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-British Society for Developmental Biology Newsletter, Summer 2008, Vol. 29, No. 1
"Professors, lecturers, and instructors will find the fifth edition of the book Molecular Biology of the Cell and its accompanying Problems Book to be an excellent choice for guiding their students through the maze of the cell's molecular structures and biochemical processes....With countless colorful illustrations and a large number of photographs and tables, reading the text becomes not only an educational experience, but also a highly enjoyable one for those students who wish to discover the inner workings of the magnificent cellular machine....Educators will also find the DVD-ROM to be a rich electronic resource when compiling their lectures....No less important is the Problems Book, which contains numerous exercises and questions that are an integral part of the learning process, and that teachers, instructors, and students are sure to appreciate."
The Quarterly Review of Biology, September 2008, Volume 83, Number 3
All organisms, and all of the cells that constitute them, are believed to have descended from a common ancestor cell through evolution by natural selection. This involves two essential processes: (1) the occurrence of random variation in the genetic information passed from an individual to its descendants and (2) selection in favor of genetic information that helps its possessors to survive and propagate. Evolution is the central principle of biology, helping us to make sense of the bewildering variety in the living world.
This chapter, like the book as a whole, is concerned with the progression from molecules to multicellular organisms. It discusses the evolution of the cell, first as a living unit constructed from smaller parts and then as a building block for larger structures. Through evolution, we introduce the cell components and activities that are to be treated in detail, in broadly similar sequence, in the chapters that follow. Beginning with the origins of the first cell on earth, we consider how the properties of certaintypes of large molecules allow hereditary information to be transmitted and expressed and permit evolution to occur. Enclosed in a membrane, these molecules provide the essentials of a self-replicating cell. Following this, we describe the major transition that occurred in the course of evolution, from small bacteriumlike cells to much larger and more complex cells such as are found in present-day plants and animals. Lastly, we suggest ways in which single free-living cells might have given rise to large multicellular organisms, becoming specialized and cooperating in the formation of such intricate organs as the brain.
Clearly, there are dangers in introducing the cell through its evolution: the large gaps in our knowledge can be filled only by speculations that are liable to be wrong in many details. We cannot go back in time to witness the unique molecular events that took place billions of years ago. But those ancient events have left many traces for us to analyze. Ancestral plants, animals, and even bacteria are preserved as fossils. Even more important, every modern organism provides evidence of the character of living organisms in the past. Present-day biological molecules, in particular, are a rich source of information about the course of evolution, revealing fundamental similarities between the most disparate of living organisms and allowing us to map out the differences between them on an objective universal scale. These molecular similarities and differences present us with a problem like that which confronts the literary scholar who seeks to establish the original text of an ancient author by comparing a mass of variant manuscripts that have been corrupted through repeated copying and editing. The task is hard, and the evidence is incomplete, but it is possible at least to make intelligent guesses about the major stages in the evolution of living cells.
From Molecules to the First Cell 1
Simple Biological Molecules Can Form Under Prebiotic Conditions 1, 1 The conditions that existed on the earth in its first billion years are still a matter of dispute. Was the surface initially molten? Did the atmosphere contain ammonia, or methane? Everyone seems to agree, however, that the earth was a violent place with volcanic eruptions, lightning, and torrential rains. There was little if any free oxygen and no layer of ozone to absorb the ultraviolet radiation from the sun. The radiation, by its photochemical action, may have helped to keep the atmosphere rich in reactive molecules and far from chemical equilibrium. Simple organic molecules (that is, molecules containing carbon) are likely to have been produced under such conditions. The best evidence for this comes from laboratory experiments. If mixtures of gases such as COZ, CH4, NH3, and HZ are heated with water and energized by electrical discharge or by ultraviolet radiation, they react to form small organic molecules-usually a rather small selection, each made in large amounts (Figure 1-1). Among these products are compounds, such as hydrogen cyanide (HCN) and formaldehyde (HCHO), that readily undergo further reactions in aqueous solution (Figure 1-2). Most important, representatives of most of the major classes of small organic molecules found in cells are generated, including amino acids, sugars, and the purines and pyrimidines required to make nucleotides.
Although such experiments cannot reproduce the early conditions on the earth exactly, they make it plain that the formation of organic molecules is surprisingly easy. And the developing earth had immense advantages over any human experimenter; it was very large and could produce a wide spectrum of conditions. But above all, it had much more time-tens to hundreds of millions of years. In such circumstances it seems very likely that, at some time and place, many of the simple organic molecules found in present-day cells accumulated in high concentrations.
