Molecular Dermatology: Methods and Protocols

Overview

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases. In Molecular Dermatology: Methods and Prools, leading experts in the field provide a collection of state-of-the-art reliable prools covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas ...

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Overview

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases. In Molecular Dermatology: Methods and Prools, leading experts in the field provide a collection of state-of-the-art reliable prools covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Molecular Dermatology: Methods and Prools emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.

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Product Details

  • ISBN-13: 9781627032261
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/31/2012
  • Series: Methods in Molecular Biology Series , #961
  • Edition description: 2013
  • Edition number: 1
  • Pages: 460
  • Product dimensions: 7.10 (w) x 10.10 (h) x 1.30 (d)

Table of Contents

1. Molecular Dermatology Comes of Age

Cristina Has and Cassian Sitaru

2. Molecular Diagnosis of Autoimmune Blistering Diseases

Daisuke Tsuruta, Teruki Dainichi, Takahiro Hamada, Norito Ishii, and Takashi Hashimoto

3. Molecular Diagnosis of Genodermatoses

Vesarat Wessagowit

4. Three-Dimensional Visualization of the Molecular Architecture of Cell-Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections

Ashraf Al-Amoudi and Achilleas S. Frangakis

5. 3D Visualization of Epidermal Langerhans Cells

Akiharu Kubo, Keisuke Nagao, and Masayuki Amagai

6. In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography

Viviane Mumprecht and Michael Detmar

7. Optical Imaging of HPV Infection in a Murine Model

Rhonda C. Kines, Hisataka Kobayashi, Peter L. Choyke, and Marcelino L. Bernardo

8. Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration

Hagar I. Labouta, Ulrich F. Schaefer, and Marc Schneider

9. Analysis of Cutaneous Somatic Mosaicism

Anna M.G. Pasmooij and Marcel F. Jonkman

10. Global Proteome Analyses of SILAC Labeled Skin Cells

Adrian Sprenger, Victoria Küttner, Leena Bruckner-Tuderman, and Jörn Dengjel

11. MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real Time PCR Method

Janosch Hildebrand, Adam Grundhoff, Stefan Gallinat, Horst Wenck, and Anja Knott

12. Cell Death in the Skin: How to Study Its Quality and Quantity?

Roman Makarov, Peter Geserick, Maria Feoktistova, and Martin Leverkus

13. In Vitro Pathogenicity Assay for Anti-Desmoglein Autoantibodies in Pemphigus

Ken Ishii and Masayuki Amagai

14. Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo

Mircea Teodor Chiriac

15. Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model

Jean Albrengues, Guerrino Meneguzzi, and Cedric Gaggioli

16. Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues

Alexander D. Boiko

17. Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors

Benjamin Toh, Alessandra Nardin, Xilei Dai, Jo Keeble, Valerie Chew, and Jean-Pierre Abastado

18. Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Phoonvertible Fluorescent Protein “Kaede”-Transgenic Mice

Michio Tomura and Kenji Kabashima

19. Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach

Véronique J. Moulin

20. Bioengineered Skin Humanized Model of Psoriasis

Marta Carretero, Sara Guerrero-Aspizua, and Marcela Del Río

21. Induction of Contact Hypersensitivity in the Mouse Model

Stefan F. Martin

22. Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells

Ganna Bilousova and Dennis R. Roop

23. RNAi-Mediated Gene Function Analysis in Skin

Slobodan Beronja and Elaine Fuchs

24. Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies

Wataru Nishie

25. Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII

Alina Sesarman and Cassian Sitaru

26. Animal Model for Cutaneous Leishmaniasis

Michael R. Fischer, Dominik John, Kordula Kautz-Neu, Anja Schermann, Kirsten Schwonberg, and Esther von Stebut

27. Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils and Macrophages

Dominik John, Michael R. Fischer, and Esther von Stebut

28. Zebrafish as a Model System to Study Heritable Skin Diseases

Qiaoli Li and Jouni Uitto

29. Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa

Alexander Nyström, Leena Bruckner-Tuderman, and Johannes S. Kern

30. RNA Trans-Splicing for Genodermatoses

Johann W. Bauer, Eva M. Murauer, Verena Wally, and Ulrich Koller

Cristina Has and Cassian Sitaru

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