9780123748157
  • Publisher: Elsevier Science
  • Publication date: 6/8/2010
  • Pages: 899
  • Product dimensions: 7.20 (w) x 10.20 (h) x 1.30 (d)

Table of Contents

Section1: Isolation, Biochemistry and Characterization of Antibodies and Isotypes.

  1. Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography
  2. Herbert P. Miller and Leslie Wilson

  3. Measurement Of Ligand Binding To Tubulin By Sulfhydryl Reactivity
  4. Adrian Begaye and Dan L Sackett

  5. Isolating Tubulin From Nonneural Sources
  6. Dan L. Sackett, Karl A. Werbovetz and Naomi S. Morrissette

  7. Characterization of Anti-b -Tubulin Antibodies
  8. Anthony J. Spano* and Anthony Frankfurter

  9. Expression profiling of tubulin isotypes and microtubule interacting proteins using real-time polymerase chain reaction
  10. Sharon Lobert, Laree Hiser and John J. Correia

  11. Nondenaturing Electrophoresis As A Tool To Investigate Tubulin Complexes
  12. Mónica López-Fanarraga, Gerardo Carranza, Raquel Castaño, Sofia Nolasco, J. Avila and J.C. Zabala

  13. Mass spectrometry analysis of c-terminal posttranslational Modifications of tubulins
  14. Redeker, Virginie

  15. Methods in Tubulin Proteomics
  16. Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard

    Section 2: Microtubule Structure and Dynamics

  17. Cryo-EM Studies of Microtubule Structural Intermediates and Kinetochore-Microtubule Interactions
  18. Eva Nogales, Vincent H. Ramey and Hong-Wei Wang

  19. High resolution imaging of microtubules and cytoskeleton structures by Atomic Force Microscopy
  20. Loic Hamon, Patrick A. Curmi, David Pastré

  21. Holly V. Goodson and Ivan V. Gregoretti
  22. Using computational modeling to understand microtubule dynamics: a primer for cell biologists

  23. Analysis of Dynamic Instability of Steady State Microtubules In Vitro by Video-Enhanced Differential Interference Contrast Microscopy with an Appendix by Emin Oroudjev
  24. Mythili Yenjerla, Manu Lopus and Leslie Wilson

  25. Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers
  26. Blake D. Charlebois, Henry T. Schek III, Alan J. Hunt

  27. Microtubule Dynamics Reconstituted in-vitro and Imaged y Single-Molecule Fluorescence Microscopy
  28. Christopher Gell, Volker Bormuth, Gary J. Brouhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schäffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, and Jonathon Howard

  29. Studying kinesin motors by optical 3D-nanometry in gliding motility assays
  30. Bert Nitzsche, Volker Bormuth, Corina Bräuer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez

    Section 3: Drugs

  31. Analysis of tubulin oligomers by Analytical Ultracentrifugation.
  32. J.J. Correia

  33. Determination of Drug Binding to Microtubules in vitro
  34. Jennifer A. Smith and Mary Ann Jordan

  35. Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions
  36. Bhabatarak Bhattacharyya, Sonia Kapoor and Dulal Panda

  37. A Tubulin polymerization micro-assay used to compare ligand efficacy
  38. Ashley Davis, Sarah Martinez, Daniel Nelson and Kim Middleton.

  39. Fluorescent Taxoid Probes for Microtubule Research
  40. Isabel Barasoain, J. Fernando Díaz, and José M. Andreu

  41. The binding of vinca domain agents to tubulin: structural and biochemical studies
  42. Anthony Cormie, Marcel Knossow, Chunguang Wang and Benoît Gigant

    Section 4: Interactions with Motors and MAPs

  43. Probing Interactions of Tubulin with Small Molecules, Peptides and Protein Fragments by Solution Nuclear Magnetic Resonance
  44. Marie-Jeanne Clément, Philippe Savarin, Elisabeth Adjadj, André Sobel, Flavio Toma, Patrick A. Curmi

  45. Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence and NMR
  46. François Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens and Vincent Peyrot

  47. Quantitative Analysis of MAP Mediated Regulation of Microtubule Dynamic Instability In Vitro - Focus on Tau
  48. Erkan Kiris, Donovan Ventimiglia and Stuart C. Feinstein

  49. Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy (FPM)
  50. Hernando Sosa, Ana B. Asenjo and Erwin J.G. Peterman

  51. Multiple color single molecule TIRF imaging and tracking of MAPs and motors
  52. Jennifer L. Ross and Ram Dixit

  53. Dixit and Ross - final submitted 11/4 & lost - resubmitted 1/8/10
  54. Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics
  55. Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler and Thomas Surrey

    Section 5: Functional Extracts and Force Measurements

  56. Quantitative Characterization of filament dynamics by single-molecule lifetime measurements.
  57. Leonid A. Mirny and Daniel J. Needleman

  58. Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle.
  59. Katja M. Taute, Francesco Pampaloni, Ernst-Ludwig Florin

  60. In vitro assays to study force generation at dynamic microtubule ends
  61. Liedewij Laan and Marileen Dogterom

  62. Reconstitution and functional analysis of kinetochore subcomplexes
  63. Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis and Linda Wordeman

  64. In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces

Ekaterina L. Grishchuk and Fazly I. Ataullakhanov

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)