Molecular Analysis of Cancer / Edition 1

Molecular Analysis of Cancer / Edition 1

by Jacqueline Boultwood
     
 

ISBN-10: 1617371025

ISBN-13: 9781617371028

Pub. Date: 12/12/2011

Publisher: Springer-Verlag New York, LLC

Molecular biotechnology has in recent years produced significant advances in finding tumor markers useful in diagnosis and in the assessment of disease progression and prognosis. In Molecular Analysis of Cancer, leading researchers in the field describe in step-by-step detail their best state-of-the-art molecular techniques for elucidating the biochemical processes…  See more details below

Overview

Molecular biotechnology has in recent years produced significant advances in finding tumor markers useful in diagnosis and in the assessment of disease progression and prognosis. In Molecular Analysis of Cancer, leading researchers in the field describe in step-by-step detail their best state-of-the-art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations. Techniques for the analysis of gene expression include microarray technology and differential display. All these techniques are of great value for identifying genes that are central to cancer development and progression.

Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities.

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Product Details

ISBN-13:
9781617371028
Publisher:
Springer-Verlag New York, LLC
Publication date:
12/12/2011
Series:
Methods in Molecular Medicine Series, #68
Edition description:
Softcover reprint of hardcover 1st ed. 2002
Pages:
304
Product dimensions:
6.00(w) x 9.00(h) x 0.66(d)

Table of Contents

Molecular Analysis of Cancer: An Overview
Ken Mills

Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization
Lyndal Kearney, Sabrina Tosi, and Rina J. Jaju

Spectral Karyotyping in Cancer Cytogenetics
Eva Hilgenfeld, Cristina Montagna, Hesed Padilla-Nash, Linda Stapleton, Kerstin Heselmeyer-Haddad, and Thomas Ried

Comparative Genomic Hybridization Analysis
Binaifer R. Balsara, Jianming Pei, and Joseph R. Testa

Detection of Chromosomal Deletions by Microsatellite Analysis
Rachel E. Ibbotson and Martin M. Corcoran

Detection and Quantification of Leukemia-Specific Rearrangements
Andreas Hochhaus

Detection of t(2;5)(p23;q35) Translocation by Long-Range PCR of Genomic DNA
Yunfang Jiang, L. Jeffrey Medeiros, and Andreas H. Sarris

Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer
Vorapan Sirivatanauksorn, Yongyut Sirivatanauksorn, Arthur B. McKie, and Nicholas R. Lemoine

Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism: Lessons from the ATM Gene
Igor Vorechovsky

Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis
Per Guldberg, Kirsten Grønbæk, Jesper Worm, Per thor Straten, and Jesper Zeuthen

Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay
Marianna Goldrick and James Prescott

Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay
Lucy Side

Mutation Analysis of Cancer Using Automated Sequencing
Amanda Strickson and Carrie Fidler

Detection of Differentially Expressed Genes in Cancer Using Differential Display
Yineng Fu

Genomewide Gene Expression Analysis Using cDNAMicroarrays
Chuang Fong Kong and David Bowtell

Gene Expression Profiling in Cancer Using cDNA Microarrays
Javed Khan, Lao H. Saal, Michael L. Bittner, Yuan Jiang, Gerald C. Gooden, Arthur A. Glatfelter, and Paul S. Meltzer

Wilms Tumor Gene WT1 as a Tumor Marker for Leukemic Blast Cells and Its Role in Leukemogenesis
Haruo Sugiyama
Detection of Aberrant Methylation of the p15INK4B Gene Promoter
Toshiki Uchida

Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon
John T. Phelan II and Josef T. Prchal

Telomere Length Changes in Human Cancer
Dominique Broccoli and Andrew K. Godwin

Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders
Kazuma Ohyashiki and Junko H. Ohyashiki

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