Molecular Characterization Of Tgf Mediated Cancer Cell Proliferation

Molecular Characterization Of Tgf Mediated Cancer Cell Proliferation

by Garima Singh, Shiv Kishor Singh, Prof Dr Volker Ellenrieder
     
 

TGFβ has a dual role in carcinogenesis, acting as a growth inhibitor in early tumor stages and a promoter of cell proliferation in advanced diseases. Although this cellular phenomenon is well established, the underlying molecular mechanisms remain elusive. Here, we report that sequential induction of NFAT and c-Myc transcription factors is sufficient and required… See more details below

Overview

TGFβ has a dual role in carcinogenesis, acting as a growth inhibitor in early tumor stages and a promoter of cell proliferation in advanced diseases. Although this cellular phenomenon is well established, the underlying molecular mechanisms remain elusive. Here, we report that sequential induction of NFAT and c-Myc transcription factors is sufficient and required for the TGFβ switch from a cell cycle inhibitor to a growth promoter pathway in cancer cells. TGFβ induces the expression and activation of NFAT factors, which then translocate into the nucleus to promote c-Myc expression. In response to TGFβ, activated NFAT factors bind to and displace Smad3 repressor complexes from the c-Myc promoter. c-Myc in turn stimulates cell cycle progression and growth through up-regulation of D-type cyclins. Most importantly, NFAT knockdown not only prevents c-Myc activation and cell proliferation, but also partially restores TGF-beta-induced cell cycle arrest and growth suppression. Taken together, this study provides the first evidence for a Smad-independent master regulatory pathway in TGFβ-promoted cell growth that is defined by sequential transcriptional activation of NFAT and c-Myc factors.

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Product Details

ISBN-13:
9783639298017
Publisher:
AV Akademikerverlag GmbH & Co. KG.
Publication date:
11/04/2010
Pages:
100
Product dimensions:
6.00(w) x 9.00(h) x 0.24(d)

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