Molecular Biology & Cultural Heritage

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This book contains forty reviewed papers delivered at the International Congress on Molecular Biology and Cultural Heritage held in Seville, March 2003. It is divided in four parts, the first one presents the state-of-the-art and reviews molecular techniques applied to the study of microbial communities colonizing monuments and cultural heritage assets. Part two covers specific molecular techniques used in biodetereoration studies, part three includes an updated overview on on-going biodetereoration European Commission projects, and part four presents selected biodetereoration case studies from all over the world.

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Product Details

  • ISBN-13: 9789058095558
  • Publisher: Taylor & Francis
  • Publication date: 1/1/2003
  • Pages: 300
  • Product dimensions: 7.00 (w) x 10.00 (h) x 0.69 (d)

Meet the Author

Cesareo Saiz-Jimenez is a Research Professor at the Institute for Natural Resources and Agricultural Biology, Spanish National Research Council, Seville, Spain. He holds a Ph.D. in Biology (1975) from the University of Madrid, Spain, and in Chemical Engineering and Materials Sciences (1987) from the Technical University of Delft, The Netherlands. Since 1987 he has received funding from the European Commission, in Environment Research Programmes (1986-1994), Environment and Climate (1995-1998), The City of Tomorrow (1999-2002), and the 6FP and 7FP (2003-2013) as well as from national research programmes. He has participated in a total of 22 European projects and was national coordinator of EUROCARE (EUREKA programme), and Spanish manager of the City of Tomorrow and the Cultural Heritage, Key Action 4 from the European Commission. He is the current coordinator of the Spanish Network for Science and Technology for the Conservation of Cultural Heritage. This network comprises over 90 teams from universities, research centres, museums and SMEs. He has written over 450 papers published in international journals, congresses and workshops, as well as edited a number of books on different topics. Most of his research activity is aimed at the study of microbial communities in different terrestrial and aquatic ecosystems, biogeochemistry of terrestrial environments, and effects of air pollution on building materials. Particular attention was devoted to cultural heritage deterioration processes by microorganisms. In the last 20 years he has focused his research on the microbiology of subterranean environments (caves, ancient tombs, catacombs, mines). Papers issuing from this research describe e.g. the microbial ecology of caves such as Altamira in Spain, Lascaux in France and Grotta dei Cervi in Italy, microbe-mineral interactions, the biogeochemical cycle of elements, and the biodeterioration of rock art and mural paintings.

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Table of Contents

Overview on existing molecular techniques with potential interest in cultural heritage 3
Acidobacteria in Paleolithic painting caves 15
The colonisation of building materials by microorganisms as revealed by culturing and molecular methods 23
Description of novel bacterial species associated with biodeteriorated mural paintings using molecular techniques 29
Analysis of the microbial diversity present on the wall paintings of Castle of Herberstein by molecular techniques 35
Classifying bacterial isolates from hypogean environments: Application of a novel fluorimetric method for the estimation of G + C mol% content in microorganisms by thermal denaturation temperature 47
Fluorescent In Situ Hybridization (FISH) to study biodeterioration in cultural heritage 55
Biocide treatment of rock and mural paintings: Problems of application, molecular techniques of control and environmental hazards 61
Cyanobacteria and biodeterioration of monumental stones 73
Biodeterioration of miniature paintings from the 18th and 19th centuries 79
Microbial contamination in museum collections: Organic materials 85
Microbial toxins in moisture damaged indoor environments and cultural assets 93
Molecular characterization of lead-resistant isolates from Certosa of Pavia red stains 109
Characterization of bacterial communities on stone monuments by molecular biology tools 115
Protein studies in cultural heritage 119
Tiffany's drawings, fungal spots and phylogenetic trees 131
An investigation of bacterial dissolution of Maya limestone: Biodiversity and functional analysis 137
Molecular biology for investigation of cyanobacterial populations on historic buildings in Brazil 141
The potential of DGGE for analysis of fungal biofilms on historic buildings 145
Cyanobacteria attack rocks (CATS): Control and preventive strategies to avoid damage caused by cyanobacteria and associated microorganisms in Roman hypogean monuments
Aerophytic biofilms in dim habitats 163
The importance of light in the conservation of hypogean monuments 171
Biomineralisation of different crystalline phases by bacteria isolated from catacombs 179
Multiple approach to study the structure and diversity of microbial communities colonizing artistic surfaces. Study case: The Roman catacombs of St. Callixtus and Domitilla 187
Effect of Algophase on the cyanobacterium Gloeothece membranacea CCAP 1430/3 195
Biomediated calcite tratments for stone conservation 201
Microbial calcium carbonate precipitation for reinforcement of monumental stones 209
Fungal colonization on treated and untreated stone surfaces 213
Microbial communities in black crusts: An approach for assessing carbon utilisation 219
Insect infestation in museum collections: Organic materials 225
Biodeterioration control in cultural heritage: Methods and products 233
Deterioration and bioremediation of fresco: A case-study 243
Diagnostic tools for monitoring phototrophic biodeteriogens 247
A comparative study on biodeterioration and weathering effects in three sites of the Latin American cultural heritage 253
Microbiological studies of biofilm present on stones from the National Museum building, Bogota, Colombia 259
Experimental evidence of oxalate formation by fungal strains on marble samples 263
The microbial flora of naturally-aged silk fibroin 267
Atmospheric and biological deterioration of two churches of the cultural heritage of the city of Medellin, Colombia 271
Preliminary microbiological analysis of biofilms of some monuments in Villa de Leyva, Colombia 277
Experimental approach of the role of rock varnish in the transfer properties of sandstone 281
Author index 287
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