Molecular Cloning, A Laboratory Manual: Set of Volumes 1,2, and 3, Third Edition / Edition 3by Joseph Sambrook, David Russell, David W. Russell
Pub. Date: 12/05/2000
Publisher: Cold Spring Harbor Laboratory Press
In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.
In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.
Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today’s biomolecular techniques.
The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.
These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.
The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.
The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.
As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved
- Cold Spring Harbor Laboratory Press
- Publication date:
- Edition description:
- 3 Book Set
- Product dimensions:
- 9.50(w) x 13.60(h) x 4.00(d)
Table of Contents
- Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
- Chapter 2: Bacteriophage lambda and Its Vectors
- Chapter 3: Working with Bacteriophage M13 Vectors
- Chapter 4: Working with High-capacity Vectors
- Chapter 5: Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
- Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
- Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
- Chapter 8: In vitro Amplification of DNA by the Polymerase Chain Reaction
- Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
- Chapter 10: Working with Synthetic Oligonucleotide Probes
- Chapter 11: Preparation of cDNA Libraries and Gene Identification
- Chapter 12: DNA Sequencing
- Chapter 13: Mutagenesis
- Chapter 14: Screening Expression Libraries
- Chapter 15: Expression of Cloned Genes in Escherichia coli
- Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells
- Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
- Chapter 18: Protein Interaction Technologies
- Chapter 2: Bacteriophage lambda and Its Vectors
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Most Helpful Customer Reviews
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The book is wel recommended when you do Molecular cloning, but the product as advertised here is wrong. When I supposedly ordered the complete three volume set, I was only delivered the first of the volumes.
Any lab serious about molecular biology has this book. A lab staple for over 20 years the new updated version is even more comprehensive reflecting the continuing development of molecular biology. Most importantly the series still retains the historical significance and the background or reference material for many of the techniques. A must have!
A classic reference work in Molecular Biology, newly and completely updated. It has extensive coverage of nearly all core molecular biology techniques and often presents several different means of accomplishing the same goal. The website is easy to access and the protocols are clearly written. The website makes it easy to print out protocols. In addition each protocol contains references to the primary literature supporting its claims. In the internet version of the protocol the references are hotlinked to PubMed, greatly simplifying access to the underlying references. I definitely recommend this book to all labs using molecular biology. Make sure to check out the website!
Sambrook and Russell answer every question you could think of, and then some. This book is a goldmine of information, packed with protocols, but also filled with the extra information that transforms a simple set of instructions into an amazingly helpful how-to manual. The approach taken is that of an experimentor (ie. 'How to win the battle with RNase'--it really is a battle!), with hints and suggestions usually learned by watching an old pro. The information is well-organized, and very well illustrated to give a clear view of how an experiment is performed and especially the logic behind it. The manual also satisfies the insatiable curiosity of a scientist rather than a technician: how do pharmacological agents work? What are their structures? And the troubleshooting sections provide an invaluable resource. All in all, Sambrook and Russell have created an essential weapon in any scientist's artillery.
Molecular cloning has been a lab staple for years. Now reprinted so you can update the old lab copy worn out by years of student use! Its a must have for any lab serious about molecular biology. Its also useful for student training. Many times there are simple explanations for the lab techniques we have adopted as dogma, but are unsure why. Molecular cloning has the answers and is a great resource. I highly recommend this book for its depth and breadth of protocols and guidance in the complicated realm of cloning!