Mitosis and Meiosis / Edition 1

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San Diego, CA 1998 Trade paperback New. This is spiral bound: BRAND NEW (NEVER OPENED)! ! -No international shipping available[GL5] Trade paperback (US). Glued binding. 489 p. ... Contains: Illustrations. Methods in Cell Biology, 61. Audience: General/trade. Read more Show Less

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Mitosis and Meiosis details the wide variety of methods currently used to study how cells divide in organisms as diverse as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle.
Chapters Contain Information On:
• How to generate, screen, and study mutants of mitosis in yeast, fingi, and flies
• Techniques to best image flourescent and nonfluorescent tagged dividing cells
• The use and action of mitoclastic drugs
• How to generate antibodies to mitotic components and inject them into cells
• Methods that can also be used to obtain information on cellular processes in nondividing cells

Audience: A broad audience of scientists, technicians, and graduate students in basic research, industry, and academic medicine, as well as those working in cell developmental biology, cancer research, genetics, and biochemistry.

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Editorial Reviews

Details many of the systems and methods currently used to study the two types of cell division in organisms from yeast to humans. Gives special attention to the spindle, a transient and highly dynamic structure that equally segregates the replicated chromosomes during both the mitosis of somatic cells and the meiosis of gametes. Among others methods, describes transmission electron, fluorescent speckle, and polarized light microscopy. Includes 12 pages of color plates. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780125885409
  • Publisher: Elsevier Science
  • Publication date: 12/16/1998
  • Series: Methods in Cell Biology Series
  • Edition description: SPIRAL
  • Edition number: 1
  • Pages: 489
  • Product dimensions: 7.86 (w) x 9.16 (h) x 0.94 (d)

Table of Contents

M. Moritz and B.M. Alberts, Isolation of Centrosomes from Drosophila Embryos.
M. Bornens and M. Moudjou, Studying the Composition and Function of Centrosomes in Vertebrates.
R.E. Palazzo and J.M. Vogel, Isolation of Centrosomes from Spisula solidissima Oocytes.
A. Van Hooser and B.R. Brinkley, Methods for the in Situ Localization of Proteins and DNA in the Centromere-Kinetochore Complex.
B.F. McEwen and M. Marko, Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research.
K.F. Sullivan, Enlightening Mitosis: Construction and Expression of GFP Fusion Proteins.
T. Wittmann and T. Hyman, Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes.
F. Severin, K. Kaplan, P. Sorger, and T. Hyman, In Vitro Assays for Studying S. cerevisiae Kinetochore Activity.
C. Waterman-Storer, A. Desai, and E.D. Salmon, Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells.
R. Oldenbourg, Polarized Microscopy of Spindles.
D. Zhang and R.B. Nicklas, Micromanipulation of Chromosomes and Spindles in Insect Spermatocytes.
P. Wadsworth, Microinjection of Mitotic Cells.
R. Kuriyama and K. Ensrud, Obtaining Antibodies to Spindle Components.
L. Wordeman and M. Wagenbach, Using Antisense Technology to Study Mitosis.
M.A. Jordan and L. Wilson, The Use and Action of Drugs in Analyzing Mitosis.
C.L. Rieder and G. Cassels, Correlative Light and Electron Microscopy of Mitotic Cells in Monolayer Cultures.
W.E. Theurkauf and M.M.S. Heck, Identification and Characterization of Mitotic Mutations in Drosophila.
B.R. Oakley, Methods of Isolating and Analyzing Mitotic Mutants in Aspergillus nidulans.
K. Bloom, D.L. Beach, P. Maddox, S.L. Shaw, E. Yeh, and E.D. Salmon, Using GFP Fusion Proteins to Quantitate Microtubule and Spindle Dynamics in Budding Yeast.
A. Desai, A. Murray, T. Mitchison, and C.E. Walczak, The Use of Xenopus Egg Extracts to Study Mitotic Spindle Assembly and Function in Vitro.
J.W. Voss, A.H. Valster, and P.K. Hepler, Methods for Studying Cell Division in Higher Plants.
G. Sluder, F.J. Miller, and E. Hinchcliffe, Using Sea Urchin Gametes for the Study of Mitosis.

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