Molecular Cloning, A Laboratory Manual: Set of Volumes 1,2, and 3, Third Edition / Edition 3

Molecular Cloning, A Laboratory Manual: Set of Volumes 1,2, and 3, Third Edition / Edition 3

4.2 5
by Joseph Sambrook, David Russell, David W. Russell
     
 

ISBN-10: 0879695773

ISBN-13: 9780879695774

Pub. Date: 12/05/2000

Publisher: Cold Spring Harbor Laboratory Press

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its

Overview

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today’s biomolecular techniques.

The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.

These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.

The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.

The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved

Product Details

ISBN-13:
9780879695774
Publisher:
Cold Spring Harbor Laboratory Press
Publication date:
12/05/2000
Edition description:
3 Book Set
Pages:
2368
Product dimensions:
9.50(w) x 13.60(h) x 4.00(d)

Table of Contents

Chapter 1: Plasmids and Their Usefulness in Molecular Cloning

Chapter 2: Bacteriophage lambda and Its Vectors

Chapter 3: Working with Bacteriophage M13 Vectors

Chapter 4: Working with High-capacity Vectors

Chapter 5: Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis

Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA

Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells

Chapter 8: In vitro Amplification of DNA by the Polymerase Chain Reaction

Chapter 9: Preparation of Radiolabeled DNA and RNA Probes

Chapter 10: Working with Synthetic Oligonucleotide Probes

Chapter 11: Preparation of cDNA Libraries and Gene Identification

Chapter 12: DNA Sequencing

Chapter 13: Mutagenesis

Chapter 14: Screening Expression Libraries

Chapter 15: Expression of Cloned Genes in Escherichia coli

Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells

Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells

Chapter 18: Protein Interaction Technologies

Appendices

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >

Molecular Cloning, A Laboratory Manual: Set of Volumes 1,2, and 3, Third Edition 4.2 out of 5 based on 0 ratings. 5 reviews.
Ruud_van_Deursen More than 1 year ago
The book is wel recommended when you do Molecular cloning, but the product as advertised here is wrong. When I supposedly ordered the complete three volume set,  I was only delivered the first of the volumes. 
Guest More than 1 year ago
Any lab serious about molecular biology has this book. A lab staple for over 20 years the new updated version is even more comprehensive reflecting the continuing development of molecular biology. Most importantly the series still retains the historical significance and the background or reference material for many of the techniques. A must have!
Guest More than 1 year ago
A classic reference work in Molecular Biology, newly and completely updated. It has extensive coverage of nearly all core molecular biology techniques and often presents several different means of accomplishing the same goal. The website is easy to access and the protocols are clearly written. The website makes it easy to print out protocols. In addition each protocol contains references to the primary literature supporting its claims. In the internet version of the protocol the references are hotlinked to PubMed, greatly simplifying access to the underlying references. I definitely recommend this book to all labs using molecular biology. Make sure to check out the website!
Guest More than 1 year ago
Sambrook and Russell answer every question you could think of, and then some. This book is a goldmine of information, packed with protocols, but also filled with the extra information that transforms a simple set of instructions into an amazingly helpful how-to manual. The approach taken is that of an experimentor (ie. 'How to win the battle with RNase'--it really is a battle!), with hints and suggestions usually learned by watching an old pro. The information is well-organized, and very well illustrated to give a clear view of how an experiment is performed and especially the logic behind it. The manual also satisfies the insatiable curiosity of a scientist rather than a technician: how do pharmacological agents work? What are their structures? And the troubleshooting sections provide an invaluable resource. All in all, Sambrook and Russell have created an essential weapon in any scientist's artillery.
Guest More than 1 year ago
Molecular cloning has been a lab staple for years. Now reprinted so you can update the old lab copy worn out by years of student use! Its a must have for any lab serious about molecular biology. Its also useful for student training. Many times there are simple explanations for the lab techniques we have adopted as dogma, but are unsure why. Molecular cloning has the answers and is a great resource. I highly recommend this book for its depth and breadth of protocols and guidance in the complicated realm of cloning!