Complex Chemical Systems Can Develop in an Environment That Is Far from Chemical Equilibrium Simple organic molecules such as amino acids and nucleotides can associate to form polymers. One amino acid can join with another by forming a peptide bond, and two nucleotides can join together by a phosphodiester bond. The repetition of these reactions leads to linear polymers known as polypeptides and polynucleotides, respectively. In present-day living cells, large polyp eptides-known as proteins-and polynucleotides-in the form of both ribonucleic acids (RNA) and deoxyribonucleic acids (DNA)-are commonly viewed as the most important constituents. A restricted set of 20 amino acids constitute the universal building blocks of the proteins, while RNA and DNA molecules are constructed from just four types of nucleotides each. Although it is uncertain why these particular sets of monomers were selected for biosynthesis in preference to others that are chemically similar, we shall see that the chemical properties of the corresponding polymers suit them especially well for their specific roles in the cell.
The earliest polymers may have formed in any of several ways-for example, by the heating of dry organic compounds or by the catalytic activity of high concentrations of inorganic polyphosphates or other crude mineral catalysts. Under laboratory conditions the products of similar reactions are polymers of variable length and random sequence in which the particular amino acid or nucleotide added at any point depends mainly on chance (Figure 1-3). Once a polymer has formed, however, it can itself influence subsequent chemical reactions by acting as a catalyst.
The origin of life requires that in an assortment of such molecules there must have been some possessing, if only to a small extent, a crucial property: the ability to catalyze reactions that lead, directly or indirectly, to production of more molecules of the catalyst itself. Production of catalysts with this special self-promoting property would be favored, and the molecules most efficient in aiding their own production would divert raw materials from the production of other substances. In this way one can envisage the gradual development of an increasingly complex chemical system of organic monomers and polymers that function together to generate more molecules of the same types, fueled by a supply of simple raw materials in the environment. Such an autocatalytic system would have some of the properties we think of as characteristic of living matter: it would comprise a far from random selection of interacting molecules; it would tend to reproduce itself; it would compete with other systems dependent on the same feedstocks; and if deprived of its feedstocks or maintained at a wrong temperature that upsets the balance of reaction rates, it would decay toward chemical equilibrium and "die." But what molecules could have had such autocatalytic properties? In presentday living cells the most versatile catalysts are polypeptides, composed of many diff,prent amino acids with chemically diverse side chains and, consequently, able to adopt diverse three-dimensional forms that bristle with reactive sites. But although polypeptides are versatile as catalysts, there is no known way in which one such molecule can reproduce itself by directly specifying the formation of another of precisely the same sequence.
Polynucleotides Are Capable of Directing Their Own Synthesis 3
Polynucleotides have properties that contrast with those of polypeptides. They have more limited capabilities as catalysts, but they can directly guide the formation of exact copies of their own sequence...
|List of Topics|
|A Note to the Reader|
|Pt. I||Introduction to the Cell|
|1||Cells and Genomes||3|
|2||Cell Chemistry and Biosynthesis||47|
|Pt. II||Basic Genetic Mechanisms|
|4||DNA and Chromosomes||191|
|5||DNA Replication, Repair, and Recombination||235|
|6||How Cells Read the Genome: From DNA to Protein||299|
|7||Control of Gene Expression||375|
|8||Manipulating Proteins, DNA, and RNA||469|
|Pt. IV||Internal Organization of the Cell|
|11||Membrane Transport of Small Molecules and the Electrical Properties of Membranes||615|
|12||Intracellular Compartments and Protein Sorting||659|
|13||Intracellular Vesicular Traffic||711|
|14||Energy Conversion: Mitochondria and Chloroplasts||767|
|17||The Cell Cycle and Programmed Cell Death||983|
|18||The Mechanics of Cell Division||1027|
|Pt. V||Cells in their Social Context|
|19||Cell Junctions, Cell Adhesion, and the Extracellular Matrix||1065|
|20||Germ Cells and Fertilization||1127|
|21||Development of Multicellular Organisms||1157|
|22||Histology: The Lives and Deaths of Cells in Tissues||1259|
|24||The Adaptive Immune System||1363|
|25||Pathogens, Infection, and Innate Immunity||1423|
|Tables: The Genetic Code, Amino Acids|
Posted January 23, 2001
This book is perfect for the essentials of cell and molecular biology as taught at most major universities. It is also a very thorough reference for those working in the molecular biology field as a technician. The chapters are organized well with enough information to be able to assemble the 'big picture' and extract more from complicated and often convoluted scientific journals. The book is well written and very thorough and will satisfy even the most stringent requirements of any cell/molecular bio professor.Was this review helpful? Yes NoThank you for your feedback. Report this reviewThank you, this review has been flagged.