Table of Contents
Table of Contents
Part I. Laying the Foundation
1. An Overview of Biotechnology
2. Cell Properties and Processes
3. Genes, Genetics, and Geneticists
4. An Overview of Molecular Biology
5. Recombinant DNA Technology
Part II. Classroom Activities
A. DNA Structure and Function
6. Student Activity: Constructing a Paper DNA Model
7. Student Activity: DNA Replication
8. Student Activity: From Genes to Proteins
Student Activity: Making Antisense: Regulating Gene Expression with RNA
9. Student Activity: Sizes of the Escherichia coli and Human Genomes
10. Student Activity: Extraction of Bacterial DNA
B. Manipulation and Analysis of DNA
11. Student Activity: DNA Scissors
12. Student Activity: DNA Goes to the Races
13. Student Activity: Restriction Analysis of Lambda DNA
14. Student Activity: Recombinant Paper Plasmids
15. Student Activity: Restriction Analysis Challenge
16. Student Activity: Detection of Specific DNA Sequences: Part I. Fishing for DNA
Student Activity: Detection of Specific DNA Sequences: Part II. Combining Restriction and Hybridization Analysis
Student Activity: Detection of Specific DNA Sequences: Part III. Southern Hybridization
17. Student Activity: Paper PCR
18. Student Activity: DNA Sequencing: The Terminators
Reading: Chain Terminators as Antiviral Drugs
C. Transfer of Genetic Information
Reading: Gene Transfer, Escherichia coli, and Disease
19. Student Activity: Transformation of Escherichia coli
20. Student Activity: Conjugative Transfer of Antibiotic Resistance in Escherichia coli
Reading: Gene Transfer and the Spread of Antibiotic Resistance
21. Student Activity: Transduction of an Antibiotic Resistance Gene
22. Student Activity: Agrobacterium tumefaciens: Nature’s Plant Genetic Engineer
D. Molecular Biology and Genetics
23. Student Activity: An Adventure in Dog Hair, Part I
24. Student Activity: An Adventure in Dog Hair, Part II: Yellow Labs
25. Student Activity: Human Molecular Genetics
Reading: Molecular Genetics of Cancer
26. Student Activity: Genetics in Action
E. Genomics
27. Student Activity: Comparing Genomes
Exercise 1: STRs Can Cause an RFLP
Exercise 2: PCR Can Reveal Differences at Microsatellite Loci
Reading: Mitochondrial DNA
28. Student Activity: Forensic DNA Typing
Exercise 1. A Mix-Up at the Hospital
Exercise 2. A Paternity Case
Exercise 3. The Case of the Bloody Knife
Reading: DNA Forensics: An Archaeological Case
29. Student Activity: Mapping a Disease Gene
Reading: The Human Genome Project: Its Science, Applications, and Issues
30. Student Activity: Microarray Analysis of Genome Expression
Reading: Personal Genomics
F. Bioinformatics and Evolutionary Analysis of Proteins
31. Student Activity: Testing for Amylase Activity
32. Student Activity: Electrophoresis of Amylase Samples
33. Student Activity: Constructing an Amylase Evolutionary Tree
34. Student Activity: Exploring Bioinformatics
Part III. Societal Issues
Introduction to Societal Issues
35. Student Activity: Facing the Forces: Science, Technology, and Society
Reading: The Nature of Science
36. Student Activity: Analyzing the Issues of Biotechnology: Cloning
Reading: Are Bioengineered Foods Safe?
37. Student Activity: The Risks of Technology: Perception, Reality, or Both?
Reading: Biotechnology Regulation in the United States
38. Student Activity: Making Decisions When There is No Right Answer
39. Student Activity: Analyzing Ethical Dilemmas in Genetic Testing
Reading: Science, Law, and Politics
40. Student Activity: Analyzing Ethical Dilemmas in Gene Therapy
Part IV. Appendixes
A. Worksheets
B. Laboratory Biosafety
C. Aseptic Technique
D. Basic Microbiological Methods
