Molecular Diagnosis of Infectious Diseases / Edition 2

Molecular Diagnosis of Infectious Diseases / Edition 2

by Jochen Decker
     
 

ISBN-10: 161737430X

ISBN-13: 9781617374302

Pub. Date: 11/10/2010

Publisher: Springer-Verlag New York, LLC

In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and

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Overview

In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible prool is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references.

Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

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Product Details

ISBN-13:
9781617374302
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Medicine Series, #94
Edition description:
Softcover reprint of hardcover 2nd ed. 2004
Pages:
464
Product dimensions:
9.00(w) x 6.00(h) x 1.07(d)

Table of Contents

Part I. Protein-Based Identification of Antigens

Proteomic Approaches to Antigen Discovery
Karen M. Dobos, John S. Spencer, Ian M. Orme, and John T. Belisle

Immunoproteomics
Alexander Krah and Peter R. Jungblut

Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
Stephen Thompson

Part II. DNA-Based Identification of Antigens

Representational Difference Analysis of cDNA
Lucas D. Bowler

Microarray Data Analysis and Mining
Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, and Raffaele A. Calogero

Expression Cloning
Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, and Yasir A. W. Skeiky

Part III. Identification of Epitopes and Immunomodulatory Components

Determination of Epitopes by Mass Spectrometry
Christine Hager-Braun and Kenneth B. Tomer

Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays
Timothy W. Tobery and Michael J. Caulfield

Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity
Ludwig Deml, Jens Wild, and Ralf Wagner

Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, and Wulf Schneider-Brachert

Part IV. Expression of Recombinant Proteins

High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
Jutta Drees, Jason Smith, Frank Schäfer, and Kerstin Steinert

Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins
Markus Fuhrmann

Codon-Optimized Genes that Enable Increased Heterologous Expression inMammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
Marcus Graf, Ludwig Deml, and Ralf Wagner

Part V. Purification, Modification, and Renaturation of Recombinant Proteins

Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)
Udo Reischl

Purification of Recombinant Proteins with High Isoelectric Points
Raffaele A. Calogero and Anna Aulicino

Refolding of Inclusion Body Proteins
Marcus Mayer and Johannes Buchner

Small-Molecule-Protein Conjugation Procedures
Stephen Thompson

Part VI. Characterization of Recombinant Proteins

Structural Characterization of Proteins and Peptides
Rainer Deutzmann

Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
Claudia Hahnefeld, Stephan Drewianka, and Friedrich W. Herberg

Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
Uwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, and Hans Wolf

Part VII. Evaluation of Recombinant Proteins in Immunological Test Systems

Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
John E. Butler

Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
Alan Warnes, Anthony R. Fooks, and John R. Stephenson

Basic Problems of Serological Laboratory Diagnosis
Walter Fierz

Molecular Diagnostics Resources on the Internet
Larry Winger

Part VIII. Recombinant Receptor Molecules

Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
Lars Toleikis, Olaf Broders, and Stefan Dübel

Index

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