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Molecular Cloning: A Laboratory Manual, Fourth Edition / Edition 4
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Molecular Cloning: A Laboratory Manual, Fourth Edition / Edition 4

by Michael R. Green, Joseph R. Sambrook
 

ISBN-10: 1936113422

ISBN-13: 9781936113422

Pub. Date: 06/15/2012

Publisher: Cold Spring Harbor Laboratory Press

Molecular Cloning: A Laboratory Manual has always been the one indispensable molecular biology laboratory manual for protocols and techniques. The 4th edition of this classic manual preserves the detail and clarity of previous editions as well as the theoretical and historical underpinnings of the techniques presented. Ten original core chapters reflect

Overview

Molecular Cloning: A Laboratory Manual has always been the one indispensable molecular biology laboratory manual for protocols and techniques. The 4th edition of this classic manual preserves the detail and clarity of previous editions as well as the theoretical and historical underpinnings of the techniques presented. Ten original core chapters reflect developments and innovation in standard techniques and introduce new cutting-edge protocols. Twelve entirely new chapters are devoted to the most exciting current research strategies, including epigenetic analysis, RNA interference, genome sequencing, and bioinformatics. This manual is essential for both the inexperienced and the advanced user.

Product Details

ISBN-13:
9781936113422
Publisher:
Cold Spring Harbor Laboratory Press
Publication date:
06/15/2012
Edition description:
Lab Manual
Pages:
2028
Sales rank:
946,607
Product dimensions:
9.20(w) x 11.30(h) x 4.40(d)

Table of Contents

VOLUME 1
Part 1 Essentials
1. Isolation and Quantification of DNA
2. Analysis of DNA
3. Cloning and Transformation with Plasmid Vectors
4. Gateway Recombinational Cloning
John S. Reece-Hoyes and Albertha J.M. Walhout
5. Working with Bacterial Artificial Chromosomes and Other High-Capacity Vectors
Nathaniel Heintz and Shiaoching Gong
6. Extraction, Purification, and Analysis of RNA from Eukaryotic Cells
7. Polymerase Chain Reaction
8. Bioinformatics
Jui-Hung Hung and Zhiping Weng

VOLUME 2
Part 2 Analysis and Manipulation of DNA AND RNA
9. Quantification of DNA and RNA by Real-Time Polymerase Chain Reaction
10. Nucleic Acid Platform Technologies
Oliver Rando
11. DNA Sequencing
Elaine Mardis and W. Richard McCombie
12. Analysis of DNA Methylation in Mammalian Cells
Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee
13. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes
14. Methods for In Vitro Mutagenesis
Matteo Forloni, Alex Liu, and Narendra Wajapeyee

Part 3 Introducing Genes into Cells
15. Introducing Genes into Cultured Mammalian Cells
Priti Kumar, Arvindhan Nagarajan, and Pradeep D. Uchil
16. Introducing Genes into Mammalian Cells: Viral Vectors
Guangping Gao and Miguel Sena-Esteves

VOLUME 3
Part 4 Gene Expression
17. Analysis of Gene Regulation Using Reporter Systems
Pradeep D. Uchil, Arvindhan Nagarajan, and Priti Kumar
18. RNA Interference and Small RNA Analysis
Chengjian Li and Phillip D. Zamore
19. Expressing Cloned Genes for Protein Production, Purification, and Analysis
Clara L. Kielkopf, William Bauer, and Ina Urbatsch

Part 5 Interaction Analysis
20. Cross-Linking Technologies for Analysis of Chromatin Structure and Function
Tae Hoon Kim and Job Dekker
21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking Immunoprecipitation (CLIP)
Jennifer C. Darnell, Aldo Mele, Ka Ying Sharon Hung, and Robert B. Darnell
22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays
John S. Reece-Hoyes and Albertha J.M. Walhout

Appendices
1. Reagents and Buffers
2. Commonly Used Techniques
3. Detection Systems
4. General Safety and Hazardous Material

Index

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