Molecular Cardiology: Methods and Protocols / Edition 1

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The first major collection of techniques for gene therapy and stem cell therapy for heart diseases. The methods include readily reproducible techniques for genetic dissection, signal transduction, and microarray analysis. The protocols include techniques for gene transfer to heart tissue using viral and nonviral vectors and for producing transgenic models of heart diseases, the novel and powerful tools for the study of heart disease pathogenesis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells. The techniques are at the center of the most recent advances in the study and treatment of genetic and acquired heart diseases.

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Product Details

  • ISBN-13: 9781617375217
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Medicine Series, #112
  • Edition description: Softcover reprint of hardcover 1st ed. 2005
  • Edition number: 1
  • Pages: 419
  • Product dimensions: 6.00 (w) x 9.00 (h) x 0.89 (d)

Table of Contents

Part I. Introduction

Introduction to Methods in Molecular Cardiology
Zhongjie Sun

Part II. Gene Transfer and Gene Therapy for Cardiovascular Disease

Gene Therapy Approaches to Cardiovascular Disease
J. Mark Jones and Walter J. Koch

Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System
Yi Tang

Cardiac Gene Delivery Using DNA-Adenoviral Constructs
J. Mark Jones and Walter J. Koch

Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology
Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, and Kaushik P. Patel

TAT-Mediated Protein Transduction: Delivering Biologically Active Proteins to the Heart
Åsa B. Gustafsson, Roberta A. Gottlieb, and David J. Granville

Expression of Human eNOS in Cardiac and Endothelial Cells
Xiuqing Wang, Robert Cade, and Zhongjie Sun

Strategies of Conditional Gene Expression in Myocardium: An Overview
Heather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor

Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes
Hideko Kasahara and Hiroki Aoki

Part III. Stem Cell Therapy for Cardiovascular Disease

Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair
Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Ménard, and Michel Pucéat

Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair
Yao Liang Tang

Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure
Yao Liang Tang

Prools for Myocardial Infarction Repair Using Fetal Cardiac Myocytes
Sharon Etzion, Radka Holbova, Liron Miller, and Jonathan Leor

Methods for Examining Stem Cells inPost-Ischemic and Transplanted Hearts
Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor

Endothelial Progenitor Cell Culture and Gene Transfer
Hideki Iwaguro and Takayuki Asahara

Part IV. Gene Analysis in the Injured and Hypertrophied Heart

Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy
Chellam Rajamanickam and Radhakrishnan Jeejabai.

Evaluation of the Cardiac Isoform of a2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy
Chellam Rajamanickam and Radhakrishnan Jeejabai

Detection of Apoptosis in Cardiovascular Diseases
Arwen L. Hunter, Jonathan C. Choy, and David J. Granville

Detection of Cardiac Signaling in the Injured and Hypertrophied Heart
Xiaoning Si, Maziar Rahmani, Ji Yuan, and Honglin Luo

Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart
Bobby Yanagawa, Lydia Taylor, Theresa A. Deisher, Raymond Ng, George F. Schreiner, Timothy J. Triche, Decheng Yang, and Bruce M. McManus

Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy
Bastien Llamas and Christian F. Deschepper

Induction and Analysis of Cardiac Hypertrophy in Transgenic Animal Models
Marcos E. Barbosa, Natalia Alenina, and Michael Bader

Methods for the Detection of Altered b-Adrenergic Receptor Signaling Pathways in Hypertrophied Hearts
Matthew J. Wolf, Hideo Tachibana, and Howard A. Rockman

Part V. Transgenes in Cardiovascular Research

Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation
Gopal J. Babu and Muthu Periasamy

Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes
Xia Shen, Gang Ye, Naira S. Metreveli, and Pau

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